BASIC SCIENCE I CORE: CELL CULTURE/NEUROTROPIC VIRUS, NEUROSCIENCE, AND PROTEOMICS The overall objective of the Basic Science Core I (BSCI) is to provide in vitro shared resources and training to basic and clinical researchers to study neurological disorders at the molecular level, related to Human Immunodeficiency Virus (HIV) infection. During HIV central nervous system (CNS) disease, disparities between virus-associated insults and host-mediated attempts at rescue and repair are responsible for cognitive deficits. To protect cells of the CNS, and to promote beneficial responses, key elements in the signaling crosstalk between cells of the CNS and virus must be understood. Information regarding cell-virus biochemical signaling interactions must be addressed, in large part, by in vitro studies. The BSCI will provide investigators with reliable access to high quality CNS cell cultures, virus and viral products, and other factors that contribute to HIV CNS disease which are critical to delineate signaling pathways important in disease progression. BSCI will also provide assistance and expertise in classical neuroscience techniques and tools to support investigation of cellular/molecular interactions between cells of the CNS and HIV that underlie neurocognitive dysfunction. The BSCI will also provide state-of-the-art services for discovering the molecular mechanisms involved in the development of AIDS-associated CNS dysfunction through proteomics and data analysis. Novel methods for discovering of biomarkers, innovative differential expression profiling and bioinformatics along with interactomics and phosphoproteomics are among the services that will be reliably and efficiently offered to the investigators. Our core will work closely with the other cores to promote a comprehensive multidisciplinary collaborative center program. This synergistic approach will ensure the success of both recipients of CNACC developmental awards and other members of the Center in conducting productive high impact research into HIV associated neurological disorders.

Public Health Relevance

This core will provide cell culture and virology services to investigators performing biomedical research on AIDS and the nervous system. Moreover, in the context of this core facility, neuroAIDS researchers will be able to analyze clinical samples, cell culture, or animal models for biomarkers of disease and to uncover mechansims involved in the development and progression of CNS disorders associated with HIV-1 infection.

Agency
National Institute of Health (NIH)
Institute
National Institute of Mental Health (NIMH)
Type
Center Core Grants (P30)
Project #
5P30MH092177-02
Application #
8381113
Study Section
Special Emphasis Panel (ZMH1-ERB-F)
Project Start
Project End
Budget Start
2012-06-01
Budget End
2013-05-31
Support Year
2
Fiscal Year
2012
Total Cost
$333,711
Indirect Cost
$115,600
Name
Temple University
Department
Type
DUNS #
057123192
City
Philadelphia
State
PA
Country
United States
Zip Code
19122
Antell, Gregory C; Dampier, Will; Aiamkitsumrit, Benjamas et al. (2017) Evidence of Divergent Amino Acid Usage in Comparative Analyses of R5- and X4-Associated HIV-1 Vpr Sequences. Int J Genomics 2017:4081585
Dampier, Will; Antell, Gregory C; Aiamkitsumrit, Benjamas et al. (2017) Specific amino acids in HIV-1 Vpr are significantly associated with differences in patient neurocognitive status. J Neurovirol 23:113-124
Sariyer, Rahsan; De-Simone, Francesca I; Donadoni, Martina et al. (2017) Alcohol-Mediated Missplicing of Mcl-1 Pre-mRNA is Involved in Neurotoxicity. Alcohol Clin Exp Res 41:1715-1724
Tahrir, Farzaneh G; Knezevic, Tijana; Gupta, Manish K et al. (2017) Evidence for the Role of BAG3 in Mitochondrial Quality Control in Cardiomyocytes. J Cell Physiol 232:797-805
Dampier, Will; Sullivan, Neil T; Chung, Cheng-Han et al. (2017) Designing broad-spectrum anti-HIV-1 gRNAs to target patient-derived variants. Sci Rep 7:14413
Sami Saribas, A; Cicalese, Stephanie; Ahooyi, Taha Mohseni et al. (2017) HIV-1 Nef is released in extracellular vesicles derived from astrocytes: evidence for Nef-mediated neurotoxicity. Cell Death Dis 8:e2542
Gupta, Manish K; Kaminski, Rafal; Mullen, Brian et al. (2017) HIV-1 Nef-induced cardiotoxicity through dysregulation of autophagy. Sci Rep 7:8572
Ahooyi, Taha Mohseni; Shekarabi, Masoud; Decoppet, Emilie A et al. (2017) Network Analysis of Hippocampal Neurons by Microelectrode Array in the Presence of HIV-1 Tat and Cocaine. J Cell Physiol :
O'Connor, E E; Jaillard, A; Renard, F et al. (2017) Reliability of White Matter Microstructural Changes in HIV Infection: Meta-Analysis and Confirmation. AJNR Am J Neuroradiol 38:1510-1519
Gannon, Patrick J; Akay-Espinoza, Cagla; Yee, Alan C et al. (2017) HIV Protease Inhibitors Alter Amyloid Precursor Protein Processing via ?-Site Amyloid Precursor Protein Cleaving Enzyme-1 Translational Up-Regulation. Am J Pathol 187:91-109

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