Abstract

The Molecular Biology and Protein Analysis Core is a major resource for the Center because it will provide needed reagents and technological services, as well as transferring methodology and as a training center. Specific technological services supported by the Core will be: (1) synthesis of oligonucleotides for use as hybridization probes, DNA sequencing and polymerase chain reaction primers and coding sequences in plasmid expression vectors, (2) automated fluorescent dye-label DNA sequencing, (3) amino acid composition analysis of purified proteins and peptides, (4) N-terminal microsequencing of proteins and peptides for determination of primary sequence, (5) development of expression vector systems, and (6) high resolution chromatography for separation of peptides, purification of native proteins, and isolation of DNA restriction fragments free from contaminating RNA.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Specialized Center (P50)
Project #
5P50DE008239-10
Application #
6238414
Study Section
Project Start
1996-12-01
Project End
2000-04-30
Budget Start
Budget End
Support Year
10
Fiscal Year
1997
Total Cost
Indirect Cost

  Institution

Name
University of Pennsylvania
Department
Type
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104

  Publications

Peng, T; Ponce-de-Leon, M; Jiang, H et al. (1998) The gH-gL complex of herpes simplex virus (HSV) stimulates neutralizing antibody and protects mice against HSV type 1 challenge. J Virol 72:65-72
Bashir, M M; Abrams, W R; Tucker, T et al. (1998) Molecular cloning and characterization of the bovine and human tuftelin genes. Connect Tissue Res 39:13-24;discussion 63-7
Karakelian, D; Lear, J D; Lally, E T et al. (1998) Characterization of Actinobacillus actinomycetemcomitans leukotoxin pore formation in HL60 cells. Biochim Biophys Acta 1406:175-87
Taichman, N S; Cruchley, A T; Fletcher, L M et al. (1998) Vascular endothelial growth factor in normal human salivary glands and saliva: a possible role in the maintenance of mucosal homeostasis. Lab Invest 78:869-75
Peng, T; Ponce de Leon, M; Novotny, M J et al. (1998) Structural and antigenic analysis of a truncated form of the herpes simplex virus glycoprotein gH-gL complex. J Virol 72:6092-103
Nicola, A V; Peng, C; Lou, H et al. (1997) Antigenic structure of soluble herpes simplex virus (HSV) glycoprotein D correlates with inhibition of HSV infection. J Virol 71:2940-6
Bashir, M M; Abrams, W R; Rosenbloom, J (1997) Molecular cloning and characterization of the bovine tuftelin gene. Arch Oral Biol 42:489-96
Jenkinson, H F; Demuth, D R (1997) Structure, function and immunogenicity of streptococcal antigen I/II polypeptides. Mol Microbiol 23:183-90
Lally, E T; Kieba, I R; Sato, A et al. (1997) RTX toxins recognize a beta2 integrin on the surface of human target cells. J Biol Chem 272:30463-9
Whitbeck, J C; Peng, C; Lou, H et al. (1997) Glycoprotein D of herpes simplex virus (HSV) binds directly to HVEM, a member of the tumor necrosis factor receptor superfamily and a mediator of HSV entry. J Virol 71:6083-93

Showing the most recent 10 out of 68 publications