The Analytical Core provides routine and non-routine analytical service to the University of lowa research program. The core provides expert staff, equipment, and instrumentation in line with five Aims.
Specific Aim 1 is the development of analytical and quality control/assurance standards. In close collaboration with the Synthesis Core, which is responsible for acquiring and archiving individual compound standards and preparing stock solutions, the Analytical Core develops the mathematical methods for congener representation, analyzes single congener solutions for purity assessment and quality control, and tests the prepared standard solutions against certified Standard Reference Materials, including PCB calibration mixtures.
Specific Aim 2 is the development of a mixture of PCB congeners for laboratory animal exposure studies. The Analytical Core develop the mathematical methods to design the exposure mixture and tests the prepared mixtures against certified Standard Reference Materials.
Specific Aim 3 is the analysis of environmental and laboratory samples for a suite of PCB congener. The Analytical Core will extract and analyze PCBs on a congener-specific basis: 209 congeners are detected as individual or coeluting sets. We typically achieve 170 congener separations in environmental samples. Applying the primary calibration standard developed under Aim 1, samples are quantified by the internal standard method. Internal Standards and Surrogate Recovery Standards developed under Aim 1 are used to precisely determine PCB concentrations in samples. Methods for analysis on a congener-specific basis are assessed using performance standard injections and analysis of certified Standard Reference Materials. Most analyses are performed on the entire suite of PCB congeners, including laboratory animals exposed to the mixtures described in Aim 2 and including blood from humans and non-laboratory animals. Other samples are analyzed for specific congeners and metabolites in support of investigators focused on congener-specific toxicology.
Specific Aim 4 involves the extraction, identification, and quantification of unknown metabolites. The Analytical Core determines purity of synthesized metabolites and associated analytical standards, then analyzes for hydroxylated PCBs and other PCB metabolites. As requested by the isbrp investigators, the Analytical Core will also train personnel and assist in sampling design and implementation.
Specific Aim 5 is the design and maintenance of a database system. A redundant storage architecture with automatic backup and password protection is used to allow investigators to download data and reports produced by the Analytical Core.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Hazardous Substances Basic Research Grants Program (NIEHS) (P42)
Project #
5P42ES013661-07
Application #
8377295
Study Section
Special Emphasis Panel (ZES1-LWJ-M)
Project Start
Project End
Budget Start
2012-04-01
Budget End
2013-03-31
Support Year
7
Fiscal Year
2012
Total Cost
$338,014
Indirect Cost
$108,106
Name
University of Iowa
Department
Type
DUNS #
062761671
City
Iowa City
State
IA
Country
United States
Zip Code
52242
Wu, Xianai; Yang, Jun; Morisseau, Christophe et al. (2016) 3,3',4,4',5-Pentachlorobiphenyl (PCB 126) Decreases Hepatic and Systemic Ratios of Epoxide to Diol Metabolites of Unsaturated Fatty Acids in Male Rats. Toxicol Sci 152:309-22
Martinez, Andres; Schnoebelen, Douglas J; Hornbuckle, Keri C (2016) Polychlorinated biphenyl congeners in sediment cores from the Upper Mississippi River. Chemosphere 144:1943-9
Xin, Xing; Senthilkumar, P K; Schnoor, Jerald L et al. (2016) Effects of PCB126 and PCB153 on telomerase activity and telomere length in undifferentiated and differentiated HL-60 cells. Environ Sci Pollut Res Int 23:2173-85
Wangpradit, Orarat; Adamcakova-Dodd, Andrea; Heitz, Katharina et al. (2016) PAMAM dendrimers as nano carriers to investigate inflammatory responses induced by pulmonary exposure of PCB metabolites in Sprague-Dawley rats. Environ Sci Pollut Res Int 23:2128-37
Koh, Wen Xin; Hornbuckle, Keri C; Wang, Kai et al. (2016) Serum polychlorinated biphenyls and their hydroxylated metabolites are associated with demographic and behavioral factors in children and mothers. Environ Int 94:538-45
Osterberg, David; Scammell, Madeleine Kangsen (2016) PCBs in schools--where communities and science come together. Environ Sci Pollut Res Int 23:1998-2002
Herkert, Nicholas J; Martinez, Andres; Hornbuckle, Keri C (2016) A Model Using Local Weather Data to Determine the Effective Sampling Volume for PCB Congeners Collected on Passive Air Samplers. Environ Sci Technol 50:6690-7
Li, Miao; Teesch, Lynn M; Murry, Daryl J et al. (2016) Cytochrome c adducts with PCB quinoid metabolites. Environ Sci Pollut Res Int 23:2148-59
Wangpradit, Orarat; Rahaman, Asif; Mariappan, S V Santhana et al. (2016) Breaking the dogma: PCB-derived semiquinone free radicals do not form covalent adducts with DNA, GSH, and amino acids. Environ Sci Pollut Res Int 23:2138-47
Flor, Susanne; He, Xianran; Lehmler, Hans-Joachim et al. (2016) Estrogenicity and androgenicity screening of PCB sulfate monoesters in human breast cancer MCF-7 cells. Environ Sci Pollut Res Int 23:2186-200

Showing the most recent 10 out of 248 publications