The hallmarks of like - motility, adaptation and replication - occur because molecules as Individuals and organized into cells and tissues, generate and respond to forces. The coordinated activity of thousands of molecular motors within single cells oscillates cilia to cause the flow of the pulmonary barrier fluid over long distances. Infection, Inflammation, and metastasis involve the motility of single cells moving through internal changes of shape with forces pushing against their own membranes, peeling, pulling, and rolling with specific proteins in the lumen of blood vessels, or propelled by polymerization of molecular units. Replication involves the wholesale rearrangement of chromosomes through the mitotic spindle, generating forces to organize the chromosomes along the midplate, sensing forces to pass through the checkpoint to finally pull the kinetochores poleward with polymerization forces. Over the past decade the advances in structure identification through genomics and proteomics has been matched by exquisite tools for understanding function through forces. The goal of our resource is to develop force technologies to be applied over a wide range of biological settings, from the single molecule to the tissue level, with integrated systems that orchestrate facile control, multimodal imaging and analysis through visualization and modeling. The individual collaboration projects that steer our technology development cover a wide range of phenomena in molecular biophysics, cell biology and biomedical science. They cover length scales ranging from single molecule (mucin, myosin, MutS) to macromolecular complexes (fibrin fibers, viruses), cells (Plexin, cell motility, cell division), tissue cultures (human lung cell cultures) and macroscopic biomaterial properties (mucus rheology).

Public Health Relevance

Our Resource develops new instrument technologies for enabling flexible force measurements. Including magnetic technologies, integrated atomic force and fluorescence microscopy, and development of software to control magnetic fields and tracking. We continue to bring cutting-edge devices, techniques, and research in computer science to bear on biomedical research, design and implement techniques to display models and real-time simulations aligned in space and time with experimental results.

National Institute of Health (NIH)
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Biotechnology Resource Grants (P41)
Project #
Application #
Study Section
Special Emphasis Panel (ZRG1)
Program Officer
Hunziker, Rosemarie
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of North Carolina Chapel Hill
Schools of Arts and Sciences
Chapel Hill
United States
Zip Code
Woodham, Emma F; Paul, Nikki R; Tyrrell, Benjamin et al. (2017) Coordination by Cdc42 of Actin, Contractility, and Adhesion for Melanoblast Movement in Mouse Skin. Curr Biol 27:624-637
Ye, Feng; Yang, Chansik; Kim, Jiyoon et al. (2017) Epigallocatechin gallate has pleiotropic effects on transmembrane signaling by altering the embedding of transmembrane domains. J Biol Chem 292:9858-9864
Dagliyan, Onur; Karginov, Andrei V; Yagishita, Sho et al. (2017) Engineering Pak1 Allosteric Switches. ACS Synth Biol 6:1257-1262
Takano, Tetsuya; Wu, Mengya; Nakamuta, Shinichi et al. (2017) Discovery of long-range inhibitory signaling to ensure single axon formation. Nat Commun 8:33
Blackmon, R L; Kreda, S M; Sears, P R et al. (2017) Direct monitoring of pulmonary disease treatment biomarkers using plasmonic gold nanorods with diffusion-sensitive OCT. Nanoscale 9:4907-4917
Taylor, A B; Ioannou, M S; Watanabe, T et al. (2017) Perceptually accurate display of two greyscale images as a single colour image. J Microsc 268:73-83
Walton, Bethany L; Lehmann, Marcus; Skorczewski, Tyler et al. (2017) Elevated hematocrit enhances platelet accumulation following vascular injury. Blood 129:2537-2546
Herrington, Kari A; Trinh, Andrew L; Dang, Carolyn et al. (2017) Spatial analysis of Cdc42 activity reveals a role for plasma membrane-associated Cdc42 in centrosome regulation. Mol Biol Cell 28:2135-2145
Speiser, Daniel I; Gagnon, Yakir Luc; Chhetri, Raghav K et al. (2016) Examining the Effects of Chromatic Aberration, Object Distance, and Eye Shape on Image-Formation in the Mirror-Based Eyes of the Bay Scallop Argopecten irradians. Integr Comp Biol 56:796-808
Zdanski, Carlton; Davis, Stephanie; Hong, Yi et al. (2016) Quantitative assessment of the upper airway in infants and children with subglottic stenosis. Laryngoscope 126:1225-31

Showing the most recent 10 out of 151 publications