This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Forces resulting from acoustic radiation pressure are an effective means to localize particles in an arrangement similar to hydrodynamic focusing without the need for sheath fluids. A recently developed acoustic device that has been proven effective in sheath replacement is the line-driven capillary [G. Goddard, et al., Cytometry 69A, 66-74 (2006)]. It is constructed from a capillary that is driven by a piezoceramic source in line-contact with its outer wall. Vibration of the structure creates a localized pressure node along the central axis where an axial particle trap is formed. The implementation of acoustic particle focusing in flow cytometers will enable new flow cytometry techniques and methods to evolve due to fundamental changes in the way particles are positioned within the sample cell. The advantages of this new type of sample delivery include: i.) longer particle interrogation times without concurrent loss in particle analysis rate, ii.) ability to freely stop and reverse the flow direction without loss of particle alignment for particle reanalysis, iii.) induced particle orientation in the optical scattering plane, and iv.) in-line concentration and size fractionation of dilute samples. This proposal is a tailored implementation of acoustic line-driven capillaries to develop new measurements and in-line sampling methods that are made possible by replacing hydrodynamic sheath flow with acoustically focused particle streams. First, we will develop enhanced detection capabilities that utilize slow-flow, stop-flow, and reverse-flow conditions in an acoustically focused flow chamber. Our applications will include investigation of statistical data enhancements that can be achieved when particle reanalysis is allowed and the flow stream can be reversed to reanalyze particles of high significance. Second, acoustic slow-flow (long transit time) particle delivery will be studied for a system based upon time-resolved luminescence detection using lanthanide chelate probes to provide a means for autofluorescence rejection and increased spectral multiplexing. Third, we will investigate acoustic control of particle orientation in the optical analysis plane to study enhanced scattering signatures of asymmetric particles that are oriented in a repeatable fashion in the optical interrogation region. Finally, we will develop acoustic field-based particle size selection for pre-analysis in-line sample purification and pre-sorting for flow cytometers

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001315-30
Application #
8361768
Study Section
Special Emphasis Panel (ZRG1-CB-K (40))
Project Start
2011-04-01
Project End
2013-03-31
Budget Start
2011-04-01
Budget End
2013-03-31
Support Year
30
Fiscal Year
2011
Total Cost
$89,451
Indirect Cost
Name
Los Alamos National Lab
Department
Type
DUNS #
175252894
City
Los Alamos
State
NM
Country
United States
Zip Code
87545
Johnson, Leah M; Gao, Lu; Shields IV, C Wyatt et al. (2013) Elastomeric microparticles for acoustic mediated bioseparations. J Nanobiotechnology 11:22
Ai, Ye; Sanders, Claire K; Marrone, Babetta L (2013) Separation of Escherichia coli bacteria from peripheral blood mononuclear cells using standing surface acoustic waves. Anal Chem 85:9126-34
Micheva-Viteva, Sofiya N; Shou, Yulin; Nowak-Lovato, Kristy L et al. (2013) c-KIT signaling is targeted by pathogenic Yersinia to suppress the host immune response. BMC Microbiol 13:249
Sanders, Claire K; Mourant, Judith R (2013) Advantages of full spectrum flow cytometry. J Biomed Opt 18:037004
Cushing, Kevin W; Piyasena, Menake E; Carroll, Nick J et al. (2013) Elastomeric negative acoustic contrast particles for affinity capture assays. Anal Chem 85:2208-15
Piyasena, Menake E; Austin Suthanthiraraj, Pearlson P; Applegate Jr, Robert W et al. (2012) Multinode acoustic focusing for parallel flow cytometry. Anal Chem 84:1831-9
Austin Suthanthiraraj, Pearlson P; Piyasena, Menake E; Woods, Travis A et al. (2012) One-dimensional acoustic standing waves in rectangular channels for flow cytometry. Methods 57:259-71
Vuyisich, Momchilo; Sanders, Claire K; Graves, Steven W (2012) Binding and cell intoxication studies of anthrax lethal toxin. Mol Biol Rep 39:5897-903
Chaudhary, Anu; Ganguly, Kumkum; Cabantous, Stephanie et al. (2012) The Brucella TIR-like protein TcpB interacts with the death domain of MyD88. Biochem Biophys Res Commun 417:299-304
Marina, Oana C; Sanders, Claire K; Mourant, Judith R (2012) Effects of acetic acid on light scattering from cells. J Biomed Opt 17:085002-1

Showing the most recent 10 out of 239 publications