The goal of this core is to assist investigators with state-of-the-art technologies for 3-D tissue analysis and stereology. This core is the logical progression of the Molecular Neurobiology core, but now allows quantitation and analysis of cellular and molecular markers in tissue in 3-D. The LSUHSC-NCE has made a commitment to continue to capitalize, build, equip, and staff an Imaging Core Facility with state-of-the-art confocal and stereology microscopes and computers that can be used by the NCE and researchers from the surrounding biomedical community. It provides the technology and personnel to assist researchers in the preparation, embedding, sectioning, labeling, and contrast enhancement of tissue samples. Also with this technology, we can create 3-D images by reconstructing objects from optical or physical sections in 3-dimensions, archive this data, and quantitate them with state-of-the art microscopy and stereology software. This core will be of great help to newly hired NCE PI's as they initiate their research programs, and other researchers in different departments at LSUHSC, Tulane, and Xavier who need state-of-the-art imaging and stereology but don't have the resources or experience. This core will enable greater collaborations through imaging interactions, and additional R01, P50, and T32 grant applications. This facility will greatly sustain both the research community at LSUHSC and the greater New Orleans area. Starting in year 4, we will gradually transition to a fee-for-service facility so that by the end of the Sustainability Plan period this core would be fully self sustaining and independent. The three specific aims of this core are to: 1) hire and train a PhD level scientist to run this core and provide a graduate level seminar entitled: "Principles and Practice of Stereology and Confocal Microscopy";2) enable researchers to implement stereologic quantitation and whole-slide scanning using the new Olympus VS110 microscope and Visiopharm stereology software;and 3) operate the core to actualize COBRE Phase III project goals, and provide assistance to researchers and their projects throughout the process of design and implementation of 3-D confocal imaging.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Center Core Grants (P30)
Project #
5P30GM103340-03
Application #
8668110
Study Section
Special Emphasis Panel (ZRR1-RI-B)
Project Start
Project End
Budget Start
2014-06-01
Budget End
2015-05-31
Support Year
3
Fiscal Year
2014
Total Cost
$86,525
Indirect Cost
$26,438
Name
Louisiana State Univ Hsc New Orleans
Department
Type
DUNS #
782627814
City
New Orleans
State
LA
Country
United States
Zip Code
70112
Canavier, Carmen C (2015) Phase-resetting as a tool of information transmission. Curr Opin Neurobiol 31:206-13
Yu, Na; Tucker, Kristal R; Levitan, Edwin S et al. (2014) Implications of cellular models of dopamine neurons for schizophrenia. Prog Mol Biol Transl Sci 123:53-82
Hong, Sung-Ha; Belayev, Ludmila; Khoutorova, Larissa et al. (2014) Docosahexaenoic acid confers enduring neuroprotection in experimental stroke. J Neurol Sci 338:135-41
Zemski Berry, Karin A; Gordon, William C; Murphy, Robert C et al. (2014) Spatial organization of lipids in the human retina and optic nerve by MALDI imaging mass spectrometry. J Lipid Res 55:504-15
Li, Songhua; Izumi, Tadahide; Hu, Jane et al. (2014) Rescue of enzymatic function for disease-associated RPE65 proteins containing various missense mutations in non-active sites. J Biol Chem 289:18943-56
Li, Long; Tian, Xiaolin; Zhu, Mingwei et al. (2014) Drosophila Syd-1, liprin-?, and protein phosphatase 2A B' subunit Wrd function in a linear pathway to prevent ectopic accumulation of synaptic materials in distal axons. J Neurosci 34:8474-87
Bazan, Nicolas G (2014) Is there a molecular logic that sustains neuronal functional integrity and survival? Lipid signaling is necessary for neuroprotective neuronal transcriptional programs. Mol Neurobiol 50:1-5
Thounaojam, Umeshkanta S; Cui, Jianxia; Norman, Sharon E et al. (2014) Slow noise in the period of a biological oscillator underlies gradual trends and abrupt transitions in phasic relationships in hybrid neural networks. PLoS Comput Biol 10:e1003622
Tikidji-Hamburyan, Ruben; Lin, Eric C; Gasparini, Sonia et al. (2014) Effect of heterogeneity and noise on cross frequency phase-phase and phase-amplitude coupling. Network 25:38-62
Gordon, William C; Bazan, Nicolas G (2013) Mediator lipidomics in ophthalmology: targets for modulation in inflammation, neuroprotection and nerve regeneration. Curr Eye Res 38:995-1005

Showing the most recent 10 out of 18 publications