The development of obesity. Insulin resistance, and metabolic syndrome Involves dysfunction of multiple organ systems and occurs In a complex and progressive fashion. In the initial funding cycle, small animal metabolic phenotyping was used to establish that two of the botanical extracts under study at our Center and (i.e. artemlsia dracunulus L., Incarnatin) ameliorated the developmental pathology ofthis process. More Importantly, this experimental strategy provided key insights regarding the tissue sites where beneficial effects were realized. The evolution of our experimental approach in the next cycle involves combining small animal metabolic phenotyping with the analytical power of metabolomic profiling to obtain the biochemical phenotypes that will provide insights Into the pathogenesis of Insulin resistance, and the mechanisms engaged by botanical extracts to ameliorate the progression. The Integrative Biology Core will combine state-of-the-art in vivo metabolic phenotyping with detailed ex vivo proteomic and metabolomic profiling of serum and tissue samples to provide highly Integrated metabolic signatures of the pathophysiology of insulin resistance and its resolution by botanical extracts. The Integrative Biology Core will be located at the Pennington and will be comprised of 2 interactive components: the Animal Research Subcore and the Analytical Chemistry Subcore, comprised of a Clinical Chemistry/Stable Isotope component and a Proteomic/Metabolomic component. The Integrative Biology Core will function Interactively with the Project leaders, the Administrative Core and the Botanical Core in all phases of experimental planning, execution, analysis, and interpretation. The overall goal of the IBC is to bring the combined expertise of all elements of the Botanical Research Center to bear upon our collective efforts to provide novel insights Into these mechanisms. We will use rodent models of obesity/diabetes to evaluate the mechanism of botanical extracts to ameliorate the development of this pathology, conducting detailed in vivo metabolic phenotyping to establish efficacy and guide timing of tissue harvest for rigorous ex vivo metabolomic profiling. The resulting biochemical signatures will be used to gain Insights Into the mechanism of action of botanical extracts.
The ability to obtain metabolic snapshots of substrate fluxes through metabolic pathways that integrate carbohydrate and lipid metabolism affords us an exciting opportunity to gain new molecular and physiologic insights Into the successful resolution of pathogenic mechanisms by our selected botanicals.
|Knott, Eric J; Richard, Allison J; Mynatt, Randall L et al. (2017) Fenugreek supplementation during high-fat feeding improves specific markers of metabolic health. Sci Rep 7:12770|
|Cefalu, William T; Buse, John B; Tuomilehto, Jaakko et al. (2017) Response to Comment on Cefalu et al. Update and Next Steps for Real-World Translation of Interventions for Type 2 Diabetes Prevention: Reflections From a Diabetes Care Editors' Expert Forum. Diabetes Care 2016;39:1186-1201. Diabetes Care 40:e23-e24|
|Cefalu, William T; Boulton, Andrew J M; Tamborlane, William V et al. (2017) Diabetes Care: ""Taking It to the Limit One More Time"". Diabetes Care 40:3-6|
|Johnson, William D (2017) Author's response. Ann Allergy Asthma Immunol 118:525|
|Armas, Isabel; Pogrebnyak, Natalia; Raskin, Ilya (2017) A rapid and efficient in vitro regeneration system for lettuce (Lactuca sativa L.). Plant Methods 13:58|
|Rebello, Candida J; Greenway, Frank L; Johnson, William D et al. (2017) Fucoxanthin and Its Metabolite Fucoxanthinol Do Not Induce Browning in Human Adipocytes. J Agric Food Chem 65:10915-10924|
|Losso, Jack N; Finley, John W; Karki, Namrata et al. (2017) Pilot Study of the Tart Cherry Juice for the Treatment of Insomnia and Investigation of Mechanisms. Am J Ther :|
|Cefalu, William T; Buse, John B; Tuomilehto, Jaakko et al. (2016) Update and Next Steps for Real-World Translation of Interventions for Type 2 Diabetes Prevention: Reflections From a Diabetes Care Editors' Expert Forum. Diabetes Care 39:1186-201|
|Cefalu, William T; Boulton, Andrew J M; Tamborlane, William V et al. (2016) Diabetes Care: ""Lagniappe"" and ""Seeing Is Believing""! Diabetes Care 39:1069-71|
|Moses, Robert G; Cefalu, William T (2016) Considerations in the Management of Gestational Diabetes Mellitus: ""You Are What Your Mother Ate!"". Diabetes Care 39:13-5|
Showing the most recent 10 out of 161 publications