The CTDL was designed to provide a centralized service to support investigators in the College of Dentistry who required biologic measures fortheir clinical, translational, and basic science projects. The core support services in the Phase III center operations include: (1) an array of measures of host responses in a range of biologic fluids (eg. serum, saliva, cervico-vaginal fluids, cell culture supernatants, tissue homogenates, etc.); (2) microbiological/virological assays for oral bacteria and bacteria from other mucosal sites, and viruses related to oral diseases. This includes cultivation, molecular analysis, and preparation of batches of organisms for in vitro studies;(3) genetics/genomics analysis which include preparation of DNA and RNA from various samples, analytic approaches for GWAS, SNP, miRNA, and epigenetic variations related to specific project needs;and (4) mineralized tissue analysis including both histomorphometric, microCT, and cellular studies of bone biology.

Public Health Relevance

While the CTDL is primarily designed to support UK investigators for a range of analytic services, specimens evaluated in this core could also be derived from populations derived from community-based partners. In either case, these outcomes will help create new knowledge of the biology of oral-systemic disease relationships to improve oral health in the population.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Center Core Grants (P30)
Project #
1P30GM110788-01
Application #
8735428
Study Section
Special Emphasis Panel (ZGM1-TWD-C (C3))
Project Start
Project End
Budget Start
2014-08-15
Budget End
2015-07-31
Support Year
1
Fiscal Year
2014
Total Cost
$433,276
Indirect Cost
$144,505
Name
University of Kentucky
Department
Type
DUNS #
939017877
City
Lexington
State
KY
Country
United States
Zip Code
40506
Ma, Fei; Wang, Chunmei; Yoder, William E et al. (2016) Efficacy of Herpes Simplex Virus Vector Encoding the Human Preproenkephalin Gene for Treatment of Facial Pain in Mice. J Oral Facial Pain Headache 30:42-50
Gonzalez, O A; Nagarajan, R; Novak, M J et al. (2016) Immune system transcriptome in gingival tissues of young nonhuman primates. J Periodontal Res 51:152-63
Iglesias-Linares, Alejandro; Morford, Lorri Ann; Hartsfield Jr, James Kennedy (2016) Bone Density and Dental External Apical Root Resorption. Curr Osteoporos Rep 14:292-309
Nagarajan, Radhakrishnan; Al-Sabbagh, Mohanad; Dawson 3rd, Dolph et al. (2016) Integrated Biomarker Profiling of Smokers with Periodontitis. J Clin Periodontol :
Gonzalez, Octavio A; Orraca, Luis; Kensler, Terry B et al. (2016) Familial periodontal disease in the Cayo Santiago rhesus macaques. Am J Primatol 78:143-51
Pandruvada, Subramanya N; Gonzalez, Octavio A; Kirakodu, Sreenatha et al. (2016) Bone biology-related gingival transcriptome in ageing and periodontitis in non-human primates. J Clin Periodontol 43:408-17
Ebersole, J L; Kirakodu, S; Novak, M J et al. (2016) Effects of aging in the expression of NOD-like receptors and inflammasome-related genes in oral mucosa. Mol Oral Microbiol 31:18-32
Sharab, L Y; Morford, L A; Dempsey, J et al. (2015) Genetic and treatment-related risk factors associated with external apical root resorption (EARR) concurrent with orthodontia. Orthod Craniofac Res 18 Suppl 1:71-82
Bruno, Maria E C; Rogier, Eric W; Arsenescu, Razvan I et al. (2015) Correlation of Biomarker Expression in Colonic Mucosa with Disease Phenotype in Crohn's Disease and Ulcerative Colitis. Dig Dis Sci 60:2976-84
Eisenlohr-Moul, Tory A; Crofford, Leslie J; Howard, Thomas W et al. (2015) Parasympathetic reactivity in fibromyalgia and temporomandibular disorder: associations with sleep problems, symptom severity, and functional impairment. J Pain 16:247-57

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