Neurophysiologic measures of brain electrical activity, oxygenation state, temperature, and blood perfusion critically reflect brain function and health and provide important mechanistic insight for MR studies. Appropriate utilization of these neurophysiologic measures in conjunction with MR requires substantial expertise with suitable physiological monitoring and control of animals. The overall goal of Core 3 is to support integration of state-of-the-art neurophysiological measurements with MR studies in NINDS and related NIH-supported clinical and basic neuroscience research. In the last cycle, we achieved this aim by developing and implementing a wide array of optical and electrical measurement methods including extracellular recordings (single microelectrodes, electrode arrays), tissue oxygenation (pO2) with phosphorescence quenching, temperature monitoring with thermocouple wires, and blood flow with laser Doppler flowmetry. The qualifying user group of Core 3 consists of 7 Pis funded by NINDS who along with 11 other NIH-funded Pis use multimodal neurophysiology and other cross-modal methods to address fundamental questions in basic and clinical neuroscience. The impact of Core 3 on the Yale neuroscience community is demonstrated by the support it provided to 15 NIH grants and 7 new research initiatives that led to grants and papers, the training in advanced neurophysiology methods provided to 5 young neuroscientists (3 in NINDS PI laboratories), and the use of Core resources in 33 papers that led to 355 citations (for Core 3 alone this represents 30% of the total citations for 106 papers for all Cores). For the next cycle, we will continue to implement, maintain, and support innovative multi-modal neurophysiology methods for Core 3 users, support new research initiatives, train and provide mentorship for neuroscience Pis and their personnel for Core 3 usage, integrate synergistic use of MR/neurophysiological measurements and project-specific data analysis, implement new Core 3 methods to support neuroscience Pis, and track Core 3 activities and disseminate/share resources to NIH community. The newly implemented applications will include utilizing multi-electrode arrays and laser speckle methods to support cross-disciplinary MR studies.

Public Health Relevance

for Core 3 Neurophysiologic measures of brain electrical activity, oxygenation state, temperature, and blood perfusion critically reflect brain function and health and complement magnetic resonance (MR) studies. Appropriate utilization of these neurophysiologic measures in conjunction with MR requires substantial expertise with suitable physiological monitoring and control of animals. Core 3 will support integration of state-of-the-art neurophysiology to advance NIH-supported projects of direct relevance to public health.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Center Core Grants (P30)
Project #
2P30NS052519-06A1
Application #
8732258
Study Section
Special Emphasis Panel (ZNS1-SRB-B (38))
Project Start
Project End
Budget Start
2014-01-01
Budget End
2014-12-31
Support Year
6
Fiscal Year
2014
Total Cost
$161,596
Indirect Cost
$54,357
Name
Yale University
Department
Type
DUNS #
043207562
City
New Haven
State
CT
Country
United States
Zip Code
06520
Kaneko, Gen; Sanganahalli, Basavaraju G; Groman, Stephanie M et al. (2017) Hypofrontality and Posterior Hyperactivity in Early Schizophrenia: Imaging and Behavior in a Preclinical Model. Biol Psychiatry 81:503-513
Chowdhury, Golam M I; Wang, Peili; Ciardi, Alisha et al. (2017) Impaired Glutamatergic Neurotransmission in the Ventromedial Hypothalamus May Contribute to Defective Counterregulation in Recurrently Hypoglycemic Rats. Diabetes 66:1979-1989
Wang, Helen; Huang, Yuegao; Coman, Daniel et al. (2017) Network evolution in mesial temporal lobe epilepsy revealed by diffusion tensor imaging. Epilepsia 58:824-834
Thompson, Garth J (2017) Neural and metabolic basis of dynamic resting state fMRI. Neuroimage :
Tricò, Domenico; Prinsen, Hetty; Giannini, Cosimo et al. (2017) Elevated ?-Hydroxybutyrate and Branched-Chain Amino Acid Levels Predict Deterioration of Glycemic Control in Adolescents. J Clin Endocrinol Metab 102:2473-2481
Kundishora, Adam J; Gummadavelli, Abhijeet; Ma, Chanthia et al. (2017) Restoring Conscious Arousal During Focal Limbic Seizures with Deep Brain Stimulation. Cereb Cortex 27:1964-1975
Chowdhury, G M I; Zhang, J; Thomas, M et al. (2017) Transiently increased glutamate cycling in rat PFC is associated with rapid onset of antidepressant-like effects. Mol Psychiatry 22:120-126
Feng, Li; Motelow, Joshua E; Ma, Chanthia et al. (2017) Seizures and Sleep in the Thalamus: Focal Limbic Seizures Show Divergent Activity Patterns in Different Thalamic Nuclei. J Neurosci 37:11441-11454
Rao, Jyotsna U; Coman, Daniel; Walsh, John J et al. (2017) Temozolomide arrests glioma growth and normalizes intratumoral extracellular pH. Sci Rep 7:7865
Prinsen, Hetty; de Graaf, Robin A; Mason, Graeme F et al. (2017) Reproducibility measurement of glutathione, GABA, and glutamate: Towards in vivo neurochemical profiling of multiple sclerosis with MR spectroscopy at 7T. J Magn Reson Imaging 45:187-198

Showing the most recent 10 out of 136 publications