The Vascular and Cardiac Function Core will provide services and training for COBRE and LSUHSC investigators who will use animal models of cardiovascular disease and/or in vivo technology to answer specific aims outlined in their projects.
The specific aims of this Core are: 1. To provide skilled personnel to assist and/or perform highly specialized surgical and technical procedures involving ultrasound imaging, radio telemetric recording of blood pressure, use of pressure-volume conductance catheters to assess cardiac structure and function, sympathetic nerve recording, microinjection procedures, vascular injury, cardiac ischemia and blood flow, and assessment of renal function;2. To assist in producing models of hypertension, ischemia/reperfusion injury, vascular injury, atherosclerosis, diabetes, cerebral ischemia, acute kidney injury, volume overload and drug-induced cardiac damage from which tissues will be collected for histological, biochemical, genetic and/or proteomic analysis;3. To conduct experiments and assist COBRE and LSUHSC investigators with the collection and interpretation of physiological data;4. To educate investigators regarding potential uses of The Vascular and Cardiac Function Core resources to enhance their research objectives and provide avenues towards developing translational research projects. Investigators will have access to the core facilities to complete experimental protocols on their own or with the aid and supervision of the Core's technical staff. The core will also provide services for the design and development of new animal models for cardiovascular research. The services ofthe Core encompass;1) ultrasound imaging, 2) measurement of blood pressure, heart rate, spontaneous baroreflex, and core body temperature by radio telemetry, 3) tail cuff analysis of blood pressure and heart rate, 4) cardiac left ventricular pressure-volume analysis, 5) CNS microinjection, 6) microdialysis, 7) nerve recording, 8) tissue collection, 9) blood flow analysis, 10) blood chemistry, and 11) assessment ofthe renal excretion of water and electrolytes. The state-of-the-art technology and experienced personnel provided by this Core will enhance the COBRE and University investigators progress towards acquisition of extramural funding.
With the exponentially increasing number of mouse and rat models of disease there is a dire need for reliable and flexible cardiovascular phenotyping cores. Investigators from diverse disciplines often lack the training necessary to determine the cardiovascular phenotype of mouse and rat models and rely more and more on core expertise to do so.
|Delgado-Peraza, Francheska; Ahn, Kwang H; Nogueras-Ortiz, Carlos et al. (2016) Mechanisms of Biased Î²-Arrestin-Mediated Signaling Downstream from the Cannabinoid 1 Receptor. Mol Pharmacol 89:618-29|
|Sanchez, Maria Dulfary; Ochoa, Augusto C; Foster, Timothy P (2016) Development and evaluation of a host-targeted antiviral that abrogates herpes simplex virus replication through modulation of arginine-associated metabolic pathways. Antiviral Res 132:13-25|
|Ibba, Salome' V; Ghonim, Mohamed A; Pyakurel, Kusma et al. (2016) Potential of Inducible Nitric Oxide Synthase as a Therapeutic Target for Allergen-Induced Airway Hyperresponsiveness: A Critical Connection to Nitric Oxide Levels and PARP Activity. Mediators Inflamm 2016:1984703|
|Chuang, Gin C; Xia, Huijing; Mahne, Sarah E et al. (2016) Environmentally Persistent Free Radicals Cause Apoptosis in HL-1 Cardiomyocytes. Cardiovasc Toxicol :|
|Burn, Brendan R; Varner, Kurt J (2015) Environmentally persistent free radicals compromise left ventricular function during ischemia/reperfusion injury. Am J Physiol Heart Circ Physiol 308:H998-H1006|
|Ghonim, Mohamed A; Pyakurel, Kusma; Ibba, Salome V et al. (2015) PARP is activated in human asthma and its inhibition by olaparib blocks house dust mite-induced disease in mice. Clin Sci (Lond) 129:951-62|
|Harrison-Bernard, Lisa M (2015) Sphingolipids, new kids on the block, promoting glomerular fibrosis in the diabetic kidney. Am J Physiol Renal Physiol 309:F685-6|
|Xia, Huijing; de Queiroz, Thyago Moreira; Sriramula, Srinivas et al. (2015) Brain ACE2 overexpression reduces DOCA-salt hypertension independently of endoplasmic reticulum stress. Am J Physiol Regul Integr Comp Physiol 308:R370-8|
|Sriramula, Srinivas; Xia, Huijing; Xu, Ping et al. (2015) Brain-targeted angiotensin-converting enzyme 2 overexpression attenuates neurogenic hypertension by inhibiting cyclooxygenase-mediated inflammation. Hypertension 65:577-86|
|Mendoza, Alberto; Lazartigues, Eric (2015) The compensatory renin-angiotensin system in the central regulation of arterial pressure: new avenues and new challenges. Ther Adv Cardiovasc Dis 9:201-8|
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