This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.Special precautions should be taken to keep the temperature constant throughout relaxation experiments. The effect of sample heating may produce a significant bias in the extracted relaxation data and subsequently in parameters of molecular motions. First, the extracted relaxation data are affected by the temperature variations between data points of the individual relaxation experiments (e.g. R2 data points recorded with different relaxation delay). Second, a different mean temperature in different types of relaxation experiments results in inconsistency between experimental relaxation data. Several experimental approaches are successful used on RT probes to keep the temperature constant for different data points and between different experiments. & nbsp; However in case of cold probe the sample heating might be somehow larger comparing with RT probe. The goals of our experiments are to compare & nbsp; experimental data recorded on RT and cold probes, and attempt to correct temperature instability on a cold probe.
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