The proposed Core facilities are designed to enhance and make more efficient the NEI-supported research carried out at the Massachusetts Eye and Ear Infirmary. The participating investigators conduct research involving the retina, retinal diseases, glaucoma, diseases and immunology of the cornea, and ocular development. The proposed Core is organized into the following four modules. 1. The Morphology Module provides resources, services, training, and technical assistance for investigators who require laser scanning confocal microscopy, transmission electron microscopy, light microscopy, immunofluorescence analysis, and related technologies. 2. The Molecular Biology Module provides services and equipment and DNA sequencing currently required for state-of-the-art molecular biology experiments involving vision science. 3. The Clinical Interface Module provides investigators with centralized and efficient processing of blood and other types of samples derived from patients who volunteer to participate in molecular genetics research and other research of the participating investigators. As such, it serves as the interface between clinicians who diagnose and treat patients with ocular disease and the clinician-scientists and basic scientists who wish to conduct experiments that require the analysis of DNA or other biological compounds derived from those patients. 4. The in Vivo Imaging and Function Module is a new module that will use non-invasive techniques to evaluate the status of ocular tissues in living animals used in vision research. The two technologies to be initially offered by this module will be optical coherence tomographic (OCT) imaging and electroretinographic (ERG) evaluation of retinal function. These methods will allow investigators to follow the course of ocular disease over time in individual animals, thereby reducing the number of animals needed for vision research as well as providing new insights into the physiology and pathology of ocular disease. This Core will augment our institution's commitment to devote major resources to the study of ocular diseases of its patients, especially diseases that still result in blindness despite current therapies, with the long-term goal of developing new therapies for these conditions.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Center Core Grants (P30)
Project #
5P30EY014104-10
Application #
8448717
Study Section
Special Emphasis Panel (ZEY1-VSN (08))
Program Officer
Liberman, Ellen S
Project Start
2002-04-01
Project End
2014-03-31
Budget Start
2013-04-01
Budget End
2014-03-31
Support Year
10
Fiscal Year
2013
Total Cost
$471,385
Indirect Cost
$171,140
Name
Massachusetts Eye and Ear Infirmary
Department
Type
DUNS #
073825945
City
Boston
State
MA
Country
United States
Zip Code
02114
Loomis, Stephanie J; Kang, Jae H; Weinreb, Robert N et al. (2014) Association of CAV1/CAV2 genomic variants with primary open-angle glaucoma overall and by gender and pattern of visual field loss. Ophthalmology 121:508-16
Yanai, Ryoji; Mulki, Lama; Hasegawa, Eiichi et al. (2014) Cytochrome P450-generated metabolites derived from ?-3 fatty acids attenuate neovascularization. Proc Natl Acad Sci U S A 111:9603-8
Wiggs, Janey L; Pawlyk, Basil; Connolly, Edward et al. (2014) Disruption of the blood-aqueous barrier and lens abnormalities in mice lacking lysyl oxidase-like 1 (LOXL1). Invest Ophthalmol Vis Sci 55:856-64
Hasegawa, Eiichi; Sweigard, Harry; Husain, Deeba et al. (2014) Characterization of a spontaneous retinal neovascular mouse model. PLoS One 9:e106507
Isayama, Tomoki; Chen, Ying; Kono, Masahiro et al. (2014) Coexpression of three opsins in cone photoreceptors of the salamander Ambystoma tigrinum. J Comp Neurol 522:2249-65
Matsumoto, H; Murakami, Y; Kataoka, K et al. (2014) Mammalian STE20-like kinase 2, not kinase 1, mediates photoreceptor cell death during retinal detachment. Cell Death Dis 5:e1269
Takeuchi, Kimio; Yanai, Ryoji; Kumase, Fumiaki et al. (2014) EGF-like-domain-7 is required for VEGF-induced Akt/ERK activation and vascular tube formation in an ex vivo angiogenesis assay. PLoS One 9:e91849
Kang, J H; Loomis, S J; Yaspan, B L et al. (2014) Vascular tone pathway polymorphisms in relation to primary open-angle glaucoma. Eye (Lond) 28:662-71
Bailey, Jessica N Cooke; Yaspan, Brian L; Pasquale, Louis R et al. (2014) Hypothesis-independent pathway analysis implicates GABA and acetyl-CoA metabolism in primary open-angle glaucoma and normal-pressure glaucoma. Hum Genet 133:1319-30
Matsumoto, Hidetaka; Kataoka, Keiko; Tsoka, Pavlina et al. (2014) Strain difference in photoreceptor cell death after retinal detachment in mice. Invest Ophthalmol Vis Sci 55:4165-74

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