The goals of the proposed project will be to further develop, biologically validate, employ, and model biological response indicator devices for gauging environmental stressors (BRIDGES). The biological response indicator devices are complementary passive sampling devices (PSD) that bridge environmental exposure and biological response/effect. Because bioavailability processes are embedded in human and ecosystem health risk frameworks, the development of complementary bio-analytical tools that quantitate bioavailability processes is important. Bio-analytical tools that have been rigorously biologically validated using a robust aquatic developmental model system are needed to biologically anchor these analytical approaches. Specifically, we will further develop the BRIDGES tool to assess fate and bioavailability of PAHs (polycyclic aromatic hydrocarbons) in sediments and overlying waters within Superfund, contaminated, urban, and undeveloped field sites and in controlled laboratory studies.
Under Specific Aim 1 we will further develop environmental exposure bio-analytical measurement technologies capable of quantitatively sequestering bioavailable contaminant concentrations.
Under Specific Aim 2 we will utilize the zebrafish developmental model to test the relative potency of PSD extracts from current Superfund, urban, and undeveloped sites.
Under Specific Aim 3 we will develop discriminatory chemical/physical fractions and constructions of PSD extracts from signatory biological responses in the zebrafish model. Develop discriminatory pattern recognition and multivariate regression assessments of co-varying components in PSD extracts and signatory biological responses. Develop a predictive link between biological response/effect and environmental exposures measured by BRIDGES.
Under Specific Aim 4 we will develop discriminatory pattern recognition and multivariate regression assessments of co-varying components in PSD extracts and contaminant source type. The role of environmental exposure and the development of complex human and aquatic health effects require innovative interdisciplinary approaches. We propose to combine two independently developed model systems to further develop, correlate, and validate exposure and response. This interface between environmental exposure and aquatic/human health risk is needed to reliably bridge quantification of exposure and environmental health.
|Knecht, Andrea L; Truong, Lisa; Simonich, Michael T et al. (2016) Developmental benzo[a]pyrene (B[a]P) exposure impacts larval behavior and impairs adult learning in zebrafish. Neurotoxicol Teratol :|
|Bugel, Sean M; Wehmas, Leah C; La Du, Jane K et al. (2016) Phenotype anchoring in zebrafish reveals a potential role for matrix metalloproteinases (MMPs) in tamoxifen's effects on skin epithelium. Toxicol Appl Pharmacol 296:31-41|
|Sadler, Natalie C; Nandhikonda, Premchendar; Webb-Robertson, Bobbie-Jo et al. (2016) Hepatic Cytochrome P450 Activity, Abundance, and Expression Throughout Human Development. Drug Metab Dispos 44:984-91|
|Haggard, Derik E; Noyes, Pamela D; Waters, Katrina M et al. (2016) Phenotypically anchored transcriptome profiling of developmental exposure to the antimicrobial agent, triclosan, reveals hepatotoxicity in embryonic zebrafish. Toxicol Appl Pharmacol 308:32-45|
|Bugel, Sean M; Bonventre, Josephine A; Tanguay, Robert L (2016) Comparative Developmental Toxicity of Flavonoids Using an Integrative Zebrafish System. Toxicol Sci 154:55-68|
|Paulik, L Blair; Smith, Brian W; Bergmann, Alan J et al. (2016) Passive samplers accurately predict PAH levels in resident crayfish. Sci Total Environ 544:782-91|
|Tidwell, Lane G; Allan, Sarah E; O'Connell, Steven G et al. (2016) PAH and OPAH Flux during the Deepwater Horizon Incident. Environ Sci Technol 50:7489-97|
|Garcia, Gloria R; Noyes, Pamela D; Tanguay, Robert L (2016) Advancements in zebrafish applications for 21st century toxicology. Pharmacol Ther 161:11-21|
|Zhang, Guozhu; Roell, Kyle R; Truong, Lisa et al. (2016) A data-driven weighting scheme for multivariate phenotypic endpoints recapitulates zebrafish developmental cascades. Toxicol Appl Pharmacol 314:109-117|
|Truong, Lisa; Bugel, Sean M; Chlebowski, Anna et al. (2016) Optimizing multi-dimensional high throughput screening using zebrafish. Reprod Toxicol 65:139-147|
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