The Analytical Core provides routine and non-routine analytical service to the University of lowa research program. The core provides expert staff, equipment, and instrumentation in line with five Aims.
Specific Aim 1 is the development of analytical and quality control/assurance standards. In close collaboration with the Synthesis Core, which is responsible for acquiring and archiving individual compound standards and preparing stock solutions, the Analytical Core develops the mathematical methods for congener representation, analyzes single congener solutions for purity assessment and quality control, and tests the prepared standard solutions against certified Standard Reference Materials, including PCB calibration mixtures.
Specific Aim 2 is the development of a mixture of PCB congeners for laboratory animal exposure studies. The Analytical Core develop the mathematical methods to design the exposure mixture and tests the prepared mixtures against certified Standard Reference Materials.
Specific Aim 3 is the analysis of environmental and laboratory samples for a suite of PCB congener. The Analytical Core will extract and analyze PCBs on a congener-specific basis: 209 congeners are detected as individual or coeluting sets. We typically achieve 170 congener separations in environmental samples. Applying the primary calibration standard developed under Aim 1, samples are quantified by the internal standard method. Internal Standards and Surrogate Recovery Standards developed under Aim 1 are used to precisely determine PCB concentrations in samples. Methods for analysis on a congener-specific basis are assessed using performance standard injections and analysis of certified Standard Reference Materials. Most analyses are performed on the entire suite of PCB congeners, including laboratory animals exposed to the mixtures described in Aim 2 and including blood from humans and non-laboratory animals. Other samples are analyzed for specific congeners and metabolites in support of investigators focused on congener-specific toxicology.
Specific Aim 4 involves the extraction, identification, and quantification of unknown metabolites. The Analytical Core determines purity of synthesized metabolites and associated analytical standards, then analyzes for hydroxylated PCBs and other PCB metabolites. As requested by the isbrp investigators, the Analytical Core will also train personnel and assist in sampling design and implementation.
Specific Aim 5 is the design and maintenance of a database system. A redundant storage architecture with automatic backup and password protection is used to allow investigators to download data and reports produced by the Analytical Core.
|Liang, Yi; Meggo, Richard; Hu, Dingfei et al. (2014) Enhanced Polychlorinated Biphenyl Removal in a Switchgrass Rhizosphere by Bioaugmentation with Burkholderia xenovorans LB400. Ecol Eng 71:215-222|
|Yang, Shupeng; Shi, Weimin; Hu, Dingfei et al. (2014) In vitro and in vivo metabolite profiling of valnemulin using ultraperformance liquid chromatography-quadrupole/time-of-flight hybrid mass spectrometry. J Agric Food Chem 62:9201-10|
|Salman, Kadhim N; Stuart, Mary A; Schmidt, Jack et al. (2014) The effects of 3,3',4,4'-tetrabromobiphenyl on rats fed diets containing a constant level of copper and varying levels of molybdenum. Environ Sci Pollut Res Int 21:6400-9|
|Tehrani, Rouzbeh; Lyv, Monica M; Van Aken, Benoit (2014) Transformation of hydroxylated derivatives of 2,5-dichlorobiphenyl and 2,4,6-trichlorobiphenyl by Burkholderia xenovorans LB400. Environ Sci Pollut Res Int 21:6346-53|
|Vorrink, Sabine U; Severson, Paul L; Kulak, Mikhail V et al. (2014) Hypoxia perturbs aryl hydrocarbon receptor signaling and CYP1A1 expression induced by PCB 126 in human skin and liver-derived cell lines. Toxicol Appl Pharmacol 274:408-16|
|Ekuase, Edugie J; Lehmler, Hans-Joachim; Robertson, Larry W et al. (2014) Binding interactions of hydroxylated polychlorinated biphenyls (OHPCBs) with human hydroxysteroid sulfotransferase hSULT2A1. Chem Biol Interact 212:56-64|
|Wu, Xianai; Kammerer, Austin; Lehmler, Hans-Joachim (2014) Microsomal oxidation of 2,2',3,3',6,6'-hexachlorobiphenyl (PCB 136) results in species-dependent chiral signatures of the hydroxylated metabolites. Environ Sci Technol 48:2436-44|
|Shen, Hua; Li, Miao; Wang, Bingxuan et al. (2014) Dietary antioxidants (selenium and N-acetylcysteine) modulate paraoxonase 1 (PON1) in PCB 126-exposed rats. Environ Sci Pollut Res Int 21:6384-99|
|Hu, Xin; Adamcakova-Dodd, Andrea; Thorne, Peter S (2014) The fate of inhaled (14)C-labeled PCB11 and its metabolites in vivo. Environ Int 63:92-100|
|Sueyoshi, Tatsuya; Li, Linhao; Wang, Hongbing et al. (2014) Flame retardant BDE-47 effectively activates nuclear receptor CAR in human primary hepatocytes. Toxicol Sci 137:292-302|
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