(BFNL) As one of the three Stanford Neuroscience Cores, the Stanford Behavioral and Functional Neuroscience Laboratory (BFNL). This core facility provides a centralized resource for standard and automated sensorimotor, learning and memory, and social behavioral paradigms as well as expertise in rodent models of CNS disorders in conjunction to pharmacological and experimental drug testing. In addition, automated behavior testing paradigms established by this core have helped our community to further address the standardization and reproducibility of behavioral tests using state-of-the-art apparatuses such as Intellicage and PhenoLab automated home environment monitoring systems. These systems provide complete home- cage sensorimotor behavioral monitoring capabilities and can test simple and complex learning and memory tasks in a fully automated fashion with minimal human intervention. Using this expertise and technology BFNL has provided state-of-the-art phenotyping services for novel transgenic lines and provided validated CNS Disease Models for testing novel compounds and neuropharmacological exploration during the past several years. BFNL has freely shared experimental protocols and data. The detailed list of behavioral paradigms, standard operating procedures, protocols, and a sample data set for most behavioral paradigm has been shared publicly at http://sbfnl.stanford.edu/testingtable. Over the past four years, BFNL has supported 55 labs and faculty with almost 40,000 hours of services. This effort has led to more than 20 publications overall. Going forward BFNL will continue providing these services and allocate additional resources to address more actively some of the rising concerns in the field about the predictive value and inherent limitations of animal models in drug discovery and also innovate and expand upon its automated testing capabilities. All of these advances will be shared with the neuroscience community via our newly improved resource sharing websites.

Public Health Relevance

(BFNL) The essential function of the Stanford Behavioral and Functional Neuroscience Laboratory (BFNL) is to provide a shared resource platform for academic labs, providing access to rodent behavior testing in existing and novel paradigms for characterizing behavior. This core facility provides a centralized resource for standard and automated sensorimotor, learning and memory, and social behavioral paradigms as well as expertise in rodent models of CNS disorders in conjunction to pharmacological and experimental drug testing.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Center Core Grants (P30)
Project #
5P30NS069375-08
Application #
9380981
Study Section
Special Emphasis Panel (ZNS1)
Project Start
Project End
Budget Start
2017-12-01
Budget End
2018-11-30
Support Year
8
Fiscal Year
2018
Total Cost
Indirect Cost
Name
Stanford University
Department
Type
DUNS #
009214214
City
Stanford
State
CA
Country
United States
Zip Code
94304
Szabo, Gergely G; Du, Xi; Oijala, Mikko et al. (2017) Extended Interneuronal Network of the Dentate Gyrus. Cell Rep 20:1262-1268
Liu, Qing; Jiang, Chao; Xu, Jin et al. (2017) Genome-Wide Temporal Profiling of Transcriptome and Open Chromatin of Early Cardiomyocyte Differentiation Derived From hiPSCs and hESCs. Circ Res 121:376-391
Harterink, Martin; da Silva, Marta Esteves; Will, Lena et al. (2017) DeActs: genetically encoded tools for perturbing the actin cytoskeleton in single cells. Nat Methods 14:479-482
Becker, Lindsay A; Huang, Brenda; Bieri, Gregor et al. (2017) Therapeutic reduction of ataxin-2 extends lifespan and reduces pathology in TDP-43 mice. Nature 544:367-371
Ardestani, Pooneh Memar; Evans, Andrew K; Yi, Bitna et al. (2017) Modulation of neuroinflammation and pathology in the 5XFAD mouse model of Alzheimer's disease using a biased and selective beta-1 adrenergic receptor partial agonist. Neuropharmacology 116:371-386
Si, Peng; Sen, Debasish; Dutta, Rebecca et al. (2017) In Vivo Molecular Optical Coherence Tomography of Lymphatic Vessel Endothelial Hyaluronan Receptors. Sci Rep 7:1086
Liba, Orly; Lew, Matthew D; SoRelle, Elliott D et al. (2017) Speckle-modulating optical coherence tomography in living mice and humans. Nat Commun 8:15845
Yi, Bitna; Sahn, James J; Ardestani, Pooneh Memar et al. (2017) Small molecule modulator of sigma 2 receptor is neuroprotective and reduces cognitive deficits and neuroinflammation in experimental models of Alzheimer's disease. J Neurochem 140:561-575
Chamberland, Simon; Yang, Helen H; Pan, Michael M et al. (2017) Fast two-photon imaging of subcellular voltage dynamics in neuronal tissue with genetically encoded indicators. Elife 6:
Gulati, Srishti; Cao, Vania Y; Otte, Stephani (2017) Multi-layer Cortical Ca2+ Imaging in Freely Moving Mice with Prism Probes and Miniaturized Fluorescence Microscopy. J Vis Exp :

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