The Genomics/Proteomics Core (GPC) of the IDDRC at Children's National Medical Center (Children's National, CN) has been highly active, with over 7,000 samples processed during the last award period and support for more than 40 investigators. Two key aspects of the success of the Core include innovation (new equipment and assays offered each year), and full service (including assistance with experimental design, sample processing, technical support and training, bioinformatics assistance, and help with grants and manuscripts). New technologies and services that will be implemented during the proposed new award period include emulsion PCR (RainDance custom panels), epigenomics scans (both Illumina and Pacific Biosciences single molecule whole genome), nextgen sequencing (Illumina and Pacific Biosciences), glycoproteomics, and phosphoproteomic scans. Objectives of the Core are: 1. Supply full service and innovative genomic and proteomic research tools to IDDRC investigators from project planning to data interpretation and manuscript writing with no service fees (users pay only for the cost of reagents). 2. Rapid acquisition and implementation of cutting-edge high-content technology. 3. Support of multi-scale projects inclusive of DNA, mRNA, microRNA, epigenomics, proteomics, and integrative and systems biology bioinformatics approaches. 4. Offer group and individualized training to increase Core capacity and provide flexibility to investigators. 5. Leverage Core support to users through synergism and integration of commensurate NIH- and philanthropy-supported genomics/proteomics/bioinformatics Cores: NICHD's National Center for Medical Rehabilitation Research (NCMRR-DC) Core for Molecular &Functional Outcome Measures in Rehabilitation Medicine, the CTSI-CN Genomics/Proteomics Core for Children's Translational Medicine, and the Sheikh Zayad Institute for Surgical Innovation. These synergisms enable introduction of advanced new and costly equipment into the IDDRC Genomics/Proteomics Core, and offer unique and substantial opportunities for resource sharing and collaboration for IDDRC investigators. In the previous Summary Statements of the 2006 review, the Genomics and Proteomics Core (previously called MGPAC) received an outstanding evaluation. The previous reviewers felt that bioinformatics support could be bolstered further, and better integrated with the Statistical Core. Towards this end, we have added two faculties to the Genomics/Proteomics Core personnel: statistical geneticist Dr. Gordish-Dressman and biomedical engineer and bioinformatics specialist Dr. Wang, and have ongoing meetings with the Statistical Core to integrate efforts. We have also submitted grants together with bioinformatics researchers at Georgetown University and Virginia Tech to continue our methods development work in bioinformatics support. Our Core experienced considerable expansion in the number of samples and complexity of user projects over the last funding cycle. A formal survey of anticipated user needs showed emerging strong demand for epigenomics, miRNA expression profiling, ELISA bead assays, next-generation sequencing, quantitative proteomics using stable isotope labeling and label free strategies, phosphoproteomics and glycoproteomics. To meet these needs, we significantly increased service offerings, implemented new equipment and new expertise. Our emphasis remains on offering highly specialized, innovative, full service support for a broad spectrum of genomic, proteomic and bioinformatic technologies.

National Institute of Health (NIH)
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Center Core Grants (P30)
Project #
Application #
Study Section
Special Emphasis Panel (ZHD1)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Children's Research Institute
United States
Zip Code
Morton, Paul D; Korotcova, Ludmila; Lewis, Bobbi K et al. (2017) Abnormal neurogenesis and cortical growth in congenital heart disease. Sci Transl Med 9:
Sanz, Jacqueline H; Berl, Madison M; Armour, Anna C et al. (2017) Prevalence and pattern of executive dysfunction in school age children with congenital heart disease. Congenit Heart Dis 12:202-209
Salka, Kyle; Bhuvanendran, Shivaprasad; Wilson, Kassandra et al. (2017) Superresolution Imaging Identifies That Conventional Trafficking Pathways Are Not Essential for Endoplasmic Reticulum to Outer Mitochondrial Membrane Protein Transport. Sci Rep 7:16
Forbes, Thomas A; Gallo, Vittorio (2017) All Wrapped Up: Environmental Effects on Myelination. Trends Neurosci 40:572-587
Defour, Aurelia; Medikayala, Sushma; Van der Meulen, Jack H et al. (2017) Annexin A2 links poor myofiber repair with inflammation and adipogenic replacement of the injured muscle. Hum Mol Genet 26:1979-1991
Vila, Maria C; Rayavarapu, Sree; Hogarth, Marshall W et al. (2017) Mitochondria mediate cell membrane repair and contribute to Duchenne muscular dystrophy. Cell Death Differ 24:330-342
Moler, Frank W; Silverstein, Faye S; Holubkov, Richard et al. (2017) Therapeutic Hypothermia after In-Hospital Cardiac Arrest in Children. N Engl J Med 376:318-329
Tague, Lauren; Donofrio, Mary T; Fulgium, Amanda et al. (2017) Common Findings in Late-Gestation Fetal Echocardiography. J Ultrasound Med 36:2431-2437
Al-Shargabi, T; Govindan, R B; Dave, R et al. (2017) Inflammatory cytokine response and reduced heart rate variability in newborns with hypoxic-ischemic encephalopathy. J Perinatol 37:668-672
Lischinsky, Julieta E; Sokolowski, Katie; Li, Peijun et al. (2017) Embryonic transcription factor expression in mice predicts medial amygdala neuronal identity and sex-specific responses to innate behavioral cues. Elife 6:

Showing the most recent 10 out of 324 publications