C2 Core B;Gene-Targeting/Viral Vector Core C2.1 Rationale. Active genetic manipulation of mice and viral targeting of protein expression in neural tissue have resulted in a revolution in the study of the nervous system. Although interpretation of experiments involving genetic manipulation of mice must be performed with care, the ability to monitor changes in the CNS in vivo upon modification of a single gene makes this technique an indispensable tool for modern inquiry in neurobiology. The usefulness of genetically altered mice will grow even further with increased use of genetargeted mice expressing proteins deficient for specific protein-protein interactions, conditional knockouts, BAC-transgenics and animals expressing externally-triggered neuronal switches. Use of viral vectors to acutely alter protein expression is a powerful technique complementary to genetic manipulation. The group of NINDS researchers at the UCD AMC is using a variety of genetically altered mice and viral vectors to study fundamental questions in neurobiology. Although we have access to a the transgenic facility of the Charles C Gates Regenerative Medicine and Stem Cell Biology Program to produce gene targeted mice, the point of entry is a finished transgenic vector or transfected ES cells (see Appendix, letter from Dr. Peter Koch). Moreover, if Core B did not exist ES clone screening by PCR and/or Southern blotting - all labor intensive procedures - would have to be performed by the individual investigator. For transgenic mice, several lines are obtained for each construct injected. These lines then need to be tested initially to see if they have integrated the exogenous DNA. Subsequently, analysis of appropriate tissue-specific transgene expression is necessary, as well as the analysis of germ-line transmission. These ES and transgenic studies all require skills in molecular biology and mouse husbandry that are not the areas of expertise of the majority of investigators. Likewise, construction and production of viral vectors also requires specific expertise that is not easily established in most laboratories individually. Core B provides these specialized services to RMNDC users.

National Institute of Health (NIH)
National Institute of Neurological Disorders and Stroke (NINDS)
Center Core Grants (P30)
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National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
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University of Colorado Denver
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Thomas, Ajay X; Cruz Del Angel, Yasmin; Gonzalez, Marco I et al. (2016) Rapid Increases in proBDNF after Pilocarpine-Induced Status Epilepticus in Mice Are Associated with Reduced proBDNF Cleavage Machinery. eNeuro 3:
Zhuang, Yonghua; Berens-Norman, Heather M; Leser, J Smith et al. (2016) Mitochondrial p53 Contributes to Reovirus-Induced Neuronal Apoptosis and Central Nervous System Injury in a Mouse Model of Viral Encephalitis. J Virol 90:7684-91
Frank, Guido K W; Collier, Shaleise; Shott, Megan E et al. (2016) Prediction error and somatosensory insula activation in women recovered from anorexia nervosa. J Psychiatry Neurosci 41:304-11
Kumar, Maneesh G; Rowley, Shane; Fulton, Ruth et al. (2016) Altered Glycolysis and Mitochondrial Respiration in a Zebrafish Model of Dravet Syndrome. eNeuro 3:
Ferber, Alexander T; Benichoux, Victor; Tollin, Daniel J (2016) Test-Retest Reliability of the Binaural Interaction Component of the Auditory Brainstem Response. Ear Hear 37:e291-301
Dondzillo, Anna; Thompson, John A; Klug, Achim (2016) Recurrent Inhibition to the Medial Nucleus of the Trapezoid Body in the Mongolian Gerbil (Meriones Unguiculatus). PLoS One 11:e0160241
Lintz, Mario J; Felsen, Gidon (2016) Basal ganglia output reflects internally-specified movements. Elife 5:
Weitzel, Lindsay-Rae; Sampath, Dayalan; Shimizu, Kaori et al. (2016) EEG power as a biomarker to predict the outcome after cardiac arrest and cardiopulmonary resuscitation induced global ischemia. Life Sci 165:21-25
Mathews, Emily S; Appel, Bruce (2016) Cholesterol Biosynthesis Supports Myelin Gene Expression and Axon Ensheathment through Modulation of P13K/Akt/mTor Signaling. J Neurosci 36:7628-39
Heischmann, Svenja; Quinn, Kevin; Cruickshank-Quinn, Charmion et al. (2016) Exploratory Metabolomics Profiling in the Kainic Acid Rat Model Reveals Depletion of 25-Hydroxyvitamin D3 during Epileptogenesis. Sci Rep 6:31424

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