The proposed Core facilities are designed to enhance and make more efficient the NEI-supported research carried out at the Massachusetts Eye and Ear Infirmary. The participating investigators conduct research involving the retina, retinal diseases, glaucoma, diseases and immunology of the cornea, and ocular development. The proposed Core is organized into the following four modules. 1. The Morphology Module provides resources, services, training, and technical assistance for investigators who require laser scanning confocal microscopy, transmission electron microscopy, light microscopy, immunofluorescence analysis, and related technologies. 2. The Molecular Biology Module provides services and equipment and DNA sequencing currently required for state-of-the-art molecular biology experiments involving vision science. 3. The Clinical Interface Module provides investigators with centralized and efficient processing of blood and other types of samples derived from patients who volunteer to participate in molecular genetics research and other research of the participating investigators. As such, it serves as the interface between clinicians who diagnose and treat patients with ocular disease and the clinician-scientists and basic scientists who wish to conduct experiments that require the analysis of DNA or other biological compounds derived from those patients. 4. The in Vivo Imaging and Function Module is a new module that will use non-invasive techniques to evaluate the status of ocular tissues in living animals used in vision research. The two technologies to be initially offered by this module will be optical coherence tomographic (OCT) imaging and electroretinographic (ERG) evaluation of retinal function. These methods will allow investigators to follow the course of ocular disease over time in individual animals, thereby reducing the number of animals needed for vision research as well as providing new insights into the physiology and pathology of ocular disease. This Core will augment our institution's commitment to devote major resources to the study of ocular diseases of its patients, especially diseases that still result in blindness despite current therapies, with the long-term goal of developing new therapies for these conditions.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Center Core Grants (P30)
Project #
5P30EY014104-10
Application #
8448717
Study Section
Special Emphasis Panel (ZEY1-VSN (08))
Program Officer
Liberman, Ellen S
Project Start
2002-04-01
Project End
2014-03-31
Budget Start
2013-04-01
Budget End
2014-03-31
Support Year
10
Fiscal Year
2013
Total Cost
$471,385
Indirect Cost
$171,140
Name
Massachusetts Eye and Ear Infirmary
Department
Type
DUNS #
073825945
City
Boston
State
MA
Country
United States
Zip Code
02114
Jamshidi, Farzad; Place, Emily M; Mehrotra, Sudeep et al. (2018) Contribution of noncoding pathogenic variants to RPGRIP1-mediated inherited retinal degeneration. Genet Med :
Lee, Jeong Yoon; Lee, Ji Sun; Materne, Emma C et al. (2018) Bacterial RecA Protein Promotes Adenoviral Recombination during In Vitro Infection. mSphere 3:
Kosmidou, Cassandra; Efstathiou, Nikolaos E; Hoang, Mien V et al. (2018) Issues with the Specificity of Immunological Reagents for NLRP3: Implications for Age-related Macular Degeneration. Sci Rep 8:461
Ismail, Ashrafali M; Lee, Ji Sun; Lee, Jeong Yoon et al. (2018) Adenoviromics: Mining the Human Adenovirus Species D Genome. Front Microbiol 9:2178
Bauer, Corinna M; Cattaneo, Zaira; Merabet, Lotfi B (2018) Early blindness is associated with increased volume of the uncinate fasciculus. Eur J Neurosci 47:427-432
Bailey, Jessica N Cooke; Gharahkhani, Puya; Kang, Jae H et al. (2018) Testosterone Pathway Genetic Polymorphisms in Relation to Primary Open-Angle Glaucoma: An Analysis in Two Large Datasets. Invest Ophthalmol Vis Sci 59:629-636
Fernandez-Godino, Rosario; Bujakowska, Kinga M; Pierce, Eric A (2018) Changes in extracellular matrix cause RPE cells to make basal deposits and activate the alternative complement pathway. Hum Mol Genet 27:147-159
Ismail, Ashrafali M; Cui, Tiange; Dommaraju, Kalpana et al. (2018) Genomic analysis of a large set of currently-and historically-important human adenovirus pathogens. Emerg Microbes Infect 7:10
Choi, Hee Joo; Wang, Rui; Jakobs, Tatjana C (2018) Single-Cell Dissociation and Characterization in the Murine Retina and Optic Nerve. Methods Mol Biol 1695:311-334
Paschalis, Eleftherios I; Lei, Fengyang; Zhou, Chengxin et al. (2018) Permanent neuroglial remodeling of the retina following infiltration of CSF1R inhibition-resistant peripheral monocytes. Proc Natl Acad Sci U S A 115:E11359-E11368

Showing the most recent 10 out of 296 publications