The Northeast Collaborative Access Team (NE-CAT) is developing an Undulator Resource for Structural Biology at Sector 24 of the Advanced Photon Source (APS). The main focus of the resource is to optimize high brilliance undulator radiation for technically challenging crystallographic experiments. The NE-CAT sector design utilizes a novel insertion device configuration in which two canted undulators are placed in a single straight section. One undulator provides beam to a tunable end station (24-1D-C), and the second undulator provides beam to a monochromatic side station (24-1D-E). The Sector 24 bending magnet will provide beam to a soon-to-be-completed tunable station (24-BM). Each station is equipped with an ADSC Quantum 315 CCD detector, goniometers, sample cryrocoolers and remote crystal visualization cameras. Crystal automounters will be provided for all three stations. Beamline control is provided by ADSC, CONSOLE and EPICS with either CONSOLE or Blu-lce GUI's. We will develop three core technologies during the next grant period: (1) microdiffraction, (2) hardware for challenging samples and (3) computing for challenging samples. The goal of core 1 is to develop the first beamline in the US dedicated to microdiffraction of biological macromolecules. The goal of core 2 is to optimize beam stability, beam focus and signal-to-noise for challenging samples. The goal of core 3 is to develop flexible beamline control software, and to optimize data collection and processing strategies for non-standard cases. The core technologies will be driven by 11 collaborative science projects involving a wide variety of challenging crystals and a wide range of biological applications. The user program is now operational for beamline 24- ID-C. Beamline 24-ID-E will be operational in the summer of 2007, and 24-BM will be operational in about a year. General users will receive 50% of the available beamtime, and the remaining 50% will go to NE-CAT members. The Resource plans extensive training and dissemination programs including on-site user training, web-based tutorials and screencasts, and off site mini-symposia. We will organize annual workshops related to data collection and structure determination. The Resource will publish a biannual electronic newsletter as a tool to inform the scientific community about NE-CAT developments and as an aid in building our user base. We will continue to maintain an extensive web site, present papers at scientific meetings, publish technical developments and scientific results, and contribute to the APS design library.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Biotechnology Resource Grants (P41)
Project #
8P41GM103403-10
Application #
8242009
Study Section
Special Emphasis Panel (ZRG1-BCMB-K (40))
Program Officer
Wu, Mary Ann
Project Start
2001-06-15
Project End
2013-03-31
Budget Start
2012-04-01
Budget End
2013-03-31
Support Year
10
Fiscal Year
2012
Total Cost
$2,042,492
Indirect Cost
$447,841
Name
Cornell University
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
872612445
City
Ithaca
State
NY
Country
United States
Zip Code
14850
Lee, Won-Gil; Chan, Albert H; Spasov, Krasimir A et al. (2016) Design, Conformation, and Crystallography of 2-Naphthyl Phenyl Ethers as Potent Anti-HIV Agents. ACS Med Chem Lett 7:1156-1160
Morales-Perez, Claudio L; Noviello, Colleen M; Hibbs, Ryan E (2016) X-ray structure of the human α4β2 nicotinic receptor. Nature 538:411-415
Silvaroli, Josie A; Arne, Jason M; Chelstowska, Sylwia et al. (2016) Ligand Binding Induces Conformational Changes in Human Cellular Retinol-binding Protein 1 (CRBP1) Revealed by Atomic Resolution Crystal Structures. J Biol Chem 291:8528-40
Baytshtok, Vladimir; Fei, Xue; Grant, Robert A et al. (2016) A Structurally Dynamic Region of the HslU Intermediate Domain Controls Protein Degradation and ATP Hydrolysis. Structure 24:1766-1777
Chowdhury, Chiranjit; Chun, Sunny; Sawaya, Michael R et al. (2016) The function of the PduJ microcompartment shell protein is determined by the genomic position of its encoding gene. Mol Microbiol 101:770-83
Atkison, James H; Parnham, Stuart; Marcotte Jr, William R et al. (2016) Crystal Structure of the Nephila clavipes Major Ampullate Spidroin 1A N-terminal Domain Reveals Plasticity at the Dimer Interface. J Biol Chem 291:19006-17
Gorelik, Maryna; Orlicky, Stephen; Sartori, Maria A et al. (2016) Inhibition of SCF ubiquitin ligases by engineered ubiquitin variants that target the Cul1 binding site on the Skp1-F-box interface. Proc Natl Acad Sci U S A 113:3527-32
Bale, Jacob B; Gonen, Shane; Liu, Yuxi et al. (2016) Accurate design of megadalton-scale two-component icosahedral protein complexes. Science 353:389-94
Shan, Chun-Min; Wang, Jiyong; Xu, Ke et al. (2016) A histone H3K9M mutation traps histone methyltransferase Clr4 to prevent heterochromatin spreading. Elife 5:
Clark, Nathaniel E; Katolik, Adam; Roberts, Kenneth M et al. (2016) Metal dependence and branched RNA cocrystal structures of the RNA lariat debranching enzyme Dbr1. Proc Natl Acad Sci U S A 113:14727-14732

Showing the most recent 10 out of 321 publications