The Washington University NIGMS Biomedical Resource proposes to continue its 37 years of support for biomedical research through the development of technology based on mass spectrometry (MS). The Resource will pursue this in three areas: 1) The characterization of complex lipids and lipid biosynthesis with a focus on a) pathogenic microorganisms like trypanosomes and mycobacteria and the biosynthetic pathways that offer targets for therapy, b) the characterization of lipid antigens, and c) lipid mediators o fatty acid synthase; 2) The establishment of a biophysics center that offers MS-based tools to probe protein dynamics, protein interactions, protein complex stoichiometry and structure, and membrane proteins using a) hydrogen deuterium exchange MS, b) fast photochemical oxidation of proteins, c) top-down analysis of proteins, and d) crosslinking methods for complexes; and 3) the extension of high energy collisional activation methods for the study of complex lipids and large peptides, especially those predicted from genomic data. This technology development will be driven by nearly three dozen collaborative efforts that are broad in scientific and geographic scope and focused on the study of human health and disease. The technology will be made available to other collaborators through our extensive dissemination and training efforts.

Public Health Relevance

The Washington University Biomedical Mass Spectrometry Resource has a longstanding history as an active and productive citizen in the NIH Biomedical Technology Research Resources community. We propose to extend our mission by advancing mass spectrometry technology, development, and research, applying these discoveries to answer critical biomedical research questions, and training the next generation of researchers, towards the ultimate goal of improving public health.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Biotechnology Resource Grants (P41)
Project #
5P41GM103422-41
Application #
9412473
Study Section
Special Emphasis Panel (ZRG1)
Program Officer
Wu, Mary Ann
Project Start
1997-08-01
Project End
2019-12-31
Budget Start
2018-01-01
Budget End
2018-12-31
Support Year
41
Fiscal Year
2018
Total Cost
Indirect Cost
Name
Washington University
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
068552207
City
Saint Louis
State
MO
Country
United States
Zip Code
63130
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