The proposed research will invent, develop, and deploy novel force microscopes, new molecular markers, and new image analysis to support research in cell biology, thrombosis (clotting), and airways (breathing). Researchers are working with nineteen collaborators working on basic research and health issues ranging from surgery planning for pediatric patients with narrowed airways to cystic fibrosis, blood clotting disorders, and skin and other cancers. The novel microscope technologies include a 12-lens high-throughput robotic microscope, microfabricated post arrays to measure elasticity of blood and mucus, and a microscope that can image both vertically and horizontally through cells. The new molecular markers include molecules that glow to indicate when specific cellular activities occur and molecules that change and influence cell behavior in response to specific frequencies of light. The new image analysis includes merging 3D images from high- resolution and fluorescence microscopes to provide a more complete view inside cells, image analysis techniques that can learn how to distinguish cancerous from normal cells on their own, and methods that tie together both image analysis and genetic information to better predict disease. The results will be brought into broad use by reporting them in publications, by presenting them at conferences, by releasing them as open- source software, by incorporating them into commercial products, and by teaching about them at annual workshops.

Public Health Relevance

The proposed research will invent, develop, and deploy novel force microscopes, new molecular markers, and new image analysis to support research in cell biology, thrombosis (clotting), and airways (breathing). Researchers are working with nineteen collaborators working on basic research and health issues ranging from surgery planning for pediatric patients with narrowed airways to cystic fibrosis, blood clotting disorders, and skin and other cancers. The results will be brought into broad use by reporting them in publications, by presenting them at conferences, by releasing them as open-source software, by incorporating them into commercial products, and by teaching about them at annual workshops. ____________________________________________________________________________

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Biotechnology Resource Grants (P41)
Project #
5P41EB002025-33
Application #
9284455
Study Section
Special Emphasis Panel (ZEB1)
Program Officer
Hunziker, Rosemarie
Project Start
1984-05-01
Project End
2020-04-30
Budget Start
2017-05-01
Budget End
2018-04-30
Support Year
33
Fiscal Year
2017
Total Cost
Indirect Cost
Name
University of North Carolina Chapel Hill
Department
Physics
Type
Schools of Arts and Sciences
DUNS #
608195277
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599
Beicker, Kellie; O'Brien 3rd, E Timothy; Falvo, Michael R et al. (2018) Vertical Light Sheet Enhanced Side-View Imaging for AFM Cell Mechanics Studies. Sci Rep 8:1504
van Haren, Jeffrey; Charafeddine, Rabab A; Ettinger, Andreas et al. (2018) Local control of intracellular microtubule dynamics by EB1 photodissociation. Nat Cell Biol 20:252-261
Stefanini, Lucia; Lee, Robert H; Paul, David S et al. (2018) Functional redundancy between RAP1 isoforms in murine platelet production and function. Blood 132:1951-1962
Yumerefendi, Hayretin; Wang, Hui; Dickinson, Daniel J et al. (2018) Light-Dependent Cytoplasmic Recruitment Enhances the Dynamic Range of a Nuclear Import Photoswitch. Chembiochem 19:1319-1325
Ma, Xiao; Dagliyan, Onur; Hahn, Klaus M et al. (2018) Profiling cellular morphodynamics by spatiotemporal spectrum decomposition. PLoS Comput Biol 14:e1006321
Yan, Connie; Wang, Fei; Peng, Yun et al. (2018) Microtubule Acetylation Is Required for Mechanosensation in Drosophila. Cell Rep 25:1051-1065.e6
Dagliyan, Onur; Krokhotin, Andrey; Ozkan-Dagliyan, Irem et al. (2018) Computational design of chemogenetic and optogenetic split proteins. Nat Commun 9:4042
Boulter, Etienne; Estrach, Soline; Tissot, Floriane S et al. (2018) Cell metabolism regulates integrin mechanosensing via an SLC3A2-dependent sphingolipid biosynthesis pathway. Nat Commun 9:4862
Ye, Feng; Yang, Chansik; Kim, Jiyoon et al. (2017) Epigallocatechin gallate has pleiotropic effects on transmembrane signaling by altering the embedding of transmembrane domains. J Biol Chem 292:9858-9864
Woodham, Emma F; Paul, Nikki R; Tyrrell, Benjamin et al. (2017) Coordination by Cdc42 of Actin, Contractility, and Adhesion for Melanoblast Movement in Mouse Skin. Curr Biol 27:624-637

Showing the most recent 10 out of 163 publications