This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Our current Ku-band ESR microimaging system includes a large (15 inch pole face) resistive magnet that provides good working space for ESR microimaging (ESRM) development purposes but is powered by an older Varian V-2500 linear regulated power source to which we have attached a patchwork of several discrete feedback systems for the purpose of accurate field regulation. Currently the feedback systems include a Hall-input Field Dial console, a Lakeshore model 475 Hall field controller and a modified Varian V-4542 field/frequency lock (FFL) system consisting of EPR probe and signal processing electronics. Although this system has performed in a generally satisfactory manner to date, its reliability is marginal, particularly when operating in (Hall-stabilized) field regulation mode for setup purposes, and its use, from an operational viewpoint, is unnecessarily complicated. During the course of upgrading to the new 15KW Gensys magnet power supply (Subproject 0218), we propose replacing the existing regulation system with a newly-designed hybrid system incorporating the present Lakeshore 475 Hall field controller and Varian V-4542 field/frequency lock probe, adding the necessary mode control electronics and communication channel to the Gensys power source. This highly integrated regulation system will also include the programmable precision reference required for offsetting the relatively low resolution (16bit) Lakeshore controller output to achieve ca 20b/10 mG resolution to 1.0T. An option for input dithering can also be included as may be required. Provision is included in current design specification for full remote control of programmable field regulation parameters and functional modes so that the system can operate compatibly with the ESRM LabView VI (virtual instrument) software. We plan to employ essentially the same design in the construction of a second hybrid regulation system to provide Hall/FFL magnetic field control for our proposed 35 GHz ESR microscope (Subproject 0138).

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
2P41RR016292-11
Application #
8364056
Study Section
Special Emphasis Panel (ZRG1-BCMB-K (40))
Project Start
2011-09-01
Project End
2012-08-31
Budget Start
2011-09-01
Budget End
2012-08-31
Support Year
11
Fiscal Year
2011
Total Cost
$4,623
Indirect Cost
Name
Cornell University
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
872612445
City
Ithaca
State
NY
Country
United States
Zip Code
14850
Jain, Rinku; Vanamee, Eva S; Dzikovski, Boris G et al. (2014) An iron-sulfur cluster in the polymerase domain of yeast DNA polymerase ?. J Mol Biol 426:301-8
Pratt, Ashley J; Shin, David S; Merz, Gregory E et al. (2014) Aggregation propensities of superoxide dismutase G93 hotspot mutants mirror ALS clinical phenotypes. Proc Natl Acad Sci U S A 111:E4568-76
Georgieva, Elka R; Borbat, Peter P; Ginter, Christopher et al. (2013) Conformational ensemble of the sodium-coupled aspartate transporter. Nat Struct Mol Biol 20:215-21
Airola, Michael V; Sukomon, Nattakan; Samanta, Dipanjan et al. (2013) HAMP domain conformers that propagate opposite signals in bacterial chemoreceptors. PLoS Biol 11:e1001479
Airola, Michael V; Huh, Doowon; Sukomon, Nattakan et al. (2013) Architecture of the soluble receptor Aer2 indicates an in-line mechanism for PAS and HAMP domain signaling. J Mol Biol 425:886-901
Sun, Yan; Zhang, Ziwei; Grigoryants, Vladimir M et al. (2012) The internal dynamics of mini c TAR DNA probed by electron paramagnetic resonance of nitroxide spin-labels at the lower stem, the loop, and the bulge. Biochemistry 51:8530-41
Smith, Andrew K; Freed, Jack H (2012) Dynamics and ordering of lipid spin-labels along the coexistence curve of two membrane phases: an ESR study. Chem Phys Lipids 165:348-61
Yu, Renyuan Pony; Darmon, Jonathan M; Hoyt, Jordan M et al. (2012) High-Activity Iron Catalysts for the Hydrogenation of Hindered, Unfunctionalized Alkenes. ACS Catal 2:1760-1764
Gaffney, Betty J; Bradshaw, Miles D; Frausto, Stephen D et al. (2012) Locating a lipid at the portal to the lipoxygenase active site. Biophys J 103:2134-44
Dzikovski, Boris; Tipikin, Dmitriy; Freed, Jack (2012) Conformational distributions and hydrogen bonding in gel and frozen lipid bilayers: a high frequency spin-label ESR study. J Phys Chem B 116:6694-706

Showing the most recent 10 out of 72 publications