The CCHMC PCEN Gene Expression Core (Core A) provides essential NexGen sequencing based RNA- Seq services, as well as more conventional microarray based gene expression technology. This is coupled with an integrative bioinformatics analysis capacity for investigators. Core A will provide consultation and collaboration with respect to experimental design, data generation and data analysis. Core A will facilitate planning and execution of mRNA, miRNA and total RNA (including noncoding RNA) gene expression analysis using a variety of technologies, including multiple library preparation and target amplification protocols for RNA-Seq and microarrays, using the lllumina HiSeq 2000 for NexGen analysis, and both Affymetrix and lllumina platforms for microarray analysis. In addition we offer capacity for Chip-Chip, Chip- Seq RNA splicing and promoter profiling analyses. Data from these technologies are fully interfaced to their corresponding bioinformatics analysis platforms, custom developed databases for sample and tracking, and a suite of advanced analysis softwares for DNA, genomic and transcriptomic analyses are readily accessible to the Center's investigators through a high performance computing environment Core A will dramatically enhance the ability ofthe members ofthe PCEN to make use of global gene expression technologies in their research projects. In addition the core will provide dedicated support for data analysis, collaboration, and educational activities for both established and junior investigators, as well as trainees.
Aim 1 will empower Center members by providing access to integrated state ofthe art gene expression technologies with which to apply genomics methods to accelerate molecular nephrology research.
Aim 2 will educate, assist, and iriiprove the use of global genomics approaches, infrastructure and specialized tools for the enhancement of research productivity and discovery by Center investigators.
Aim 3 will develop and disseminate specialized data sets and the use of advanced analytic techniques and/or protocols to facilitate translation of research data to accelerate basic and applied research and its translation to improved clinical care.

Public Health Relevance

Pediatric kidney diseases due to acute kidney injury, focal segmental glomerulosclerosis, and lupus nephritis contribute to an enormous major impact on the U.S. public health and a major financial burden. The Gene Expression Core ofthis Center of Excellence in Nephrology will provide critical genomic tools for miiltiple investigators to advance their studies on these three disease states to change their dismal outcomes

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Specialized Center (P50)
Project #
1P50DK096418-01
Application #
8397957
Study Section
Special Emphasis Panel (ZDK1-GRB-G (M3))
Project Start
Project End
Budget Start
2012-09-21
Budget End
2013-08-31
Support Year
1
Fiscal Year
2012
Total Cost
$152,507
Indirect Cost
$52,829
Name
Cincinnati Children's Hospital Medical Center
Department
Type
DUNS #
071284913
City
Cincinnati
State
OH
Country
United States
Zip Code
45229
Wang, Zhu; Ma, Shuangge; Zappitelli, Michael et al. (2016) Penalized count data regression with application to hospital stay after pediatric cardiac surgery. Stat Methods Med Res 25:2685-2703
Devarajan, Prasad; Jefferies, John Lynn (2016) PROGRESSION OF CHRONIC KIDNEY DISEASE AFTER ACUTE KIDNEY INJURY. Prog Pediatr Cardiol 41:33-40
Bertram, John F; Goldstein, Stuart L; Pape, Lars et al. (2016) Kidney disease in children: latest advances and remaining challenges. Nat Rev Nephrol 12:182-91
Soto, Karina; Campos, Pedro; Pinto, Iola et al. (2016) The risk of chronic kidney disease and mortality are increased after community-acquired acute kidney injury. Kidney Int 90:1090-1099
Abulaban, K M; Song, H; Zhang, X et al. (2016) Predicting decline of kidney function in lupus nephritis using urine biomarkers. Lupus 25:1012-8
Cooper, David S; Claes, Donna; Goldstein, Stuart L et al. (2016) Follow-Up Renal Assessment of Injury Long-Term After Acute Kidney Injury (FRAIL-AKI). Clin J Am Soc Nephrol 11:21-9
Jefferies, John Lynn; Devarajan, Prasad (2016) Early detection of acute kidney injury after pediatric cardiac surgery. Prog Pediatr Cardiol 41:9-16
Ma, Qing; Devarajan, Shiva R; Devarajan, Prasad (2016) Amelioration of cisplatin-induced acute kidney injury by recombinant neutrophil gelatinase-associated lipocalin. Ren Fail 38:1476-1482
Erkan, Elif; Zhao, Xueheng; Setchell, Kenneth et al. (2016) Distinct urinary lipid profile in children with focal segmental glomerulosclerosis. Pediatr Nephrol 31:581-8
Mina, Rina; Abulaban, Khalid; Klein-Gitelman, Marisa S et al. (2016) Validation of the Lupus Nephritis Clinical Indices in Childhood-Onset Systemic Lupus Erythematosus. Arthritis Care Res (Hoboken) 68:195-202

Showing the most recent 10 out of 66 publications