Module Use and Impact The Morphology and Imaging Module has two primary functions. First, it processes ocular and brain tissues for microscopy. This is a particularly valuable service for the physiologists and molecular biologists that do not have experience in histological techniques, but are very interested in imaging the tissues and cells that express genes or gene products they are investigating. Tissues are routinely processed for immunocytochemistry and in situ hybridization. Mr. Gillett devised a technique for plastic imbedding zebrafish embryos so the eyes would be stabilized and could be sectioned in the correct orientation. Second, this Module provides expertise for and access to equipment used for tissue processing, microscopy, and image analysis. An additional function of this Module is to provide training in the use of Module microscope and imaging equipment, tissue processing, in situ hybridization, immunohistological techniques, and image analysis software to participating investigators and their staff when requested. The Module will also assist with the planning and execution of immunohistochemistry experiments for investigators who lack the necessary expertise.

Agency
National Institute of Health (NIH)
Type
Center Core Grants (P30)
Project #
5P30EY007003-28
Application #
8689032
Study Section
Special Emphasis Panel (ZEY1)
Project Start
Project End
Budget Start
Budget End
Support Year
28
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of Michigan Ann Arbor
Department
Type
DUNS #
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109
Fernando, Roshini; Atkins, Stephen J; Smith, Terry J (2016) Intersection of Chemokine and TSH Receptor Pathways in Human Fibrocytes: Emergence of CXCL-12/CXCR4 Cross Talk Potentially Relevant to Thyroid-Associated Ophthalmopathy. Endocrinology 157:3779-3787
Puro, Donald G; Kohmoto, Ryohsuke; Fujita, Yasushi et al. (2016) Bioelectric impact of pathological angiogenesis on vascular function. Proc Natl Acad Sci U S A 113:9934-9
Sifuentes, Christopher J; Kim, Jung-Woong; Swaroop, Anand et al. (2016) Rapid, Dynamic Activation of Müller Glial Stem Cell Responses in Zebrafish. Invest Ophthalmol Vis Sci 57:5148-5160
Chen, Lisheng; Gage, Philip J (2016) Heterozygous Pitx2 Null Mice Accurately Recapitulate the Ocular Features of Axenfeld-Rieger Syndrome and Congenital Glaucoma. Invest Ophthalmol Vis Sci 57:5023-5030
Clark, Andrea J; Petty, Howard R (2016) WO3/Pt nanoparticles promote light-induced lipid peroxidation and lysosomal instability within tumor cells. Nanotechnology 27:075103
Korot, Edward; Comer, Grant; Steffens, Timothy et al. (2016) Algorithm for the Measure of Vitreous Hyperreflective Foci in Optical Coherence Tomographic Scans of Patients With Diabetic Macular Edema. JAMA Ophthalmol 134:15-20
Carver, Kyle A; Yang, Dongli (2016) N-Acetylcysteine Amide Protects Against Oxidative Stress-Induced Microparticle Release From Human Retinal Pigment Epithelial Cells. Invest Ophthalmol Vis Sci 57:360-71
Wu, Chris Y; Niziol, Leslie M; Musch, David C et al. (2016) Thyroid-Related Orbital Decompression Surgery: A Multivariate Analysis of Risk Factors and Outcomes. Ophthal Plast Reconstr Surg :
Clark, Andrea J; Petty, Howard R (2016) Identification of lesion subtypes in biopsies of ductal carcinoma in situ of the breast using biomarker ratio imaging microscopy. Sci Rep 6:27039
Zhao, Xiwu; Pack, Weston; Khan, Naheed W et al. (2016) Prolonged Inner Retinal Photoreception Depends on the Visual Retinoid Cycle. J Neurosci 36:4209-17

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