The COBRE Proteogenomics and Bioinformatics Core C is a component of the South Carolina COBRE for Cardiovascular Disease (SC COBRE). The mission of this Core is to provide investigators from Medical University of South Carolina (MUSC) and across the region with expert consultation, training, state-of-the-art instrumentation and turnkey services to address questions of gene expression and protein function. The Core builds upon the previous investments of the NIH/NCRR SC COBRE Phase I and II programs, NIH/NCRR SC INBRE program, NIH/NCRR Shared Instrumentation Grants and MUSC, which together have created a comprehensive infrastructure comprised of faculty support staff, instrumentation and computational/statistical analysis resources. To advance our understanding of how gene expression relates to embryonic development and adult disease, the Core has excelled in microarray screening technology. To support this activity, the Core has built a record of accomplishment in data management and analysis and custom development of bioinformatics tools. The Core also provides support for related gene expression methodologies including RNA preparation and quality assessment, real time quantitative PCR analysis, chromatin immunoprecipitation (ChIP) and ChlP-on-Chip for analyzing genomic DNA sequences bound to specific proteins and epigenetic modifications. To advance our genomics capabilities, the Core will implement next generation sequencing in FY2011-12. The Core also provides a variety of protein related services including surface plasmon resonance (SPR) analysis of protein interactions, multiplex bead array-based analysis of phosphoprotein and cytokine levels, and consultation for expression/purification of proteins. Education and outreach are integral components of the mission of the Core. Toward this end, the Core provides a variety of programs for students and faculty that include training in Core technologies, research internships for undergraduates, pilot studies awards to incentivize Core usage, and a seminar series promoting new technology awareness. Collectively, the services of the Proteogenomics and Bioinformatics Core are designed to accelerate the process of discovery and enhance research competitiveness of investigators from MUSC and across the state.

Public Health Relevance

The COBRE MUSC Proteogenomics and Bioinformatics Core is a regional core facility that provides advanced genomic, proteomic and bioinformatic services and educational outreach activities that broadly benefit biomedical research across the state of South Carolina.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Center Core Grants (P30)
Project #
5P30GM103342-03
Application #
8657067
Study Section
Special Emphasis Panel (ZRR1-RI-B)
Project Start
Project End
Budget Start
2014-05-01
Budget End
2015-04-30
Support Year
3
Fiscal Year
2014
Total Cost
$292,149
Indirect Cost
$92,150
Name
Medical University of South Carolina
Department
Type
DUNS #
183710748
City
Charleston
State
SC
Country
United States
Zip Code
29425
Daoud, Abdelkader; Gopal, Udhayakumar; Kaur, Jasmine et al. (2017) Molecular and functional crosstalk between extracellular Hsp90 and ephrin A1 signaling. Oncotarget 8:106807-106819
Beiko, Tatsiana; Janech, Michael G; Alekseyenko, Alexander V et al. (2017) Serum Proteins Associated with Emphysema Progression in Severe Alpha-1 Antitrypsin Deficiency. Chronic Obstr Pulm Dis 4:204-216
Ghatak, Shibnath; Markwald, Roger R; Hascall, Vincent C et al. (2017) Transforming growth factor ?1 (TGF?1) regulates CD44V6 expression and activity through extracellular signal-regulated kinase (ERK)-induced EGR1 in pulmonary fibrogenic fibroblasts. J Biol Chem 292:10465-10489
Pulkoski-Gross, Michael J; Uys, Joachim D; Orr-Gandy, K Alexa et al. (2017) Novel sphingosine kinase-1 inhibitor, LCL351, reduces immune responses in murine DSS-induced colitis. Prostaglandins Other Lipid Mediat 130:47-56
Tan, Yu; Richards, Dylan; Coyle, Robert C et al. (2017) Cell number per spheroid and electrical conductivity of nanowires influence the function of silicon nanowired human cardiac spheroids. Acta Biomater 51:495-504
Chen, Wei; Zhang, Yong-Mei; Davies, Christopher (2017) Penicillin-Binding Protein 3 Is Essential for Growth of Pseudomonas aeruginosa. Antimicrob Agents Chemother 61:
Richards, Dylan; Jia, Jia; Yost, Michael et al. (2017) 3D Bioprinting for Vascularized Tissue Fabrication. Ann Biomed Eng 45:132-147
Ghatak, Shibnath; Hascall, Vincent C; Markwald, Roger R et al. (2017) Transforming growth factor ?1 (TGF?1)-induced CD44V6-NOX4 signaling in pathogenesis of idiopathic pulmonary fibrosis. J Biol Chem 292:10490-10519
Richards, Dylan J; Coyle, Robert C; Tan, Yu et al. (2017) Inspiration from heart development: Biomimetic development of functional human cardiac organoids. Biomaterials 142:112-123
Nolan, Krystal D; Kaur, Jasmine; Isaacs, Jennifer S (2017) Secreted heat shock protein 90 promotes prostate cancer stem cell heterogeneity. Oncotarget 8:19323-19341

Showing the most recent 10 out of 61 publications