This is a proposal requesting Phase III support for five years to continue our successful COBRE Center for Protease Research (CPR) at North Dakota State University (NDSU), which was established in 2001 with Phase I of grant P20RR15566. The overarching goal for the Center is to obtain fundamental understanding of the role of proteases in disease in the broadest sense. During the first ten years, the Center has funded twenty-seven faculty researchers and established two core facilities. Several of these faculty members have received major research funding awards from NIH and NSF (R01, CAREER, and Presidential Young Investigator Awards) and the majority of the junior faculty have successfully competed for NIH (R03, R15, and R21), American Heart Association, or Department of Defense grants. In total, the Center PIs have received more than $17,500,000 in new funding and an additional $1,000,000 in education grants. The PIs from the Center have published 335 peer-reviewed publications since 2001. The strategic plan for Phase III of the CPR COBRE Center during the next five years is (1) to maintain and strengthen the state-of-the-art Core Biology and Core Synthesis Facilities established during Phases I and II that are essential for the support of the research projects in the Center, (2) to support research pilot projects and training components to sustain a collaborative, multidisciplinary research environment, (3) to provide significant mentoring in professional activities for the development of junior faculty members, and (4) to maintain an administrative core that will be required to coordinate and evaluate the entire program, including the Pilot Project Program and the two research cores. In this application, the Center for Protease Research is requesting funds to continue its support of two core facilities, to establish a new Pilot Project Program for biomedical research, and an Administrative Core to coordinate these activities. NDSU has clearly recognized the tremendous resources the Center brings to bear on its continued development as a research-intensive university. Over the course of the requested Phase III grant, NDSU will contribute $1,250,000 in direct support of the Center.

Public Health Relevance

(provided by applicant): All of the research projects supported by our Center are directed toward obtaining a fundamental understanding of the role of proteases in disease. Successful outcome of these activities should lead to improved treatments of human diseases such as cancer, asthma, and diabetes.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Center Core Grants (P30)
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Special Emphasis Panel (ZRR1-RI-B (01))
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Zlotnik, Hinda
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North Dakota State University
Schools of Arts and Sciences
United States
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Wu, Jingjing; Zhu, Yingyue; Xue, Feng et al. (2014) Recent trends in SELEX technique and its application to food safety monitoring. Mikrochim Acta 181:479-491
Singh, Neha; Vasam, Goutham; Pawar, Rahul et al. (2014) Angiotensin-(1-7) reverses angiogenic dysfunction in corpus cavernosum by acting on the microvasculature and bone marrow-derived cells in diabetes. J Sex Med 11:2153-63
Dhillon, Harsharan; Chikara, Shireen; Reindl, Katie M (2014) Piperlongumine induces pancreatic cancer cell death by enhancing reactive oxygen species and DNA damage. Toxicol Rep 1:309-318
Zhang, Q; Padi, S K R; Tindall, D J et al. (2014) Polycomb protein EZH2 suppresses apoptosis by silencing the proapoptotic miR-31. Cell Death Dis 5:e1486
Mahdi, Osama; Eklund, Bridget; Fisher, Nathan (2014) Laboratory culture and maintenance of Stenotrophomonas maltophilia. Curr Protoc Microbiol 32:Unit 6F.1.
Mei, Yang; Su, Minfei; Soni, Gaurav et al. (2014) Intrinsically disordered regions in autophagy proteins. Proteins 82:565-78
Su, Minfei; Mei, Yang; Sanishvili, Ruslan et al. (2014) Targeting ?-herpesvirus 68 Bcl-2-mediated down-regulation of autophagy. J Biol Chem 289:8029-40
Xu, Hui; Chen, Jiao; Birrenkott, Joseph et al. (2014) Gold-nanoparticle-decorated silica nanorods for sensitive visual detection of proteins. Anal Chem 86:7351-9
Mao, Xun; Gurung, Anant; Xu, Hui et al. (2014) Simultaneous detection of nucleic acid and protein using gold nanoparticles and lateral flow device. Anal Sci 30:637-42
Li, Bo; Liu, Qing; Xuan, Chengluan et al. (2013) GABAB receptor gene transfer into the nucleus tractus solitarii induces chronic blood pressure elevation in normotensive rats. Circ J 77:2558-66

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