Remediation of Superfund sites can release nanoscale particles into the environment, along with hazardous vapors. The health effects of these complex mixtures and materials, especially emerging materials produced by the nanotechnology industry, are not sufficiently well understood. It has been hypothesized that the adverse health effects due to exposure to environmental particles (e.g., airborne particulate matter) is, at least in part, due to generation of reactive oxygen species (ROS), but more data are needed to test this mechanism. This project focuses on 3 main areas: (I) using a laboratory combustion system to create complex mixtures of toxic by-products emitted from thermal processing of materials that contain brominated flame retardants;(II) characterizing the generation of reactive oxygen species from nanoscale materials in the environment;and (III) making use of nanotechnology to answer questions that have arisen in earlier studies of nanoparticle toxicity. In the first aim, this project will construct a small, laboratory-scale system to simulate the thermal processing of materials containing brominated flame retardants;the gaseous and particulate by-products of this process will be examined by other projects in this proposal using bioassays for polybrominated diphenyl ethers (PBDEs) and brominated dioxins and furans. In the second aim we will quantitatively measure two of the most important ROS - hydroxyl radical (OH) and hydrogen peroxide (H{2}O{2}) - formed by nanoparticles in a cell-free surrogate lung fluid and, later, in cell cultures. We will also examine how simulated atmospheric reactions alter the ability of nanoparticles to form ROS. In the third aim of this project we will synthesize a range of novel, multi-functional nanoparticles in order to study several questions related to nanomaterial toxicity, in part in conjunction with other projects in the UC Davis Superfund Program.

Public Health Relevance

We will develop and evaluate methods and technologies to detect hazardous substances in the environment, and develop advanced techniques to assess and evaluate the effects of hazardous substances on human health. In addition, our planned work should help in the assessment of the possible effects of nanomaterials on human health, including nanoparticles designed by the UC Davis Superfund Project to detect hazardous substances in the environment.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Hazardous Substances Basic Research Grants Program (NIEHS) (P42)
Project #
5P42ES004699-27
Application #
8450324
Study Section
Special Emphasis Panel (ZES1-LWJ-M)
Project Start
Project End
Budget Start
2013-04-01
Budget End
2014-03-31
Support Year
27
Fiscal Year
2013
Total Cost
$146,169
Indirect Cost
$52,360
Name
University of California Davis
Department
Type
DUNS #
047120084
City
Davis
State
CA
Country
United States
Zip Code
95618
Mao, Yuxin; Pan, Yang; Li, Xuan et al. (2018) High-precision digital droplet pipetting enabled by a plug-and-play microfluidic pipetting chip. Lab Chip 18:2720-2729
Burmistrov, Vladimir; Morisseau, Christophe; Harris, Todd R et al. (2018) Effects of adamantane alterations on soluble epoxide hydrolase inhibition potency, physical properties and metabolic stability. Bioorg Chem 76:510-527
Stamou, Marianna; Grodzki, Ana Cristina; van Oostrum, Marc et al. (2018) Fc gamma receptors are expressed in the developing rat brain and activate downstream signaling molecules upon cross-linking with immune complex. J Neuroinflammation 15:7
Huo, Jingqian; Li, Zhenfeng; Wan, Debin et al. (2018) Development of a Highly Sensitive Direct Competitive Fluorescence Enzyme Immunoassay Based on a Nanobody-Alkaline Phosphatase Fusion Protein for Detection of 3-Phenoxybenzoic Acid in Urine. J Agric Food Chem 66:11284-11290
Zamuruyev, Konstantin O; Borras, Eva; Pettit, Dayna R et al. (2018) Effect of temperature control on the metabolite content in exhaled breath condensate. Anal Chim Acta 1006:49-60
Zamuruyev, Konstantin O; Schmidt, Alexander J; Borras, Eva et al. (2018) Power-efficient self-cleaning hydrophilic condenser surface for portable exhaled breath condensate (EBC) metabolomic sampling. J Breath Res 12:036020
Philippat, Claire; Barkoski, Jacqueline; Tancredi, Daniel J et al. (2018) Prenatal exposure to organophosphate pesticides and risk of autism spectrum disorders and other non-typical development at 3 years in a high-risk cohort. Int J Hyg Environ Health 221:548-555
Burmistrov, Vladimir; Morisseau, Christophe; Pitushkin, Dmitry et al. (2018) Adamantyl thioureas as soluble epoxide hydrolase inhibitors. Bioorg Med Chem Lett 28:2302-2313
Wang, Weicang; Yang, Jun; Zhang, Jianan et al. (2018) Lipidomic profiling reveals soluble epoxide hydrolase as a therapeutic target of obesity-induced colonic inflammation. Proc Natl Acad Sci U S A 115:5283-5288
Tu, Ranran; Armstrong, Jillian; Lee, Kin Sing Stephen et al. (2018) Soluble epoxide hydrolase inhibition decreases reperfusion injury after focal cerebral ischemia. Sci Rep 8:5279

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