Dysfunction of complex I of the mitochondrial respiratory chain (RC) commonly causes multi-systemic diseases that can manifest at any age, often involving severe neurodevelopmental impairment, developmental regression, autism-spectrum behaviors, leukoencephalopathy, basal ganglia stroke, ataxia, and seizures. Cellular mechanisms that cause disease are not known, thereby hampering effective treatment attempts. Improved understanding of the cellular pathophysiology mediating disease in genetic-based RC dysfunction may lead to novel treatment options. Our preliminary data suggests that alterations of the PPAR/SIRT1 signaling pathway occur in animal models and human patients with RC dysfunction. We hypothesize that NAD+ deficiency and/or increased oxidant stress caused by RC complex I dysfunction inhibits PPAR/SIRT1 pathway signaling, which then orchestrates downstream deleterious effects across many aspects of intermediary metabolism.
Our specific aim i s to determine whether pharmacologic modulation of the PPAR/SIRT1 pathway will attenuate, or even reverse, the metabolic sequelae of complex I dysfunction. We will pursue this goal in a C. elegans model that harbors a missense mutation in a nuclear gene encoding a subunit of RC complex I (gas-1(fc21)) through study of 5 agents that directly modulate the PPAR/SIRT1 pathway. Therapeutic effects will be assessed by analysis of (a) Lifespan (primary endpoint); (b) Global metabolomic response reflected in 3 complementary assays: i) Microarray expression analysis of biochemical pathways, ii) Stable isotopic analysis of in vivo metabolic flux;iii) Quantitative PCR analysis of PPAR/SIRT1 pathway gene expression;and (c) Integrated mitochondrial functions assessed by fluorescence microscopy of in vivo mitochondria content, membrane potential, and matrix oxidant burden. We will further investigate phenotypic responses by in vivo functional analyses in gas-1(fc21) complex 1 mutants that harbor secondary knockdown by RNA interference for individual PPAR/SIRT1 pathway genes. The C. elegans models thus allows for low-cost and rapid evaluation of novel therapeutic targets for mitochondrial RC dysfunction, a form of human neurologic disease for which effective treatment remains elusive.
The ability to treat a devastating class of neurologic diseases that are caused by mitochondrial complex I dysfunction will be objectively studied in a simple C. elegans model animal through pharmacologic modulation of the highly conserved PPAR/SIRT1 signaling pathway that orchestrates metabolism.
|Carlson, G C; Lin, R E; Chen, Y et al. (2016) Dexras1 a unique ras-GTPase interacts with NMDA receptor activity and provides a novel dissociation between anxiety, working memory and sensory gating. Neuroscience 322:408-15|
|Chen, Yong; Bang, Sookhee; McMullen, Mary F et al. (2016) Neuronal Activity-Induced Sterol Regulatory Element Binding Protein-1 (SREBP1) is Disrupted in Dysbindin-Null Mice-Potential Link to Cognitive Impairment in Schizophrenia. Mol Neurobiol :|
|Ghosh, Mausam; Lane, Meredith; Krizman, Elizabeth et al. (2016) The transcription factor Pax6 contributes to the induction of GLT-1 expression in astrocytes through an interaction with a distal enhancer element. J Neurochem 136:262-75|
|Opladen, Thomas; Lindner, Martin; Das, Anibh M et al. (2016) In vivo monitoring of urea cycle activity with (13)C-acetate as a tracer of ureagenesis. Mol Genet Metab 117:19-26|
|Port, Russell G; Gaetz, William; Bloy, Luke et al. (2016) Exploring the relationship between cortical GABA concentrations, auditory gamma-band responses and development in ASD: Evidence for an altered maturational trajectory in ASD. Autism Res :|
|White, Rachel S; Bhattacharya, Anup K; Chen, Yong et al. (2016) Lysosomal iron modulates NMDA receptor-mediated excitation via small GTPase, Dexras1. Mol Brain 9:38|
|Edgar, J Christopher; Fisk 4th, Charles L; Liu, Song et al. (2016) Translating Adult Electrophysiology Findings to Younger Patient Populations: Difficulty Measuring 40-Hz Auditory Steady-State Responses in Typically Developing Children and Children with Autism Spectrum Disorder. Dev Neurosci 38:1-14|
|Port, Russell G; Edgar, J Christopher; Ku, Matthew et al. (2016) Maturation of auditory neural processes in autism spectrum disorder - A longitudinal MEG study. Neuroimage Clin 11:566-77|
|Antezana, Ligia; Mosner, Maya G; Troiani, Vanessa et al. (2016) Social-Emotional Inhibition of Return in Children with Autism Spectrum Disorder Versus Typical Development. J Autism Dev Disord 46:1236-46|
|Mlynarski, Elisabeth E; Xie, Michael; Taylor, Deanne et al. (2016) Rare copy number variants and congenital heart defects in the 22q11.2 deletion syndrome. Hum Genet 135:273-85|
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