Significance: Proteases make up 2% of the human proteome (3), and their functional activity is a unique, irreversible post-translational modification of their target substrates (7). Proteases are involved in a number of important physiological functions from blood coagulation to digestion and pathophysiological activities necessary for activation and propagation of cancer cascades and Alzheimer's disease (8). More information about protease biology and pathophysiology in addition to new selective therapeutics would be important for understanding and treating human disease.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Biotechnology Resource Grants (P41)
Project #
1P41CA196276-01
Application #
8702411
Study Section
Special Emphasis Panel (ZRG1-IMM-N (41))
Project Start
2014-09-24
Project End
2019-08-31
Budget Start
2014-09-24
Budget End
2015-08-31
Support Year
1
Fiscal Year
2014
Total Cost
$342,556
Indirect Cost
$122,556
Name
University of California San Francisco
Department
Type
DUNS #
094878337
City
San Francisco
State
CA
Country
United States
Zip Code
94143
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Wong, Kenneth R; Menendez, Elizabeth; Craik, Charles S et al. (2016) In vivo imaging of protease activity by Probody therapeutic activation. Biochimie 122:62-7
Kim, JungMin; Wu, Shenping; Tomasiak, Thomas M et al. (2015) Subnanometre-resolution electron cryomicroscopy structure of a heterodimeric ABC exporter. Nature 517:396-400
LeBeau, Aaron M; Sevillano, Natalia; Markham, Kate et al. (2015) Imaging active urokinase plasminogen activator in prostate cancer. Cancer Res 75:1225-35
Hornsby, Michael; Paduch, Marcin; Miersch, Shane et al. (2015) A High Through-put Platform for Recombinant Antibodies to Folded Proteins. Mol Cell Proteomics 14:2833-47
Li, Keyu; Zettlitz, Kirstin A; Lipianskaya, Julia et al. (2015) A fully human scFv phage display library for rapid antibody fragment reformatting. Protein Eng Des Sel 28:307-16
Le Gall, Marianne; Crépin, Ronan; Neiveyans, Madeline et al. (2015) Neutralization of KIT Oncogenic Signaling in Leukemia with Antibodies Targeting KIT Membrane Proximal Domain 5. Mol Cancer Ther 14:2595-605