Significance: Proteases make up 2% of the human proteome (3), and their functional activity is a unique, irreversible post-translational modification of their target substrates (7). Proteases are involved in a number of important physiological functions from blood coagulation to digestion and pathophysiological activities necessary for activation and propagation of cancer cascades and Alzheimer's disease (8). More information about protease biology and pathophysiology in addition to new selective therapeutics would be important for understanding and treating human disease.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Biotechnology Resource Grants (P41)
Project #
1P41CA196276-01
Application #
8702411
Study Section
Special Emphasis Panel (ZRG1-IMM-N (41))
Project Start
2014-09-24
Project End
2019-08-31
Budget Start
2014-09-24
Budget End
2015-08-31
Support Year
1
Fiscal Year
2014
Total Cost
$342,556
Indirect Cost
$122,556
Name
University of California San Francisco
Department
Type
DUNS #
094878337
City
San Francisco
State
CA
Country
United States
Zip Code
94143
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Roncase, Emily J; Moon, Clara; Chatterjee, Sandip et al. (2017) Substrate Profiling and High Resolution Co-complex Crystal Structure of a Secreted C11 Protease Conserved across Commensal Bacteria. ACS Chem Biol 12:1556-1565
Lin, Shixian; Yang, Xiaoyu; Jia, Shang et al. (2017) Redox-based reagents for chemoselective methionine bioconjugation. Science 355:597-602
Dang, Shangyu; Feng, Shengjie; Tien, Jason et al. (2017) Cryo-EM structures of the TMEM16A calcium-activated chloride channel. Nature 552:426-429
Isaac, R Stefan; Sanulli, Serena; Tibble, Ryan et al. (2017) Biochemical Basis for Distinct Roles of the Heterochromatin Proteins Swi6 and Chp2. J Mol Biol 429:3666-3677
Wilkinson, David J; Habgood, Angela; Lamb, Heather K et al. (2017) Matriptase Induction of Metalloproteinase-Dependent Aggrecanolysis In Vitro and In Vivo: Promotion of Osteoarthritic Cartilage Damage by Multiple Mechanisms. Arthritis Rheumatol 69:1601-1611
O'Donoghue, Anthony J; Ivry, Sam L; Chaudhury, Chaity et al. (2016) Procathepsin E is highly abundant but minimally active in pancreatic ductal adenocarcinoma tumors. Biol Chem 397:871-81
Wong, Kenneth R; Menendez, Elizabeth; Craik, Charles S et al. (2016) In vivo imaging of protease activity by Probody therapeutic activation. Biochimie 122:62-7
Pan, Yuchen; Sackmann, Eric K; Wypisniak, Karolina et al. (2016) Determination of equilibrium dissociation constants for recombinant antibodies by high-throughput affinity electrophoresis. Sci Rep 6:39774
Drake, Christopher R; Sevillano, Natalia; Truillet, Charles et al. (2016) Site-Specific Radiofluorination of Biomolecules with 8-[(18)F]-Fluorooctanoic Acid Catalyzed by Lipoic Acid Ligase. ACS Chem Biol 11:1587-94

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