Overall Objective and Rationale: The existing Neuroscience and Imaging Core has been reorganized and is now entitled "Cell Biology and Cellular Imaging." The aim is to provide expertise, services and equipment to broadly support cellular and molecular neuroscience research which, in our Center, is focused on elucidating the mechanisms of pathophysiology associated with the genetic and environmentally-induced developmental diseases affecting CNS development and function. We upgraded tissue processing for histology and cellular imaging with stateof-the art instruments like the Zeiss Laser Scanning Confocal Microscope LSM 510 META and the Leica Laser Cell Capture Microdissection system. We also equipped 3 new cell culture rooms to support our expanding needs for neural stem/progenitor cell culture methodologies to study glial and neuronal cell biology and for transplantation studies. The histology component has now been eliminated, as similar services are now available from the BRI histology core. Although a new and separate Stem Cell Core is proposed to focus on human embryonic stem cells (Core C), the Cell Biology and Cellular Imaging Core will continue to support cell culture of rodent and human brain cell subpopulations, and will expand the cellular imaging component. The demand for the latter has grown dramatically, becoming particular heavily used by faculty working with animal models of developmental diseases. We will also initiate a new subcomponent to provide expertise in the area of cellular immunology. This is done with the growing recognition that immune function and/or inflammatory responses impact a great number of IDD, and is a critical of component of investigation in human studies as well as our many animal models. This new function is included in this Core because it utilizes cell culture and cellular imaging as principle tools, although the methodologies in many cases are highly specialized, for example, the use of FACS to examine and investigate the cellular and molecular character of the immune response.

National Institute of Health (NIH)
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Center Core Grants (P30)
Project #
Application #
Study Section
Special Emphasis Panel (ZHD1-MRG-C)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of California Los Angeles
Los Angeles
United States
Zip Code
Cui, Yijun; Ostlund, Sean B; James, Alex S et al. (2014) Targeted expression of ?-opioid receptors in a subset of striatal direct-pathway neurons restores opiate reward. Nat Neurosci 17:254-61
Wang, Nan; Gray, Michelle; Lu, Xiao-Hong et al. (2014) Neuronal targets for reducing mutant huntingtin expression to ameliorate disease in a mouse model of Huntington's disease. Nat Med 20:536-41
Tsoa, Rosemarie W; Coskun, Volkan; Ho, Chi K et al. (2014) Spatiotemporally different origins of NG2 progenitors produce cortical interneurons versus glia in the mammalian forebrain. Proc Natl Acad Sci U S A 111:7444-9
Fogel, Brent L; Cho, Ellen; Wahnich, Amanda et al. (2014) Mutation of senataxin alters disease-specific transcriptional networks in patients with ataxia with oculomotor apraxia type 2. Hum Mol Genet 23:4758-69
Liu, Hailiang; Chen, Yongchang; Niu, Yuyu et al. (2014) TALEN-mediated gene mutagenesis in rhesus and cynomolgus monkeys. Cell Stem Cell 14:323-8
Waschek, J A (2013) VIP and PACAP: neuropeptide modulators of CNS inflammation, injury, and repair. Br J Pharmacol 169:512-23
Tan, Yossan-Var; Abad, Catalina; Wang, Yuqi et al. (2013) Pituitary adenylate cyclase activating peptide deficient mice exhibit impaired thymic and extrathymic regulatory T cell proliferation during EAE. PLoS One 8:e61200
Yan, Yan; Zhou, Xiaofeng; Pan, Zui et al. (2013) Pro- and anti-mitogenic actions of pituitary adenylate cyclase-activating polypeptide in developing cerebral cortex: potential mediation by developmental switch of PAC1 receptor mRNA isoforms. J Neurosci 33:3865-78
de Vellis, Jean; Cole, Ruth (2012) Preparation of mixed glial cultures from postnatal rat brain. Methods Mol Biol 814:49-59
Wu, Nanping; Joshi, Prasad R; Cepeda, Carlos et al. (2010) Alpha-synuclein overexpression in mice alters synaptic communication in the corticostriatal pathway. J Neurosci Res 88:1764-76

Showing the most recent 10 out of 23 publications