Abstract

In response to changing needs of VSRC investigators over the past year, including the addition of two senior faculty members with large programs in this area, Drs. Palczewski and Wensel, the Tissue Culture function was separated from Histology, and has now expanded to include a Hybridoma function. The Tissue Culture and Hybridoma (TCH) Module thus changed from the previous funding period in that Histology is now administratively with Microscopy and Imaging, and we have recruited a Co-Manager to run the Hybridoma facility since August 2006. This revised Module format is designed to provide VSRC investigators with facilities and expert technical assistance for: 1) culture and propagation of established and new cell lines of ocular tissues;and 2) development of monoclonal antibodies to proteins and other antigens of relevance to ocular disease.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Center Core Grants (P30)
Project #
5P30EY011373-13
Application #
7805444
Study Section
Special Emphasis Panel (ZEY1)
Project Start
Project End
Budget Start
2009-05-01
Budget End
2010-04-30
Support Year
13
Fiscal Year
2009
Total Cost
$165,444
Indirect Cost

  Institution

Name
Case Western Reserve University
Department
Type
DUNS #
077758407
City
Cleveland
State
OH
Country
United States
Zip Code
44106

  Publications

Gragg, Megan; Park, Paul S-H (2018) Misfolded rhodopsin mutants display variable aggregation properties. Biochim Biophys Acta Mol Basis Dis 1864:2938-2948
Parmar, Vipul M; Parmar, Tanu; Arai, Eisuke et al. (2018) A2E-associated cell death and inflammation in retinal pigmented epithelial cells from human induced pluripotent stem cells. Stem Cell Res 27:95-104
Cheng, Yu-Shiuan; Linetsky, Mikhail; Gu, Xilin et al. (2018) Light-induced generation and toxicity of docosahexaenoate-derived oxidation products in retinal pigmented epithelial cells. Exp Eye Res :
Palczewska, Grazyna; Stremplewski, Patrycjusz; Suh, Susie et al. (2018) Two-photon imaging of the mammalian retina with ultrafast pulsing laser. JCI Insight 3:
Mallory, D Paul; Gutierrez, Elizabeth; Pinkevitch, Margaret et al. (2018) The Retinitis Pigmentosa-Linked Mutations in Transmembrane Helix 5 of Rhodopsin Disrupt Cellular Trafficking Regardless of Oligomerization State. Biochemistry 57:5188-5201
Linetsky, Mikhail; Bondelid, Karina S; Losovskiy, Sofiya et al. (2018) 4-Hydroxy-7-oxo-5-heptenoic Acid Lactone Is a Potent Inducer of the Complement Pathway in Human Retinal Pigmented Epithelial Cells. Chem Res Toxicol 31:666-679
Choi, Elliot H; Suh, Susie; Sander, Christopher L et al. (2018) Insights into the pathogenesis of dominant retinitis pigmentosa associated with a D477G mutation in RPE65. Hum Mol Genet 27:2225-2243
Zhang, Lingjun; Li, Yan; Qiu, Wen et al. (2018) Targeting CD6 for the treatment of experimental autoimmune uveitis. J Autoimmun 90:84-93
Orban, Tivadar; Leinonen, Henri; Getter, Tamar et al. (2018) A Combination of G Protein-Coupled Receptor Modulators Protects Photoreceptors from Degeneration. J Pharmacol Exp Ther 364:207-220
Samanta, Amrita; Kiselar, Janna; Pumroy, Ruth A et al. (2018) Structural insights into the molecular mechanism of mouse TRPA1 activation and inhibition. J Gen Physiol 150:751-762

Showing the most recent 10 out of 510 publications