The goals for the Multiphoton Microscopy Core Facility are to make advancements on projects that were initiated during the first two phases of the COBRE program and to expand the use of the facility to investigators that require multiphoton microscopy for novel applications, but have limited experience with these techniques. COBRE investigators, in collaboration with other faculty at the Neuroscience Center of Excellence (NCE) have used our multiphoton imaging capabilities to make discoveries in hair cell synapses and plasticity of intrinsic and synaptic properties of dendrites in the hippocampus. The pioneering multiphoton imaging equipment and its applications attracted collaborations that included scientists from outside the Center and University, including Tulane University Medical School. These diverse projects illustrate the applications that can be considered with organized collaboration and core infrastructure. While extending the availability of multiphoton imaging to more researchers in the greater New Orleans area, ultimately the core will attract sufficient new funding, which, in addition to the institutional commitment of the School of Medicine, will make the core self-sufficient by the end of Phase III funding. The three specific aims of this core are: 1) To provide the infrastructure to sustain existing grant projects that listed the core as essential equipment in their NIH applications;2) To utilize the multiphoton facility to implement new research projects;enable collaborations between investigators;create new imaging applications in neuroscience;and help investigators obtain preliminary data for individual grant applications;3) To develop new research strategies by continuing to develop novel capabilities including optogenetics and in vivo imaging.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Center Core Grants (P30)
Project #
5P30GM103340-03
Application #
8668112
Study Section
Special Emphasis Panel (ZRR1-RI-B)
Project Start
Project End
Budget Start
2014-06-01
Budget End
2015-05-31
Support Year
3
Fiscal Year
2014
Total Cost
$69,834
Indirect Cost
$21,338
Name
Louisiana State Univ Hsc New Orleans
Department
Type
DUNS #
782627814
City
New Orleans
State
LA
Country
United States
Zip Code
70112
Canavier, Carmen C (2015) Phase-resetting as a tool of information transmission. Curr Opin Neurobiol 31:206-13
Yu, Na; Tucker, Kristal R; Levitan, Edwin S et al. (2014) Implications of cellular models of dopamine neurons for schizophrenia. Prog Mol Biol Transl Sci 123:53-82
Hong, Sung-Ha; Belayev, Ludmila; Khoutorova, Larissa et al. (2014) Docosahexaenoic acid confers enduring neuroprotection in experimental stroke. J Neurol Sci 338:135-41
Zemski Berry, Karin A; Gordon, William C; Murphy, Robert C et al. (2014) Spatial organization of lipids in the human retina and optic nerve by MALDI imaging mass spectrometry. J Lipid Res 55:504-15
Li, Songhua; Izumi, Tadahide; Hu, Jane et al. (2014) Rescue of enzymatic function for disease-associated RPE65 proteins containing various missense mutations in non-active sites. J Biol Chem 289:18943-56
Li, Long; Tian, Xiaolin; Zhu, Mingwei et al. (2014) Drosophila Syd-1, liprin-?, and protein phosphatase 2A B' subunit Wrd function in a linear pathway to prevent ectopic accumulation of synaptic materials in distal axons. J Neurosci 34:8474-87
Bazan, Nicolas G (2014) Is there a molecular logic that sustains neuronal functional integrity and survival? Lipid signaling is necessary for neuroprotective neuronal transcriptional programs. Mol Neurobiol 50:1-5
Thounaojam, Umeshkanta S; Cui, Jianxia; Norman, Sharon E et al. (2014) Slow noise in the period of a biological oscillator underlies gradual trends and abrupt transitions in phasic relationships in hybrid neural networks. PLoS Comput Biol 10:e1003622
Tikidji-Hamburyan, Ruben; Lin, Eric C; Gasparini, Sonia et al. (2014) Effect of heterogeneity and noise on cross frequency phase-phase and phase-amplitude coupling. Network 25:38-62
Gordon, William C; Bazan, Nicolas G (2013) Mediator lipidomics in ophthalmology: targets for modulation in inflammation, neuroprotection and nerve regeneration. Curr Eye Res 38:995-1005

Showing the most recent 10 out of 18 publications