The Cincinnati Children's Hospital Medical Center Biomarker Core (Core C) with provide support for all aspects of biomarker research to the Pediatric Centers of Excellence in Nephrology. These services range from specimen handling, biofluid profiling and discovery, to assay development and biomarker validation, to commercialization. The overarching goal of Biomarker Core C is to provide an innovative service for the seamless discovery, translation, validation, and commercialization of novel biomarkers and therapeutic targets for human diseases, in collaboration with other cores. The Biomarker Core, founded by Prasad Devarajan, MD, and co-directed by Michael Bennett, PhD, is housed in the Division of Nephrology and Hypertension at Cincinnati Children's Research Foundation. Through collaborative and fee-for-service arrangements, the lab has grown into a wide-ranging service and research provider for clinicians and investigators across the globe. Over the past three years, we have performed well over 200,000 assays for AKI and CKD biomarkers for investigators worldwide. Dr. Bennett has a broad background in cell biology, molecular biology and biochemisty. He was recruited to join Dr. Devarajan's team 5 years ago to direct the Biomarker Core and supervise the research operations of the Nephrology Clinical Laboratory. The seamless connection to the CAP and CLIA certified clinical laboratory provides the Biomarker Core access to a diverse menu of advanced clinical assays on high throughput standardized clinical laboratory platforms. Several of the research projects in this application (Goldstein, Brunner, Basu, Jodele,) require the services of the Biomarker Core to fulfill their Aims. To serve the needs of this team of investigators, the Biomarker Core will focus on these two specific aims: (1) To provide high throughput Clinical Biofluid Profiling Services using SELDI-TOF (list investigators who will use this), and (2) To provide services pertaining to Biomarker measurement and validation (including Design of ELISA and Western Blots, Measurement of markers of AKI and CKD, as well as consultation for biomarker statistics and clinical trials design).

Public Health Relevance

Pediatric kidney diseases due to acute kidney injury, focal segmental glomerulosclerosis, and lupus nephritis contribute to an enormous major impact on the U.S. public health and a major financial burden. The Biomarker Core of this Center of Excellence will provide critical biomarker discovery and validation expertise for multiple investigators to advance studies on these three disease states to change their dismal outcome

National Institute of Health (NIH)
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Specialized Center (P50)
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Special Emphasis Panel (ZDK1)
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Nehus, Edward; Kaddourah, Ahmad; Bennett, Michael et al. (2017) Subclinical Kidney Injury in Children Receiving Nonsteroidal Anti-Inflammatory Drugs After Cardiac Surgery. J Pediatr 189:175-180
Varnell Jr, Charles D; Goldstein, Stuart L; Devarajan, Prasad et al. (2017) Impact of Near Real-Time Urine Neutrophil Gelatinase-Associated Lipocalin Assessment on Clinical Practice. Kidney Int Rep 2:1243-1249
Bennett, Michael R; Pleasant, LaTawnya; Haffner, Christopher et al. (2017) A Novel Biomarker Panel to Identify Steroid Resistance in Childhood Idiopathic Nephrotic Syndrome. Biomark Insights 12:1177271917695832
Turnier, Jessica L; Fall, Ndate; Thornton, Sherry et al. (2017) Urine S100 proteins as potential biomarkers of lupus nephritis activity. Arthritis Res Ther 19:242
Garimella, Pranav S; Jaber, Bertrand L; Tighiouart, Hocine et al. (2017) Association of Preoperative Urinary Uromodulin with AKI after Cardiac Surgery. Clin J Am Soc Nephrol 12:10-18
Garimella, Pranav S; Bartz, Traci M; Ix, Joachim H et al. (2017) Urinary Uromodulin and Risk of Urinary Tract Infections: The Cardiovascular Health Study. Am J Kidney Dis 69:744-751
Gulati, G; Bennett, M R; Abulaban, K et al. (2017) Prospective validation of a novel renal activity index of lupus nephritis. Lupus 26:927-936
Devarajan, Prasad; Basu, Rajit K (2017) Sepsis-associated acute kidney injury - is it possible to move the needle against this syndrome? J Pediatr (Rio J) 93:1-3
Kamal, Fadia A; Travers, Joshua G; Schafer, Allison E et al. (2017) G Protein-Coupled Receptor-G-Protein ??-Subunit Signaling Mediates Renal Dysfunction and Fibrosis in Heart Failure. J Am Soc Nephrol 28:197-208
Pleasant, LaTawnya; Ma, Qing; Devarajan, Mahima et al. (2017) Increased susceptibility to structural acute kidney injury in a mouse model of presymptomatic cardiomyopathy. Am J Physiol Renal Physiol 313:F699-F705

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