The Drosophila Genomics Resource Center (DGRC) supports the international community of scientists utilizing Drosophila melanogaster for biomedical research. The mission of the DGRC is to 1) provide broad access to genomics resources by acquiring, archiving, curating, and distributing genomics resources including clones, vectors, and cell lines; 2) facilitate effective use of these genomics resources by providing guidance and support; and 3) improve the genomics resources and protocols available for Drosophila research. By preserving vital research materials and distributing them efficiently, the DGRC assures economical access and enhances scientific rigor and reproducibility.
The first aim of this proposal is to continue and strengthen the successful DGRC programs for acquiring, distributing genomics resources and facilitating their effective use. This will include augmenting and updating data management systems, the web interface and user support, as well as continuing the effective cost recovery program. The goal is to maximize the long-term viability of the DGRC and its benefits to users and the NIH.
The second aim i s to increase the utility of Drosophila as a model system by generating new resources through four projects: 1) surveying the transfection and CRISPR/Cas9 efficiency of gene tagging across modENCODE cell lines, 2) generating a universal CRISPR/Cas9 based transfection toolkit for the insertion of constructs and the subsequent ability to generate stable transformants in any Drosophila cell line, 3) establishing a neuroblast cell line as a model for stem cell biology, and 4) creating new metabolic sensors for characterizing physiological processes in cell lines and Drosophila tissues.

Project Start
Project End
Budget Start
2019-04-01
Budget End
2020-03-31
Support Year
16
Fiscal Year
2019
Total Cost
Indirect Cost
Name
Indiana University Bloomington
Department
Type
DUNS #
006046700
City
Bloomington
State
IN
Country
United States
Zip Code
47401
Neuman, Sarah D; Bashirullah, Arash (2018) Hobbit regulates intracellular trafficking to drive insulin-dependent growth during Drosophila development. Development 145:
Brown, Haley E; Reichert, Marie C; Evans, Timothy A (2018) In Vivo Functional Analysis of Drosophila Robo1 Fibronectin Type-III Repeats. G3 (Bethesda) 8:621-630
Spinner, Michael A; Walla, David A; Herman, Tory G (2018) Drosophila Syd-1 Has RhoGAP Activity That Is Required for Presynaptic Clustering of Bruchpilot/ELKS but Not Neurexin-1. Genetics 208:705-716
Martin, Judy Lisette; Sanders, Erin Nicole; Moreno-Roman, Paola et al. (2018) Long-term live imaging of the Drosophila adult midgut reveals real-time dynamics of division, differentiation and loss. Elife 7:
Okamoto, Naoki; Viswanatha, Raghuvir; Bittar, Riyan et al. (2018) A Membrane Transporter Is Required for Steroid Hormone Uptake in Drosophila. Dev Cell 47:294-305.e7
Li, Hongde; Hurlburt, Alexander J; Tennessen, Jason M (2018) A Drosophila model of combined D-2- and L-2-hydroxyglutaric aciduria reveals a mechanism linking mitochondrial citrate export with oncometabolite accumulation. Dis Model Mech 11:
Caridi, Christopher P; D'Agostino, Carla; Ryu, Taehyun et al. (2018) Nuclear F-actin and myosins drive relocalization of heterochromatic breaks. Nature 559:54-60
Yue, Yang; Blasius, T Lynne; Zhang, Stephanie et al. (2018) Altered chemomechanical coupling causes impaired motility of the kinesin-4 motors KIF27 and KIF7. J Cell Biol 217:1319-1334
Zhou, Li; Lim, Mandy Yu Theng; Kaur, Prameet et al. (2018) Importance of miRNA stability and alternative primary miRNA isoforms in gene regulation during Drosophila development. Elife 7:
Song, Wan; Zsindely, Nóra; Faragó, Anikó et al. (2018) Systematic genetic interaction studies identify histone demethylase Utx as potential target for ameliorating Huntington's disease. Hum Mol Genet 27:649-666

Showing the most recent 10 out of 90 publications