To understand the effect of environmental exposures on people and the resulting potential health effects, rapid, accurate methods to measure exposures are needed. An immunoassay is a simple and sensitive measurement method that uses antibodies as detectors. This project makes, improves and uses immunoassays for chemicals that have potential for widespread exposure and a potential impact on human health with the goal preventing or minimizing exposures. Using concepts pioneered in this group, mimics of the compound are chemically or biologically synthesized. The mimics are used to raise antibodies that are specific for the environmental compound. The mimics and antibodies are formatted into assays that are analytically well characterized. The assays are then applied to various studies of human or environmental exposure. Among environmental contaminants to be addressed by this project are the insecticides, permethrin, fipronil and chlorantraniliprole. In addition to toxicity to non target organisms such as fish and aquatic invertebrates, these insecticides are used in agricultural settings, at home, as well as by pest control operators. Exposure to the general population is likely. Assays are also proposed for the brominated flame retardants, polybrominated diphenyl ethers (PBDE) and tetrabromobisphenol A and, newly emerging environmental contaminants, the antimicrobial compounds triclosan and triclocarban. PBDEs appear in the environment through waste disposal of electronics and furniture while antimicrobial compounds are resistant to sewage treatment. To assess human exposure, assays to the human metabolites of these compounds are also proposed. Novel magneticluminescent labels for simultaneous measurement of multiple compounds will be developed to speed throughput. A technique called phage display will be use to make mimics of key assay reagents in order to make assays more selective.

Public Health Relevance

As a result of this project more rapid, sensitive and ultimately more portable analytical methods will be available. Such tools allow researchers to gather more comprehensive information on the relationship of human exposure to environmental contaminants.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Hazardous Substances Basic Research Grants Program (NIEHS) (P42)
Project #
5P42ES004699-28
Application #
8659361
Study Section
Special Emphasis Panel (ZES1-LWJ-M)
Project Start
Project End
Budget Start
2014-04-01
Budget End
2015-03-31
Support Year
28
Fiscal Year
2014
Total Cost
$227,170
Indirect Cost
$79,177
Name
University of California Davis
Department
Type
DUNS #
047120084
City
Davis
State
CA
Country
United States
Zip Code
95618
Fritsch, Erika B; Stegeman, John J; Goldstone, Jared V et al. (2015) Expression and function of ryanodine receptor related pathways in PCB tolerant Atlantic killifish (Fundulus heteroclitus) from New Bedford Harbor, MA, USA. Aquat Toxicol 159:156-66
Flannery, Brenna M; Silverman, Jill L; Bruun, Donald A et al. (2015) Behavioral assessment of NIH Swiss mice acutely intoxicated with tetramethylenedisulfotetramine. Neurotoxicol Teratol 47:36-45
Anderson, Donald S; Silva, Rona M; Lee, Danielle et al. (2015) Persistence of silver nanoparticles in the rat lung: Influence of dose, size, and chemical composition. Nanotoxicology 9:591-602
Yang, Jun; Bratt, Jennifer; Franzi, Lisa et al. (2015) Soluble epoxide hydrolase inhibitor attenuates inflammation and airway hyperresponsiveness in mice. Am J Respir Cell Mol Biol 52:46-55
Davidson, R Andrew; Anderson, Donald S; Van Winkle, Laura S et al. (2015) Evolution of silver nanoparticles in the rat lung investigated by X-ray absorption spectroscopy. J Phys Chem A 119:281-9
Van Winkle, Laura S; Bein, Keith; Anderson, Donald et al. (2015) Biological dose response to PM2.5: effect of particle extraction method on platelet and lung responses. Toxicol Sci 143:349-59
Radhakrishnan, Rajeswaran; Suni, Ian I; Bever, Candace S et al. (2014) Impedance Biosensors: Applications to Sustainability and Remaining Technical Challenges. ACS Sustain Chem Eng 2:1649-1655
Das, Gautom Kumar; Bonifacio, Cecile S; De Rojas, Julius et al. (2014) Ultra-long Magnetic Nanochains for Highly Efficient Arsenic Removal from Water. J Mater Chem A Mater Energy Sustain 2:12974-12981
DeGroot, Danica E; Hayashi, Ai; Denison, Michael S (2014) Lack of ligand-selective binding of the aryl hydrocarbon receptor to putative DNA binding sites regulating expression of Bax and paraoxonase 1 genes. Arch Biochem Biophys 541:13-20
Ogunyoku, Temitope A; Young, Thomas M (2014) Removal of triclocarban and triclosan during municipal biosolid production. Water Environ Res 86:197-203

Showing the most recent 10 out of 872 publications