E. Justification A key goal of this Core is to provide access to state-of-the-art imaging technologies to researchers at CN and GWU to perform studies on cells and animal models in this area of IDDR. In addition, the Cellular Imaging and Analysis Core assist investigators in their studies on a variety of children's diseases through the expertise of the staff and the affiliated users. The services offered include assistance in 1) preparation of samples and examination of the cells and tissues by light microscopy and EM (see projects* 1-6), 2) quantitative analysis of the data obtained for each project and 3) obtaining cells and tissue samples to carry out genomics/proteomics analysis (see project#6). For these services we make use of dedicated staff in the Core and also through the communal participation of the users with specific expertise (see section E.2, 3). Additionally, we work together with other Cores including, the Genomics and Proteomics and the Biostatistics and Informatics Cores. Use of the recently acquired laser capture microscope (see section E.6.) has enhanced our ability for targeted extraction of diseased cells and tissues from animal or patient materials further enhancing the analysis of these materials with the help of the Genomics and Proteomics Core. Additionally the services provided for establishing neural cell cultures and cell lines have been valuable for subsequent analysis of these purified populations of cells for quantitative analysis of their molecular profiles through genomic and proteomics analysis. In the coming years, we will increase the support our Core provides in carrying out live imaging and quantitative analysis of imaging data. To this end, we have a new Director with extensive and outstanding experience in both these aspects and have hired a new Core Manager who has background and several years of experience in performing quantitative image analysis. In addition, we have started working closely with the Biostatistics and Informatics Core to enable the users to appropriately design and carry out suitable statistical analysis of their data. We are also working in close collaboration with other IDDRCs nationwide - one example of which is the work that Drs. Corbin and Huntsman's have been performing in collaboration with the IDDRC at the Kennedy Krieger Institute at John's Hopkins Medical School. Their work with Dr. Walter Kaufmann (an expert clinician and scientist in the area of Fragile X Syndrome) makes use of the Cellular Imaging Core and has recently resulted in a publication on inhibitory synaptic defects in a mouse model of Fragile X Syndrome (Olmos-Serano et al., 2010).

National Institute of Health (NIH)
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Center Core Grants (P30)
Project #
Application #
Study Section
Special Emphasis Panel (ZHD1)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Children's Research Institute
United States
Zip Code
Morton, Paul D; Korotcova, Ludmila; Lewis, Bobbi K et al. (2017) Abnormal neurogenesis and cortical growth in congenital heart disease. Sci Transl Med 9:
Sanz, Jacqueline H; Berl, Madison M; Armour, Anna C et al. (2017) Prevalence and pattern of executive dysfunction in school age children with congenital heart disease. Congenit Heart Dis 12:202-209
Salka, Kyle; Bhuvanendran, Shivaprasad; Wilson, Kassandra et al. (2017) Superresolution Imaging Identifies That Conventional Trafficking Pathways Are Not Essential for Endoplasmic Reticulum to Outer Mitochondrial Membrane Protein Transport. Sci Rep 7:16
Forbes, Thomas A; Gallo, Vittorio (2017) All Wrapped Up: Environmental Effects on Myelination. Trends Neurosci 40:572-587
Defour, Aurelia; Medikayala, Sushma; Van der Meulen, Jack H et al. (2017) Annexin A2 links poor myofiber repair with inflammation and adipogenic replacement of the injured muscle. Hum Mol Genet 26:1979-1991
Vila, Maria C; Rayavarapu, Sree; Hogarth, Marshall W et al. (2017) Mitochondria mediate cell membrane repair and contribute to Duchenne muscular dystrophy. Cell Death Differ 24:330-342
Moler, Frank W; Silverstein, Faye S; Holubkov, Richard et al. (2017) Therapeutic Hypothermia after In-Hospital Cardiac Arrest in Children. N Engl J Med 376:318-329
Tague, Lauren; Donofrio, Mary T; Fulgium, Amanda et al. (2017) Common Findings in Late-Gestation Fetal Echocardiography. J Ultrasound Med 36:2431-2437
Al-Shargabi, T; Govindan, R B; Dave, R et al. (2017) Inflammatory cytokine response and reduced heart rate variability in newborns with hypoxic-ischemic encephalopathy. J Perinatol 37:668-672
Lischinsky, Julieta E; Sokolowski, Katie; Li, Peijun et al. (2017) Embryonic transcription factor expression in mice predicts medial amygdala neuronal identity and sex-specific responses to innate behavioral cues. Elife 6:

Showing the most recent 10 out of 324 publications