The proposed research will invent, develop, and deploy novel force microscopes, new molecular markers, and new image analysis to support research in cell biology, thrombosis (clotting), and airways (breathing). Researchers are working with nineteen collaborators working on basic research and health issues ranging from surgery planning for pediatric patients with narrowed airways to cystic fibrosis, blood clotting disorders, and skin and other cancers. The novel microscope technologies include a 12-lens high-throughput robotic microscope, microfabricated post arrays to measure elasticity of blood and mucus, and a microscope that can image both vertically and horizontally through cells. The new molecular markers include molecules that glow to indicate when specific cellular activities occur and molecules that change and influence cell behavior in response to specific frequencies of light. The new image analysis includes merging 3D images from high- resolution and fluorescence microscopes to provide a more complete view inside cells, image analysis techniques that can learn how to distinguish cancerous from normal cells on their own, and methods that tie together both image analysis and genetic information to better predict disease. The results will be brought into broad use by reporting them in publications, by presenting them at conferences, by releasing them as open- source software, by incorporating them into commercial products, and by teaching about them at annual workshops.

Public Health Relevance

The proposed research will invent, develop, and deploy novel force microscopes, new molecular markers, and new image analysis to support research in cell biology, thrombosis (clotting), and airways (breathing). Researchers are working with nineteen collaborators working on basic research and health issues ranging from surgery planning for pediatric patients with narrowed airways to cystic fibrosis, blood clotting disorders, and skin and other cancers. The results will be brought into broad use by reporting them in publications, by presenting them at conferences, by releasing them as open-source software, by incorporating them into commercial products, and by teaching about them at annual workshops. ____________________________________________________________________________

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Biotechnology Resource Grants (P41)
Project #
5P41EB002025-33
Application #
9284455
Study Section
Special Emphasis Panel (ZEB1)
Program Officer
Hunziker, Rosemarie
Project Start
1984-05-01
Project End
2020-04-30
Budget Start
2017-05-01
Budget End
2018-04-30
Support Year
33
Fiscal Year
2017
Total Cost
Indirect Cost
Name
University of North Carolina Chapel Hill
Department
Physics
Type
Schools of Arts and Sciences
DUNS #
608195277
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599
Woodham, Emma F; Paul, Nikki R; Tyrrell, Benjamin et al. (2017) Coordination by Cdc42 of Actin, Contractility, and Adhesion for Melanoblast Movement in Mouse Skin. Curr Biol 27:624-637
Ye, Feng; Yang, Chansik; Kim, Jiyoon et al. (2017) Epigallocatechin gallate has pleiotropic effects on transmembrane signaling by altering the embedding of transmembrane domains. J Biol Chem 292:9858-9864
Dagliyan, Onur; Karginov, Andrei V; Yagishita, Sho et al. (2017) Engineering Pak1 Allosteric Switches. ACS Synth Biol 6:1257-1262
Takano, Tetsuya; Wu, Mengya; Nakamuta, Shinichi et al. (2017) Discovery of long-range inhibitory signaling to ensure single axon formation. Nat Commun 8:33
Blackmon, R L; Kreda, S M; Sears, P R et al. (2017) Direct monitoring of pulmonary disease treatment biomarkers using plasmonic gold nanorods with diffusion-sensitive OCT. Nanoscale 9:4907-4917
Taylor, A B; Ioannou, M S; Watanabe, T et al. (2017) Perceptually accurate display of two greyscale images as a single colour image. J Microsc 268:73-83
Walton, Bethany L; Lehmann, Marcus; Skorczewski, Tyler et al. (2017) Elevated hematocrit enhances platelet accumulation following vascular injury. Blood 129:2537-2546
Herrington, Kari A; Trinh, Andrew L; Dang, Carolyn et al. (2017) Spatial analysis of Cdc42 activity reveals a role for plasma membrane-associated Cdc42 in centrosome regulation. Mol Biol Cell 28:2135-2145
Speiser, Daniel I; Gagnon, Yakir Luc; Chhetri, Raghav K et al. (2016) Examining the Effects of Chromatic Aberration, Object Distance, and Eye Shape on Image-Formation in the Mirror-Based Eyes of the Bay Scallop Argopecten irradians. Integr Comp Biol 56:796-808
Zdanski, Carlton; Davis, Stephanie; Hong, Yi et al. (2016) Quantitative assessment of the upper airway in infants and children with subglottic stenosis. Laryngoscope 126:1225-31

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