Abstract

The purpose of this module is to provide facilities for investigators to use two dimensional gel electrophoresis to separate complex mixtures of proteins that can then be analyzed by mass spectrometry. Spots on individual gels would be quantified by a fluorescence imaging system, 75 percent of the costs which would be paid for the Scripps Institute and 25 percent by the core grant. Software package for analysis of 2D gels would also be purchased, along with an in-gel digestion robot. Existing resources available to the core, Include MALDI-TOF, ESI-TOF, and LC/MS/MS Instruments.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Center Core Grants (P30)
Project #
1P30EY012598-01A1
Application #
6491616
Study Section
Special Emphasis Panel (ZEY1)
Project Start
2001-07-01
Project End
2006-06-30
Budget Start
Budget End
Support Year
1
Fiscal Year
2001
Total Cost
Indirect Cost

  Institution

Name
Scripps Research Institute
Department
Type
DUNS #
City
La Jolla
State
CA
Country
United States
Zip Code
92037

  Publications

McKeown, Caroline R; Nowak, Roberta B; Gokhin, David S et al. (2014) Tropomyosin is required for cardiac morphogenesis, myofibril assembly, and formation of adherens junctions in the developing mouse embryo. Dev Dyn 243:800-17
Gokhin, David S; Tierney, Matthew T; Sui, Zhenhua et al. (2014) Calpain-mediated proteolysis of tropomodulin isoforms leads to thin filament elongation in dystrophic skeletal muscle. Mol Biol Cell 25:852-65
Ochala, Julien; Gokhin, David S; Iwamoto, Hiroyuki et al. (2014) Pointed-end capping by tropomodulin modulates actomyosin crossbridge formation in skeletal muscle fibers. FASEB J 28:408-15
Gokhin, David S; Lewis, Raymond A; McKeown, Caroline R et al. (2010) Tropomodulin isoforms regulate thin filament pointed-end capping and skeletal muscle physiology. J Cell Biol 189:95-109
Johnson, Jennifer; Maher, Pamela; Hanneken, Anne (2009) The flavonoid, eriodictyol, induces long-term protection in ARPE-19 cells through its effects on Nrf2 activation and phase 2 gene expression. Invest Ophthalmol Vis Sci 50:2398-406
Nowak, Roberta B; Fischer, Robert S; Zoltoski, Rebecca K et al. (2009) Tropomodulin1 is required for membrane skeleton organization and hexagonal geometry of fiber cells in the mouse lens. J Cell Biol 186:915-28
Blacker, Adam; Mitchell, G Lynn; Bullimore, Mark A et al. (2009) Myopia progression during three years of soft contact lens wear. Optom Vis Sci 86:1150-3
Damek-Poprawa, Monika; Diemer, Tanja; Lopes, Vanda S et al. (2009) Melanoregulin (MREG) modulates lysosome function in pigment epithelial cells. J Biol Chem 284:10877-89
McKeown, Caroline R; Nowak, Roberta B; Moyer, Jeannette et al. (2008) Tropomodulin1 is required in the heart but not the yolk sac for mouse embryonic development. Circ Res 103:1241-8
Yang, Zhenglin; Chen, Yali; Lillo, Concepcion et al. (2008) Mutant prominin 1 found in patients with macular degeneration disrupts photoreceptor disk morphogenesis in mice. J Clin Invest 118:2908-16

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