This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The investigation of venoms is focused on the knowledge of the components directly related to the toxic effects for humans, but many components directly related to the toxic effects for humans, but many components have a broad spectrum of biological activities. This, in addition to the medical reasons, the study of the composition of the venom has a great interest for the discovery of new biochemical tools for the analysis of the operational mechanisms of the nervous system and cellular membranes. It has been reported that scorpion venom contains a variety of biologically active components: enzymes, peptides, nucleotides, lipids, biogenic amines and other unknown substances. Scorpion venoms are rich sources of peptides with a variety of pharmacological functions. Known pharmacological activities include antimicrobial, anti-epilepsy, insecticide, phospholipase and toxins. The scorpion Vaejovis mexicanus smithi is native from the state of Morelos, Mexico. This species has not been studied in depth previously. Preliminary studies have shown a complex mixture of components present in its venom, which makes it an interesting target of study for the search of new bioactive components. Crude venom was fractionated by RP-HPLC, and around 85 fractions were collected. Each fraction was subjected to MALDI-TOF mass spectrometry. The approximate size of different cannel-blocking peptides has been previously reported. Fractions containing components of such molecular masses were selected for further studies. Due to the complexity of the mixture and the size of the peptides of interest, Edman sequencing as well as LC/MS, CID and ECD could be utilized in order to decipher the amino acid sequence of the toxin components. A combination ECD analysis and selective N-terminal labeling can aid the de novo sequencing of large peptides.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001614-29
Application #
8363774
Study Section
Special Emphasis Panel (ZRG1-BCMB-M (40))
Project Start
2011-06-01
Project End
2012-05-31
Budget Start
2011-06-01
Budget End
2012-05-31
Support Year
29
Fiscal Year
2011
Total Cost
$50
Indirect Cost
Name
University of California San Francisco
Department
Pharmacology
Type
Schools of Pharmacy
DUNS #
094878337
City
San Francisco
State
CA
Country
United States
Zip Code
94143
Liu, Tzu-Yu; Huang, Hector H; Wheeler, Diamond et al. (2017) Time-Resolved Proteomics Extends Ribosome Profiling-Based Measurements of Protein Synthesis Dynamics. Cell Syst 4:636-644.e9
Twiss, Jeffery L; Fainzilber, Mike (2016) Neuroproteomics: How Many Angels can be Identified in an Extract from the Head of a Pin? Mol Cell Proteomics 15:341-3
Bikle, Daniel D (2016) Extraskeletal actions of vitamin D. Ann N Y Acad Sci 1376:29-52
Julien, Olivier; Zhuang, Min; Wiita, Arun P et al. (2016) Quantitative MS-based enzymology of caspases reveals distinct protein substrate specificities, hierarchies, and cellular roles. Proc Natl Acad Sci U S A 113:E2001-10
Bongrand, Clotilde; Koch, Eric J; Moriano-Gutierrez, Silvia et al. (2016) A genomic comparison of 13 symbiotic Vibrio fischeri isolates from the perspective of their host source and colonization behavior. ISME J 10:2907-2917
Cil, Onur; Phuan, Puay-Wah; Lee, Sujin et al. (2016) CFTR activator increases intestinal fluid secretion and normalizes stool output in a mouse model of constipation. Cell Mol Gastroenterol Hepatol 2:317-327
Kintzer, Alexander F; Stroud, Robert M (2016) Structure, inhibition and regulation of two-pore channel TPC1 from Arabidopsis thaliana. Nature 531:258-62
Bradshaw, J Michael; McFarland, Jesse M; Paavilainen, Ville O et al. (2015) Prolonged and tunable residence time using reversible covalent kinase inhibitors. Nat Chem Biol 11:525-31
Bikle, Daniel D (2014) Vitamin D metabolism, mechanism of action, and clinical applications. Chem Biol 21:319-29
Correia, Maria Almira; Wang, YongQiang; Kim, Sung-Mi et al. (2014) Hepatic cytochrome P450 ubiquitination: conformational phosphodegrons for E2/E3 recognition? IUBMB Life 66:78-88

Showing the most recent 10 out of 625 publications