E. Justification A key goal of this Core is to provide access to state-of-the-art imaging technologies to researchers at CN and GWU to perform studies on cells and animal models in this area of IDDR. In addition, the Cellular Imaging and Analysis Core assist investigators in their studies on a variety of children's diseases through the expertise of the staff and the affiliated users. The services offered include assistance in 1) preparation of samples and examination of the cells and tissues by light microscopy and EM (see projects* 1-6), 2) quantitative analysis of the data obtained for each project and 3) obtaining cells and tissue samples to carry out genomics/proteomics analysis (see project#6). For these services we make use of dedicated staff in the Core and also through the communal participation of the users with specific expertise (see section E.2, 3). Additionally, we work together with other Cores including, the Genomics and Proteomics and the Biostatistics and Informatics Cores. Use of the recently acquired laser capture microscope (see section E.6.) has enhanced our ability for targeted extraction of diseased cells and tissues from animal or patient materials further enhancing the analysis of these materials with the help of the Genomics and Proteomics Core. Additionally the services provided for establishing neural cell cultures and cell lines have been valuable for subsequent analysis of these purified populations of cells for quantitative analysis of their molecular profiles through genomic and proteomics analysis. In the coming years, we will increase the support our Core provides in carrying out live imaging and quantitative analysis of imaging data. To this end, we have a new Director with extensive and outstanding experience in both these aspects and have hired a new Core Manager who has background and several years of experience in performing quantitative image analysis. In addition, we have started working closely with the Biostatistics and Informatics Core to enable the users to appropriately design and carry out suitable statistical analysis of their data. We are also working in close collaboration with other IDDRCs nationwide - one example of which is the work that Drs. Corbin and Huntsman's have been performing in collaboration with the IDDRC at the Kennedy Krieger Institute at John's Hopkins Medical School. Their work with Dr. Walter Kaufmann (an expert clinician and scientist in the area of Fragile X Syndrome) makes use of the Cellular Imaging Core and has recently resulted in a publication on inhibitory synaptic defects in a mouse model of Fragile X Syndrome (Olmos-Serano et al., 2010).

National Institute of Health (NIH)
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Center Core Grants (P30)
Project #
Application #
Study Section
Special Emphasis Panel (ZHD1)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Children's Research Institute
United States
Zip Code
Sokolowski, Katie; Tran, Tuyen; Esumi, Shigeyuki et al. (2016) Molecular and behavioral profiling of Dbx1-derived neurons in the arcuate, lateral and ventromedial hypothalamic nuclei. Neural Dev 11:12
Glezer, Laurie S; Eden, Guinevere; Jiang, Xiong et al. (2016) Uncovering phonological and orthographic selectivity across the reading network using fMRI-RA. Neuroimage 138:248-56
Karpinski, Beverly A; A Bryan, Corey; Paronett, Elizabeth M et al. (2016) A cellular and molecular mosaic establishes growth and differentiation states for cranial sensory neurons. Dev Biol 415:228-41
You, Wonsang; Evangelou, Iordanis E; Zun, Zungho et al. (2016) Robust preprocessing for stimulus-based functional MRI of the moving fetus. J Med Imaging (Bellingham) 3:026001
Tasker, Robert C; Goodkin, Howard P; Sánchez Fernández, Iván et al. (2016) Refractory Status Epilepticus in Children: Intention to Treat With Continuous Infusions of Midazolam and Pentobarbital. Pediatr Crit Care Med 17:968-975
Mukhamedova, Nigora; Brichacek, Beda; Darwish, Christina et al. (2016) Analysis of ABCA1 and Cholesterol Efflux in HIV-Infected Cells. Methods Mol Biol 1354:281-92
Sreetama, S C; Takano, T; Nedergaard, M et al. (2016) Injured astrocytes are repaired by Synaptotagmin XI-regulated lysosome exocytosis. Cell Death Differ 23:596-607
Uittenbogaard, Martine; Chiaramello, Anne (2016) Novel subcellular localization of the DNA helicase Twinkle at the kinetochore complex during mitosis in neuronal-like progenitor cells. Histochem Cell Biol 145:275-86
Ratto, Allison B; Anthony, Bruno J; Kenworthy, Lauren et al. (2016) Are Non-intellectually Disabled Black Youth with ASD Less Impaired on Parent Report than Their White Peers? J Autism Dev Disord 46:773-81
Govindan, R B; Kota, Srinivas; Al-Shargabi, Tareq et al. (2016) Effect of electrocardiogram interference on cortico-cortical connectivity analysis and a possible solution. J Neurosci Methods 270:76-84

Showing the most recent 10 out of 295 publications