This project would establish a national Xenopus Resource Center at the Marine Biological Laboratory (MBL) in Woods Hole, MA. White Papers submitted to NIH by the Xenopus research community describe the urgent need for a center to house animal stocks and to disseminate new technology. Several laboratories, primarily those who are key personnel on this proposal, have defined the husbandry, health and housing conditions needed to develop a major stock center. The MBL is an ideal place for such a center because it is acknowledged as a premier site for biological research;it has a long association with the Xenopus research community and, as part of a Regenerative Medicine initiative, will fund the construction of a new Xenopus Center if funds are obtained for Center operation in 2009. This resubmission has four aims. The first entails setting up the facility in year 1 and generating critical animal lines (wildtype, inbred, transgenic and mutant).
In aim 2, covering years 2-5, the scope of the facility will be expanded, to provide a spectrum of animal lines recognized as a high priority by the research community. In addition, transgenic and genetic core facilities will be established as part of the new Center to allow new lines to be generated, and for researchers to visit the facility to learn technologies associated with these areas. In the third aim, we focus on the role of the proposed Center in disseminating new technology This would include a series of minicourses to be offered at the Center, to be taught by experts in particular areas (e.g. husbandry, transgenesis, advanced imaging, preparation of egg extracts, bioinformatics).
The final aim focuses on animal husbandry, include setting up protocols for enhanced husbandry and health surveillance, and disseminating these to the research community. Experiments are proposed to optimize husbandry to improve fertility of females and shorten the time to sexual maturity. Some key changes in this resubmission are: 1) an increased emphasis on X. laevis stocks (and concomitant reduction of X. tropicalis stocks) in order to better serve community needs;2) development of a clearer leadership plan;3) reduction in the complexity of aims (e.g. reducing stock number in the Center;removing forward genetics core);and 4) clarification of health and disease outbreak issues.
Cell biologists studying Xenopus have made many breakthroughs in our understanding of cell adhesion and movements, signal transduction and control of the cell cycle, and thus to our understanding of cancer biology, because malignant transformation affects all of these processes in fundamental ways. Developmental biologists studying Xenopus have made transformative insights into processes controlling patterning of and movements in the embryo. and gene regulation, which underlie a multitude of birth defects.
|Harding, Joanne L; Horswell, Stuart; Heliot, Claire et al. (2014) Small RNA profiling of Xenopus embryos reveals novel miRNAs and a new class of small RNAs derived from intronic transposable elements. Genome Res 24:96-106|
|Zheng, Zhenzhen; Christley, Scott; Chiu, William T et al. (2014) Inference of the Xenopus tropicalis embryonic regulatory network and spatial gene expression patterns. BMC Syst Biol 8:3|
|Shen, Wanhua; Liu, Han-Hsuan; Schiapparelli, Lucio et al. (2014) Acute synthesis of CPEB is required for plasticity of visual avoidance behavior in Xenopus. Cell Rep 6:737-47|
|Plouhinec, Jean-Louis; Roche, Daniel D; Pegoraro, Caterina et al. (2014) Pax3 and Zic1 trigger the early neural crest gene regulatory network by the direct activation of multiple key neural crest specifiers. Dev Biol 386:461-72|
|Field, Christine M; Nguyen, Phuong A; Ishihara, Keisuke et al. (2014) Xenopus egg cytoplasm with intact actin. Methods Enzymol 540:399-415|
|Wühr, Martin; Freeman Jr, Robert M; Presler, Marc et al. (2014) Deep proteomics of the Xenopus laevis egg using an mRNA-derived reference database. Curr Biol 24:1467-75|
|Amin, Nirav M; Greco, Todd M; Kuchenbrod, Lauren M et al. (2014) Proteomic profiling of cardiac tissue by isolation of nuclei tagged in specific cell types (INTACT). Development 141:962-73|
|Amin, Nirav M; Gibbs, Devin; Conlon, Frank L (2014) Differential regulation of CASZ1 protein expression during cardiac and skeletal muscle development. Dev Dyn 243:948-56|
|Groen, Aaron C; Ngyuen, Phuong A; Field, Christine M et al. (2014) Glycogen-supplemented mitotic cytosol for analyzing Xenopus egg microtubule organization. Methods Enzymol 540:417-33|
|Chung, Mei-I; Kwon, Taejoon; Tu, Fan et al. (2014) Coordinated genomic control of ciliogenesis and cell movement by RFX2. Elife 3:e01439|
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