The COBRE Phase I and II awards supported the creation of a state-of-the-art facility to provide the research infrastructure for all Center investigators encompassing molecular biology, quantitative imaging, immunohistochemistry, genomics and RIA of angiotensin peptides and provided most of the resources available in our state-of-the-art facilities. One of these facilities is the Molecular, Imaging, and Analytical Core Facility which provides a comprehensive service for molecular biology, imaging and biochemistry support. The core houses instruments and equipment needed to perform advanced molecular biology, semiquantitative immunohistochemistry and bio-analytical experiments. Among the instruments used by the Core are the real-time PCR machines, automatic BioRobot for RNA extraction, ELISA, microplate reader with fluorescence and bioluminescence capabilities, a flow cytometer, an automatic immunostainer, luminometer, gammacounter, multiprobe robotic liquid handling system for large scale RIA assays, an upright and two upright and one inverted fluorescent high resolution microscopes coupled to digital cameras and software for imaging capturing and analyses, and a fully functional tissue culture facility licensed for viral work. In addition, the core is also equipped with modern equipment for HPLC separation and analysis of angiotensin peptides which combined with RIA provides a means for quantitative analysis of angiotensin peptides and other hormonal factors in plasma, tissues and urine. The Molecular, Imaging, and Analytical Core facility has contributed greatly to the research projects conducted by the investigators of the Tulane Hypertension and Renal Center and has supported training of junior faculty, postdoctoral fellows, graduate and medical students in the areas of physiology, hypertension and cardiovascular and renal diseases. These outcomes have translated to a significant increase in the number of papers published in peer reviewed journals and have contributed to competitiveness for NIH and other agencies funded by our center members. During the past 9 years, over 350 papers supported by COBRE were published, including 110 papers since 2010.

Public Health Relevance

The COBRE Phase I and II awards supported the creation of a state-of-the-art facility to provide research infrastructure for all THRCE investigators. Modern research projects related to hypertension and related diseases often require the analysis of many factors that are altered by the hypertension process. This core facility provides the investigators the tools for evaluating the molecular, biochemical and structural mechanisms responsible for this pathological process.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Center Core Grants (P30)
Project #
5P30GM103337-03
Application #
8708144
Study Section
Special Emphasis Panel (ZRR1-RI-B)
Project Start
Project End
Budget Start
2014-08-01
Budget End
2015-07-31
Support Year
3
Fiscal Year
2014
Total Cost
$217,709
Indirect Cost
$73,052
Name
Tulane University
Department
Type
DUNS #
053785812
City
New Orleans
State
LA
Country
United States
Zip Code
70118
Drawz, Paul E; Pajewski, Nicholas M; Bates, Jeffrey T et al. (2017) Effect of Intensive Versus Standard Clinic-Based Hypertension Management on Ambulatory Blood Pressure: Results From the SPRINT (Systolic Blood Pressure Intervention Trial) Ambulatory Blood Pressure Study. Hypertension 69:42-50
Scialla, Julia J; Asplin, John; Dobre, Mirela et al. (2017) Higher net acid excretion is associated with a lower risk of kidney disease progression in patients with diabetes. Kidney Int 91:204-215
Freedman, Barry I; Rocco, Michael V; Bates, Jeffrey T et al. (2017) APOL1 renal-risk variants do not associate with incident cardiovascular disease or mortality in the Systolic Blood Pressure Intervention Trial. Kidney Int Rep 2:713-720
Singh, Purnima; Castillo, Alexander; Islam, M Toriqul et al. (2017) Evidence for Prohypertensive, Proinflammatory Effect of Interleukin-10 During Chronic High Salt Intake in the Condition of Elevated Angiotensin II Level. Hypertension 70:839-845
Odden, Michelle C; Peralta, Carmen A; Berlowitz, Dan R et al. (2017) Effect of Intensive Blood Pressure Control on Gait Speed and Mobility Limitation in Adults 75 Years or Older: A Randomized Clinical Trial. JAMA Intern Med 177:500-507
Bazan, Hernan A; Hatfield, Samuel A; Brug, Aaron et al. (2017) Carotid Plaque Rupture Is Accompanied by an Increase in the Ratio of Serum circR-284 to miR-221 Levels. Circ Cardiovasc Genet 10:
Gao, Hong; Molinas, Adrien J R; Miyata, Kayoko et al. (2017) Overactivity of Liver-Related Neurons in the Paraventricular Nucleus of the Hypothalamus: Electrophysiological Findings in db/db Mice. J Neurosci 37:11140-11150
Zhan, Min; St Peter, Wendy L; Doerfler, Rebecca M et al. (2017) Patterns of NSAIDs Use and Their Association with Other Analgesic Use in CKD. Clin J Am Soc Nephrol 12:1778-1786
Berlowitz, Dan R; Foy, Capri G; Kazis, Lewis E et al. (2017) Effect of Intensive Blood-Pressure Treatment on Patient-Reported Outcomes. N Engl J Med 377:733-744
Rodriguez, Carlos J; Still, Carolyn H; Garcia, Katelyn R et al. (2017) Baseline blood pressure control in Hispanics: characteristics of Hispanics in the Systolic Blood Pressure Intervention Trial. J Clin Hypertens (Greenwich) 19:116-125

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