- NEUROPATHOLOGY CORE The Neuropathology (NP) Core performs diagnostic evaluations and quantitative analyses on brain tissue collected at autopsy of participants in the Mayo Alzheimer Disease Research Center (ADRC). The NP Core provides support to research projects of the ADRC and to other center affiliated investigators. The neuropathologic and genetic data generated by the NP Core is communicated to the Biostatistics and Data Management Core and to the National Alzheimer Coordinating Center. The approach for the NP Core is to: 1. Perform brain autopsies on participants of the Mayo ADRC in a timely fashion and according to protocol. 2. Provide neuropathologic evaluations and collect neuropathologic data using standardized methods for gross dissection and neurohistology. a. Cooperate with Mayo Clinic research neuroradiology in acquisition of post-mortem MRI scans of brains. b. Dissect brains according to a standardized protocol and photograph all sections. Score the severity of cerebrovascular pathology, and measure and sample macroscopic cerebrovascular lesions. c. Use histological methods, including silver stains, thioflavin S fluorescent microscopy and immunohistochemistry for tau and A, to collect standardized pathologic data on all cases. d. Perform immunostaining with antibodies to ?-synuclein on all cases. In cases with Lewy bodies, assign a diagnosis according to the criteria of the Consortium for Dementia with Lewy Bodies. e. Perform immunostaining with antibodies to TDP-43 on all cases and when positive on the screening section, subtype and map the distribution of TDP-43 pathology. f. Arrive at a consensus on clinicopathologic diagnoses at videoconferences held twice a month. g. Provide neuropathological data to the Biostatistics Core and NACC. 3. Store brain tissue and other autopsy-derived materials (e.g., DNA) and provide clinically and pathologically well-characterized tissue samples, DNA or data to ADRC research projects and pilot projects at Mayo Clinic as well as qualified outside investigators. 4. Assist ADRC research projects by providing neuropathologic expertise, tissue or histopathologic services. a. Assist in neuropathologic classification of cases included in the MRI studies of Project 1, which aims to develop an image based diagnostic software program that predicts the underlying pathology. b. Provide autopsy tissue samples of brains of subjects with a range of neurofibrillary pathology in AD and other tauopathies to Project 2 and assist in tissue processing and histologic studies of mouse models. c. Provide brain tissue samples to Project 3 as well as data on cerebral amyloid angiopathy (CAA) with respect to type, distribution, and severity;as well as data on burden of cerebrovascular pathology. 5. Provide genetic screening for ADRC sample sets with respect to existing and newly discovered genes.

Public Health Relevance

- NEUROPATHOLOGY CORE The Neuropathology Core provides a final diagnosis (the gold standard) for the patients enrolled in the study who come to autopsy and it is a repository of data and tissue from human and animal models for research purposes. In addition to contribution to diagnosis and research on Alzheimer's disease, the neuropathology core assists in educational activities of the Alzheimer's Disease Research Center.

National Institute of Health (NIH)
National Institute on Aging (NIA)
Specialized Center (P50)
Project #
Application #
Study Section
Special Emphasis Panel (ZAG1-ZIJ-4 (J1))
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Mayo Clinic, Rochester
United States
Zip Code
Karch, Celeste M; Ezerskiy, Lubov A; Bertelsen, Sarah et al. (2016) Alzheimer's Disease Risk Polymorphisms Regulate Gene Expression in the ZCWPW1 and the CELF1 Loci. PLoS One 11:e0148717
Ma, Li; Allen, Mariet; Sakae, Nobutaka et al. (2016) Expression and processing analyses of wild type and p.R47H TREM2 variant in Alzheimer's disease brains. Mol Neurodegener 11:72
Tosto, Giuseppe; Bird, Thomas D; Bennett, David A et al. (2016) The Role of Cardiovascular Risk Factors and Stroke in Familial Alzheimer Disease. JAMA Neurol 73:1231-1237
Zheng, Honghua; Liu, Chia-Chen; Atagi, Yuka et al. (2016) Opposing roles of the triggering receptor expressed on myeloid cells 2 and triggering receptor expressed on myeloid cells-like transcript 2 in microglia activation. Neurobiol Aging 42:132-41
Staubo, Sara C; Aakre, Jeremiah A; Vemuri, Prashanthi et al. (2016) Mediterranean diet, micronutrients and macronutrients, and MRI measures of cortical thickness. Alzheimers Dement :
Kantarci, Kejal; Lowe, Val J; Lesnick, Timothy G et al. (2016) Early Postmenopausal Transdermal 17β-Estradiol Therapy and Amyloid-β Deposition. J Alzheimers Dis 53:547-56
McCutcheon, Sarah T; Han, Dingfen; Troncoso, Juan et al. (2016) Clinicopathological correlates of depression in early Alzheimer's disease in the NACC. Int J Geriatr Psychiatry 31:1301-1311
Forrester, Sarah N; Gallo, Joseph J; Smith, Gwenn S et al. (2016) Patterns of Neuropsychiatric Symptoms in Mild Cognitive Impairment and Risk of Dementia. Am J Geriatr Psychiatry 24:117-25
Labbé, Catherine; Heckman, Michael G; Lorenzo-Betancor, Oswaldo et al. (2016) MAPT haplotype diversity in multiple system atrophy. Parkinsonism Relat Disord 30:40-5
Nicholson, Alexandra M; Finch, NiCole A; Almeida, Marcio et al. (2016) Prosaposin is a regulator of progranulin levels and oligomerization. Nat Commun 7:11992

Showing the most recent 10 out of 813 publications