The Neuroendocrine Assay Core will provide the UCLA Center for Neurovisceral Sciences and Women's Health with state of the art resources and expertise related to the measurement of neuroendocrine mediators involved in central and peripheral stress signaling pathways. Additional services related to measurements of the HPA-axis activation in the blood and fat tissue have been added to accommodate changes in the needs of the projects in renewal application and personnel have been changed to reflect these requirements. The Neuroendocrine Assay Core will be directed by Dr. Iordanis Karagiannidis, PhD and co-directed by Dr. Charalabos Pothoulakis, MD and will be an independent Core lab within the next funding period. The Core is equipped with EchoMRI, plate readers. Real time PCR and the high-throughput Bio-Plex 3D Suspension array System. Dr. Karagiannidis has extensive training in molecular biology techniques as well as the use of these equipment. He also has considerable expertise on adipocyte biology throughout his career with emphasis on adipocyte differentiation and neuropeptide effects on adipocyte-associated inflammatory responses as they associate to IBD and in separate projects on the development of insulin resistance. Dr. Pothoulakis is a senior scientist in the field of intestinal inflammation and a leading expert in neuropeptide research. He will assist with counseling on the direction of the studies and the proposition of potential targets for analysis. Dimitris Stavrakis has been working as a student volunteer and as a technician for the last 2 years and is very capable with real time PCR, ELISA, western immunoblotting, adipocyte culture techniques and RNA, DNA and protein isolation. He will be assisting with the analysis of Core samples. In addition to the analysis of serum samples, cellular responses and RNA expression, the Core members will work closely with other members ofthe program to provide and receive consultation as well as advise on the performance of experiments. Drs. Pothoulakis and Karagiannidis have already had extensive collaborations with several other Program participants which produced a number of publications. The Core will also provide training as part of the Career Development Program, and participate at all levels in Center operations.
The Neuroendocrine Core will perform key measurements that will help identify biomarkers relevant to increased stress responsiveness seen in IBS and provide molecular explanations for excessive abdominal fat accumulation and brain changes observed in IBS and animal models of the disease. Information from measurements performed in this Core may identify novel targets for therapeutic interventions in IBS.
|Videlock, Elizabeth J; Mahurkar-Joshi, Swapna; Hoffman, Jill M et al. (2018) Sigmoid colon mucosal gene expression supports alterations of neuronal signaling in irritable bowel syndrome with constipation. Am J Physiol Gastrointest Liver Physiol 315:G140-G157|
|Bonfiglio, Ferdinando; Zheng, Tenghao; Garcia-Etxebarria, Koldo et al. (2018) Female-Specific Association Between Variants on Chromosome 9 and Self-Reported Diagnosis of Irritable Bowel Syndrome. Gastroenterology 155:168-179|
|Park, S H; Naliboff, B D; Shih, W et al. (2018) Resilience is decreased in irritable bowel syndrome and associated with symptoms and cortisol response. Neurogastroenterol Motil 30:|
|Martin, Clair R; Osadchiy, Vadim; Kalani, Amir et al. (2018) The Brain-Gut-Microbiome Axis. Cell Mol Gastroenterol Hepatol 6:133-148|
|Addante, Raymond; Naliboff, Bruce; Shih, Wendy et al. (2018) Predictors of Health-related Quality of Life in Irritable Bowel Syndrome Patients Compared With Healthy Individuals. J Clin Gastroenterol :|
|Gupta, Arpana; Woodworth, Davis C; Ellingson, Benjamin M et al. (2018) Disease-Related Microstructural Differences in the Brain in Women With Provoked Vestibulodynia. J Pain 19:528.e1-528.e15|
|Gupta, Arpana; Mayer, Emeran A; Labus, Jennifer S et al. (2018) Sex Commonalities and Differences in Obesity-Related Alterations in Intrinsic Brain Activity and Connectivity. Obesity (Silver Spring) 26:340-350|
|Tache, Yvette; Larauche, Muriel; Yuan, Pu-Qing et al. (2018) Brain and Gut CRF Signaling: Biological Actions and Role in the Gastrointestinal Tract. Curr Mol Pharmacol 11:51-71|
|Hoffman, Jill M; Sideri, Aristea; Ruiz, Jonathan J et al. (2018) Mesenteric Adipose-derived Stromal Cells From Crohn's Disease Patients Induce Protective Effects in Colonic Epithelial Cells and Mice With Colitis. Cell Mol Gastroenterol Hepatol 6:1-16|
|Fang, Kai; Law, Ivy Ka Man; Padua, David et al. (2018) MicroRNA-31-3p Is Involved in Substance P (SP)-Associated Inflammation in Human Colonic Epithelial Cells and Experimental Colitis. Am J Pathol 188:586-599|
Showing the most recent 10 out of 219 publications