The COBRE Phase I and II awards supported the creation of a state-of-the-art facility to provide the research infrastructure for all Center investigators encompassing molecular biology, quantitative imaging, immunohistochemistry, genomics and RIA of angiotensin peptides and provided most of the resources available in our state-of-the-art facilities. One of these facilities is the Molecular, Imaging, and Analytical Core Facility which provides a comprehensive service for molecular biology, imaging and biochemistry support. The core houses instruments and equipment needed to perform advanced molecular biology, semiquantitative immunohistochemistry and bio-analytical experiments. Among the instruments used by the Core are the real-time PCR machines, automatic BioRobot for RNA extraction, ELISA, microplate reader with fluorescence and bioluminescence capabilities, a flow cytometer, an automatic immunostainer, luminometer, gammacounter, multiprobe robotic liquid handling system for large scale RIA assays, an upright and two upright and one inverted fluorescent high resolution microscopes coupled to digital cameras and software for imaging capturing and analyses, and a fully functional tissue culture facility licensed for viral work. In addition, the core is also equipped with modern equipment for HPLC separation and analysis of angiotensin peptides which combined with RIA provides a means for quantitative analysis of angiotensin peptides and other hormonal factors in plasma, tissues and urine. The Molecular, Imaging, and Analytical Core facility has contributed greatly to the research projects conducted by the investigators of the Tulane Hypertension and Renal Center and has supported training of junior faculty, postdoctoral fellows, graduate and medical students in the areas of physiology, hypertension and cardiovascular and renal diseases. These outcomes have translated to a significant increase in the number of papers published in peer reviewed journals and have contributed to competitiveness for NIH and other agencies funded by our center members. During the past 9 years, over 350 papers supported by COBRE were published, including 110 papers since 2010.

Public Health Relevance

The COBRE Phase I and II awards supported the creation of a state-of-the-art facility to provide research infrastructure for all THRCE investigators. Modern research projects related to hypertension and related diseases often require the analysis of many factors that are altered by the hypertension process. This core facility provides the investigators the tools for evaluating the molecular, biochemical and structural mechanisms responsible for this pathological process.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Center Core Grants (P30)
Project #
Application #
Study Section
Special Emphasis Panel (ZRR1-RI-B)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Tulane University
New Orleans
United States
Zip Code
Rahman, Mahboob; Yang, Wei; Akkina, Sanjeev et al. (2014) Relation of serum lipids and lipoproteins with progression of CKD: The CRIC study. Clin J Am Soc Nephrol 9:1190-8
Scialla, Julia J; Xie, Huiliang; Rahman, Mahboob et al. (2014) Fibroblast growth factor-23 and cardiovascular events in CKD. J Am Soc Nephrol 25:349-60
Yoshida, Tadashi; Huq, Tashfin S; Delafontaine, Patrice (2014) Angiotensin type 2 receptor signaling in satellite cells potentiates skeletal muscle regeneration. J Biol Chem 289:26239-48
Miyata, Kayoko; Satou, Ryousuke; Inui, Daisuke et al. (2014) Renoprotective effects of direct renin inhibition in glomerulonephritis. Am J Med Sci 348:306-14
Miyata, Kayoko; Satou, Ryousuke; Shao, Weijian et al. (2014) ROCK/NF-?B axis-dependent augmentation of angiotensinogen by angiotensin II in primary-cultured preglomerular vascular smooth muscle cells. Am J Physiol Renal Physiol 306:F608-18
Gonzalez, Alexis A; Womack, Joel P; Liu, Liu et al. (2014) Angiotensin II increases the expression of (pro)renin receptor during low-salt conditions. Am J Med Sci 348:416-22
Hilliard, Sylvia A; Yao, Xiao; El-Dahr, Samir S (2014) Mdm2 is required for maintenance of the nephrogenic niche. Dev Biol 387:1-14
Blackstock, Christopher D; Higashi, Yusuke; Sukhanov, Sergiy et al. (2014) Insulin-like growth factor-1 increases synthesis of collagen type I via induction of the mRNA-binding protein LARP6 expression and binding to the 5' stem-loop of COL1a1 and COL1a2 mRNA. J Biol Chem 289:7264-74
Chirinos, Julio A; Khan, Abigail; Bansal, Nisha et al. (2014) Arterial stiffness, central pressures, and incident hospitalized heart failure in the chronic renal insufficiency cohort study. Circ Heart Fail 7:709-16
Navar, L Gabriel (2014) Physiology: hemodynamics, endothelial function, renin-angiotensin-aldosterone system, sympathetic nervous system. J Am Soc Hypertens 8:519-24

Showing the most recent 10 out of 29 publications