The overall goal of the Structural Biology Core is to facilitate the preparation and molecular characterization of macromolecules of importance to alcohol research. This will be accomplished by assisting researchers in: (1) the cloning or synthesis of cDNAs, cloning of cDNAs into expression vectors, bacterial or yeast fermentation, and the purification of recombinant proteins to homogeneity, (2) the determination of protein or DNA sequence and/or molecular structure by X-ray crystallography or fluorescence, (3) the synthesis of polypeptide antigens for antibody production, and (4) the determination of ligand binding and/or enzyme catalytic rates by stopped-flow kinetics. Researchers in the Center have considerable experience with the above techniques and the purpose of the Core is to provide this expertise to other researchers in the Center (and the alcohol research community at large). The Core will also facilitate access to shared equipment in Center researcher's laboratories, the Biochemistry Biotechnology Facility, and the X-ray Crystallography Facility necessary for preparation of recombinant proteins and analysis of their structure. A major focus of the Center is to elucidate genes that affect alcohol metabolism, alcohol drinking behavior, and alcoholism. It is from structural and biochemical studies of the protein products of these genes that we will gain a greater understanding of the functional effects that polymorphisms may have on alcoholism.

Agency
National Institute of Health (NIH)
Institute
National Institute on Alcohol Abuse and Alcoholism (NIAAA)
Type
Specialized Center (P50)
Project #
5P50AA007611-08
Application #
3726040
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
8
Fiscal Year
1995
Total Cost
Indirect Cost
Name
Indiana University Bloomington
Department
Type
DUNS #
006046700
City
Bloomington
State
IN
Country
United States
Zip Code
47401
McClintick, Jeanette N; McBride, William J; Bell, Richard L et al. (2018) Gene expression changes in the ventral hippocampus and medial prefrontal cortex of adolescent alcohol-preferring (P) rats following binge-like alcohol drinking. Alcohol 68:37-47
Houck, Christa A; Grahame, Nicholas J (2018) Acute drug effects on habitual and non-habitual responding in crossed high alcohol preferring mice. Psychopharmacology (Berl) 235:2167-2175
Ding, Zheng-Ming; Ingraham, Cynthia M; Hauser, Sheketha R et al. (2017) Reduced Levels of mGlu2 Receptors within the Prelimbic Cortex Are Not Associated with Elevated Glutamate Transmission or High Alcohol Drinking. Alcohol Clin Exp Res 41:1896-1906
Linsenbardt, David N; Smoker, Michael P; Janetsian-Fritz, Sarine S et al. (2017) Impulsivity in rodents with a genetic predisposition for excessive alcohol consumption is associated with a lack of a prospective strategy. Cogn Affect Behav Neurosci 17:235-251
Bell, Richard L; Hauser, Sheketha R; Liang, Tiebing et al. (2017) Rat animal models for screening medications to treat alcohol use disorders. Neuropharmacology 122:201-243
Öztürk, Nail Can; Resendiz, Marisol; Öztürk, Hakan et al. (2017) DNA Methylation program in normal and alcohol-induced thinning cortex. Alcohol 60:135-147
Ding, Zheng-Ming; Ingraham, Cynthia M; Rodd, Zachary A et al. (2016) Alcohol drinking increases the dopamine-stimulating effects of ethanol and reduces D2 auto-receptor and group II metabotropic glutamate receptor function within the posterior ventral tegmental area of alcohol preferring (P) rats. Neuropharmacology 109:41-48
Kasten, C R; Frazee, A M; Boehm 2nd, S L (2016) Developing a model of limited-access nicotine consumption in C57Bl/6J mice. Pharmacol Biochem Behav 148:28-37
Zhou, Feng C; Resendiz, Marisol; Lo, Chiao-Ling et al. (2016) Cell-Wide DNA De-Methylation and Re-Methylation of Purkinje Neurons in the Developing Cerebellum. PLoS One 11:e0162063
Qiu, Bin; Bell, Richard L; Cao, Yong et al. (2016) Npy deletion in an alcohol non-preferring rat model elicits differential effects on alcohol consumption and body weight. J Genet Genomics 43:421-30

Showing the most recent 10 out of 253 publications