The Chemistry and Analytical Core of the UNC-SRP is a research support core that provides analytical support to the research projects and synthesis, purification and characterization of chemicals that are not available commercially or that can be cost-effectively prepared in-house. The Chemistry Core offers particular expertise in preparation of standards and internal standards labeled with stable isotopes ([15]N, [13]C, [2]H) that allow development of assays using mass spectrometric techniques for highly specific and highly sensitive identification and quantitation. The specific goals of the Chemistry Core are based on the aims and accomplishments of the individual research projects, and thus will evolve in response to research needs. The overall aims of the Chemistry and Analytical Core include: (1) provide advice and consultation on analytical methodology and chemical handling, including analytical method development, appropriate choice of analytes, instrumentation, and standards (Projects 1, 2, 3, 4 and 5);(2) develop analytical methodology, including gas and liquid chromatography, for detection and quantitation of parent compounds, degradation products, metabolites, and macromolecular adducts (Projects 1, 2, and 5);(3) provide service in carrying out routine assays for detection and measurement by quantitative spectroscopy and mass spectrometry of PAH, PAH metabolites, PCBs, oxidative damage, and macromolecular adducts (Projects 1, 2, 3 and 5);(4) prepare novel and rare chemicals, including isotope-labeled chemicals, devise synthetic routes and offer advice and guidance for UNC-SRP researchers wishing to undertake syntheses and product purification with their own personnel, or carry out the entire preparation as needed (Projects 1, 2, 4 and 5);(5) analysis and structural identification of unknown degradation products, metabolites, and macromolecular adducts and provide expertise in interpretation of spectral data in support of structural elucidation (Projects 1, 2, 4 and 5). Specific major tasks include preparation of [[13]C]-labeled vinyl carbamate and its epoxide for Project 1, assisting Project 2 with assay of trichloroethylene metabolites, assisting Project 4 with assays of environmental complex mixtures, assisting Project 5 with fractionation of extracts of bioremediated soil and identification of genotoxically-active constituents of the extracts and preparation of [U-[13]C] PAH quinones, and providing assays of biomarkers of oxidative stress as needed for Projects 1-5.

Public Health Relevance

The syntheses and analytical services offered by the Chemistry and Analytical Core will provide the infrastructure for analyses and assays that are critical for evaluating exposure to hazardous environmental chemicals and for understanding the role of oxidative stress in determining health outcomes, in the context of improving the science supporting assessment of the risks associated with exposure to chemicals at Superfund hazardous waste sites.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Hazardous Substances Basic Research Grants Program (NIEHS) (P42)
Project #
3P42ES005948-21S1
Application #
8885023
Study Section
Special Emphasis Panel (ZES1)
Project Start
Project End
Budget Start
2014-04-01
Budget End
2015-03-31
Support Year
21
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of North Carolina Chapel Hill
Department
Type
DUNS #
City
Chapel Hill
State
NC
Country
United States
Zip Code
Reif, David M; Truong, Lisa; Mandrell, David et al. (2016) High-throughput characterization of chemical-associated embryonic behavioral changes predicts teratogenic outcomes. Arch Toxicol 90:1459-70
Brooks, Samira A; Martin, Elizabeth; Smeester, Lisa et al. (2016) miRNAs as common regulators of the transforming growth factor (TGF)-β pathway in the preeclamptic placenta and cadmium-treated trophoblasts: Links between the environment, the epigenome and preeclampsia. Food Chem Toxicol 98:50-57
Wu, Tao P; Wang, Tao; Seetin, Matthew G et al. (2016) DNA methylation on N(6)-adenine in mammalian embryonic stem cells. Nature 532:329-33
Zabinski, Joseph W; Garcia-Vargas, Gonzalo; Rubio-Andrade, Marisela et al. (2016) Advancing Dose-Response Assessment Methods for Environmental Regulatory Impact Analysis: A Bayesian Belief Network Approach Applied to Inorganic Arsenic. Environ Sci Technol Lett 3:200-204
Tian, Xu; Patel, Keyur; Ridpath, John R et al. (2016) Homologous Recombination and Translesion DNA Synthesis Play Critical Roles on Tolerating DNA Damage Caused by Trace Levels of Hexavalent Chromium. PLoS One 11:e0167503
Smith, Martyn T; Guyton, Kathryn Z; Gibbons, Catherine F et al. (2016) Key Characteristics of Carcinogens as a Basis for Organizing Data on Mechanisms of Carcinogenesis. Environ Health Perspect 124:713-21
Chappell, Grace; Silva, Grace O; Uehara, Takeki et al. (2016) Characterization of copy number alterations in a mouse model of fibrosis-associated hepatocellular carcinoma reveals concordance with human disease. Cancer Med 5:574-85
Sharma, Vyom; Collins, Leonard B; Chen, Ting-Huei et al. (2016) Oxidative stress at low levels can induce clustered DNA lesions leading to NHEJ mediated mutations. Oncotarget 7:25377-90
Lai, Yongquan; Yu, Rui; Hartwell, Hadley J et al. (2016) Measurement of Endogenous versus Exogenous Formaldehyde-Induced DNA-Protein Crosslinks in Animal Tissues by Stable Isotope Labeling and Ultrasensitive Mass Spectrometry. Cancer Res 76:2652-61
Adrion, Alden C; Nakamura, Jun; Shea, Damian et al. (2016) Screening Nonionic Surfactants for Enhanced Biodegradation of Polycyclic Aromatic Hydrocarbons Remaining in Soil After Conventional Biological Treatment. Environ Sci Technol 50:3838-45

Showing the most recent 10 out of 453 publications