Core B Nanoscopy Super-resolution light microscopy or nanoscopy (i.e. microscopy with nanometer-scale resolution) is defined as imaging with a resolution below the diffraction limit of conventional light microscopy, which is ~200 nm in the equatorial (xy) and ~500 nm in the axial (z) dimension. Recently developed super-resolution methods that allow light microscopy to image subcellular structure and molecular localization on the 10s of nm scale have revolutionized neurobiology by allowing neuroscientists to study the inner workings of glia, axons, dendrites, and synapses in unprecedented detail that had previously only been possible using electron microscopy (EM). In particular, super-resolution fluorescence imaging has many advantages over EM including much easier sample preparation and staining procedures as well as the ability to be applied to not only fixed, but also living cells and tissues. Several different super-resolution fluorescence imaging methods have been developed, such as STimulated-Emission Depletion (STED) microscopy and the related methods of Stochastic Optical Reconstruction Microscopy (STORM) and Photo-activation Localization Microscopy (PALM). Each of these nanoscopy methods has advantages and limitations depending on the properties of the biological sample and fluorophores to be imaged. Thus, it is advantageous and even necessary to employ more than one of these different methods when investigating any given scientific question. Accordingly, the RMNDC Nanoscopy Core B will provide access to recently acquired instrumentation and technical support for NINDS-funded and other neuroscience investigators at the University of Colorado-Anschutz Medical Campus (UC-AMC) to perform state-of-the-art STED and STORM/PALM super-resolution imaging (Aim 1) and complementary Forster-resonance energy transfer (FRET)-based imaging (Aim 2).

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Center Core Grants (P30)
Project #
5P30NS048154-14
Application #
9606100
Study Section
Special Emphasis Panel (ZNS1)
Project Start
Project End
Budget Start
2019-01-01
Budget End
2019-12-31
Support Year
14
Fiscal Year
2019
Total Cost
Indirect Cost
Name
University of Colorado Denver
Department
Type
DUNS #
041096314
City
Aurora
State
CO
Country
United States
Zip Code
80045
Purkey, Alicia M; Woolfrey, Kevin M; Crosby, Kevin C et al. (2018) AKAP150 Palmitoylation Regulates Synaptic Incorporation of Ca2+-Permeable AMPA Receptors to Control LTP. Cell Rep 25:974-987.e4
Cook, Sarah G; Bourke, Ashley M; O'Leary, Heather et al. (2018) Analysis of the CaMKII? and ? splice-variant distribution among brain regions reveals isoform-specific differences in holoenzyme formation. Sci Rep 8:5448
Tracy, Matthew E; Tesic, Vesna; Stamenic, Tamara Timic et al. (2018) CaV3.1 isoform of T-type calcium channels supports excitability of rat and mouse ventral tegmental area neurons. Neuropharmacology 135:343-354
Williams, Jason S; Hsu, Jessica Y; Rossi, Christy Cortez et al. (2018) Requirement of zebrafish pcdh10a and pcdh10b in melanocyte precursor migration. Dev Biol :
Frank, Guido K W; DeGuzman, Marisa C; Shott, Megan E et al. (2018) Association of Brain Reward Learning Response With Harm Avoidance, Weight Gain, and Hypothalamic Effective Connectivity in Adolescent Anorexia Nervosa. JAMA Psychiatry 75:1071-1080
Peña, Brisa; Laughter, Melissa; Jett, Susan et al. (2018) Injectable Hydrogels for Cardiac Tissue Engineering. Macromol Biosci 18:e1800079
Olsavsky, Aviva K; Shott, Megan E; DeGuzman, Marisa C et al. (2018) Neural correlates of taste reward value across eating disorders. Psychiatry Res Neuroimaging :
Ramirez-Gordillo, Daniel; Ma, Ming; Restrepo, Diego (2018) Precision of Classification of Odorant Value by the Power of Olfactory Bulb Oscillations Is Altered by Optogenetic Silencing of Local Adrenergic Innervation. Front Cell Neurosci 12:48
Gano, Lindsey B; Liang, Li-Ping; Ryan, Kristen et al. (2018) Altered mitochondrial acetylation profiles in a kainic acid model of temporal lobe epilepsy. Free Radic Biol Med 123:116-124
Moreno, Rosa L; Williams, Kristina; Jones, Kenneth L et al. (2018) Investigation of Islet2a function in zebrafish embryos: Mutants and morphants differ in morphologic phenotypes and gene expression. PLoS One 13:e0199233

Showing the most recent 10 out of 142 publications