Abstract

The Peptide Synthesis and Protein and Analysis Core will provide service and advice to RCOB investigators in the area of peptide synthesis, protein sequencing and hexosamine and amino acid analysis. Protein Microsequencing is performed on a Porton Model PI 2090E peptide microsequencing system using on-line HPLC for identification of PTH-amino acids. Identification of the residue released at each cycle is aided by computer software which overlays or subtracts successive PTH-amino acid separations. Peptide Synthesis is performed on a Rainin Model P53 using FMOC solid phase automated peptide chemistry. Peptide are cleaved from the resin support and the purity judged from a reverse-phase HPLC separation. Hexosamine and Amino Acid Analysis is done using the Waters PicoTag system. The Core will be used to a major extent by Projects 3 and 7 and occasionally by Project 6. Equipment for the core, Porton Model PI 2090E peptide microsequencing system, Rainin Model P53 peptide synthesizer and Perkin-Elmer (Series 4) HPLC have recently been acquired from other funding sources.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Specialized Center (P50)
Project #
3P50DE008228-10S3
Application #
6104734
Study Section
Project Start
1998-12-01
Project End
2000-04-30
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
10
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
University of Alabama Birmingham
Department
Type
DUNS #
004514360
City
Birmingham
State
AL
Country
United States
Zip Code
35294

  Publications

Windsor, L Jack; Steele, Darin L (2010) Expression of recombinant matrix metalloproteinases in Escherichia coli. Methods Mol Biol 622:67-81
Katz, Jannet; Yang, Qiu-Bo; Zhang, Ping et al. (2002) Hydrolysis of epithelial junctional proteins by Porphyromonas gingivalis gingipains. Infect Immun 70:2512-8
Smith, P D; Smythies, L E; Mosteller-Barnum, M et al. (2001) Intestinal macrophages lack CD14 and CD89 and consequently are down-regulated for LPS- and IgA-mediated activities. J Immunol 167:2651-6
Aznavoorian, S; Moore, B A; Alexander-Lister, L D et al. (2001) Membrane type I-matrix metalloproteinase-mediated degradation of type I collagen by oral squamous cell carcinoma cells. Cancer Res 61:6264-75
Hirschfeld, M; Weis, J J; Toshchakov, V et al. (2001) Signaling by toll-like receptor 2 and 4 agonists results in differential gene expression in murine macrophages. Infect Immun 69:1477-82
Windsor, L J; Steele, D L (2001) Expression of recombinant matrix metalloproteinases in Escherichia coli. Methods Mol Biol 151:191-205
Caterina, J J; Shi, J; Kozak, C A et al. (2000) Characterization, expression analysis and chromosomal mapping of mouse matrix metalloproteinase-19 (MMP-19). Mol Biol Rep 27:73-9
Foster, K W; Frost, A R; McKie-Bell, P et al. (2000) Increase of GKLF messenger RNA and protein expression during progression of breast cancer. Cancer Res 60:6488-95
Caterina, J; Shi, J; Sun, X et al. (2000) Cloning, characterization, and expression analysis of mouse enamelysin. J Dent Res 79:1697-703
Caterina, J J; Yamada, S; Caterina, N C et al. (2000) Inactivating mutation of the mouse tissue inhibitor of metalloproteinases-2(Timp-2) gene alters proMMP-2 activation. J Biol Chem 275:26416-22

Showing the most recent 10 out of 144 publications