The present project proposal, which is divided into two sub-projects focus on the study of the sandfly biological aspects related with its vector potential, and control strategies to decrease leishmaniasis transmission. Sandfly control with residual insecticides, sprayed at the peri and intradomicile level (DDT, Malathion), combined with curtain impregnations (pyrethroides) are proposed for diminishing sandfly populations in endemic foci where transmission is taking place at those levels. Two areas typical of tegumentary and visceral leishmanaisis were selected for the field trials, in which insecticides will be applied on the basis of previous laboratory testing of the compound with colonized sandflies. Evaluation will rely on phlebotomine collections throughout the year as compared with control, untreated. The capacity of Colombia sandflies to transmit Leishmania from different species and subspecies, either in single or mixed infections will be evaluated through artificial infections with promastigotes or amastigotes. Infective potentia will be assessed by evaluating the infection rate, pattern of development, and morphology. Infectivity of egested parasites via forced-feeding and through bite will also be determined. Capacity to harbor and transmit mixed L. braziliensis -L. panamensis infection and L. panamensis - L. chagasi will be explored with similar methodologies.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Specialized Center (P50)
Project #
5P50AI030603-02
Application #
3791588
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
1992
Total Cost
Indirect Cost
Name
University of Valle
Department
Type
DUNS #
City
Cali
State
Country
Colombia
Zip Code
Guarin, Nora; Palma, Gloria I; Pirmez, Claude et al. (2006) Comparative immunohistological analysis of the Montenegro skin test reaction in asymptomatic infection and in acute and chronic cutaneous leishmaniasis. Biomedica 26 Suppl 1:38-48
Travi, Bruno L; Tabares, Carlos Javier; Cadena, Horacio (2006) Leishmania (Viannia) braziliensis infection in two Colombian dogs: a note on infectivity for sand flies and response to treatment. Biomedica 26 Suppl 1:249-53
Rojas, Carlos A; Weigle, Kristen A; Barrera, Lena et al. (2002) Surveillance and screening of American cutaneous leishmaniasis by Colombian primary health care workers using a clinical prediction rule. Trans R Soc Trop Med Hyg 96:405-10
Weigle, Kristen A; Labrada, Luz Angela; Lozano, Caterin et al. (2002) PCR-based diagnosis of acute and chronic cutaneous leishmaniasis caused by Leishmania (Viannia). J Clin Microbiol 40:601-6
Saravia, Nancy Gore; Weigle, Kristen; Navas, Claudia et al. (2002) Heterogeneity, geographic distribution, and pathogenicity of serodemes of Leishmania viannia in Colombia. Am J Trop Med Hyg 66:738-44
Martinez, J E; Valderrama, L; Gama, V et al. (2000) Clonal diversity in the expression and stability of the metastatic capability of Leishmania guyanensis in the golden hamster. J Parasitol 86:792-9
Montoya-Lerma, J; Cadena, H; Segura, I et al. (1999) Association of Lutzomyia columbiana (Diptera: Psychodidae) with a leishmaniasis focus in Colombia due to species of the Leishmania mexicana complex. Mem Inst Oswaldo Cruz 94:277-83
Fernandes, O; Catanho, M P; Segura, I et al. (1999) Minicircle variable region probes for characterization of Leishmania (Viannia) species. J Parasitol 85:563-8
Saravia, N G; Segura, I; Holguin, A F et al. (1998) Epidemiologic, genetic, and clinical associations among phenotypically distinct populations of Leishmania (Viannia) in Colombia. Am J Trop Med Hyg 59:86-94
Osorio, Y; Gonzalez, S J; Gama, V L et al. (1998) Reinfection in American cutaneous leishmaniasis: evaluation of clinical outcomes in the hamster model. Mem Inst Oswaldo Cruz 93:353-6

Showing the most recent 10 out of 34 publications