This core will coordinate and provide: 1. human cell lines ((volunteer neutrophils, monocytes, epithelial cells, (pulmonary microvascular endoithelial cells, hMVEC);2. imaging and optical experiment facility (FRET, 3D reconstruction, multichannel imaging and cytometry);3. Mass spectroscopic protein analysis and 4. efficient use of a perishable inventory of labeled primary and secondary antibodies. At present the Core is equipped for full tissue culture capabilities, two digital deconvoluting microscopes equipped to perform live or fixed cell 3D imaging in upto 4 color channels;Beckman Coulter flow cytometer for uptpo 5 fluorophores, 3 mass spectrometers with supporting 1 and 2 gel elctrophoresis equipment, and robotic spot pickers. The Core is staffed by personnel who are highly experienced in all aspects of primary human cell culture, various imaging methodologies and proteomics. The core will actively move into the future (2-4 years), 1. by linking data to national databases (primarily the GEO project) and 2. expanding its own repertoire of analytical methods before the need arises, 3. Linking to other core analytical efforts (NMR, MS, microarrays) within the region.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Specialized Center (P50)
Project #
5P50GM049222-19
Application #
8499334
Study Section
Special Emphasis Panel (ZGM1-PPBC-5)
Project Start
Project End
Budget Start
2013-06-01
Budget End
2014-05-31
Support Year
19
Fiscal Year
2013
Total Cost
$426,423
Indirect Cost
$147,715
Name
University of Colorado Denver
Department
Type
DUNS #
041096314
City
Aurora
State
CO
Country
United States
Zip Code
80045
Lawson, Peter J; Moore, Hunter B; Moore, Ernest E et al. (2017) Preoperative thrombelastography maximum amplitude predicts massive transfusion in liver transplantation. J Surg Res 220:171-175
Moore, Frederick A; Moore, Ernest E; Billiar, Timothy R et al. (2017) The role of NIGMS P50 sponsored team science in our understanding of multiple organ failure. J Trauma Acute Care Surg 83:520-531
Stettler, Gregory R; Moore, Ernest E; Moore, Hunter B et al. (2017) Platelet adenosine diphosphate receptor inhibition provides no advantage in predicting need for platelet transfusion or massive transfusion. Surgery 162:1286-1294
Moore, Hunter B; Moore, Ernest E; Chapman, Michael P et al. (2017) Viscoelastic Tissue Plasminogen Activator Challenge Predicts Massive Transfusion in 15 Minutes. J Am Coll Surg 225:138-147
Clendenen, Nathan; Nunns, Geoffrey R; Moore, Ernest E et al. (2017) Hemorrhagic shock and tissue injury drive distinct plasma metabolome derangements in swine. J Trauma Acute Care Surg 83:635-642
Sauaia, Angela; Moore, Frederick A; Moore, Ernest E (2017) Postinjury Inflammation and Organ Dysfunction. Crit Care Clin 33:167-191
Slaughter, Anne L; Nunns, Geoffrey R; D'Alessandro, Angelo et al. (2017) The Metabolopathy of Tissue Injury, Hemorrhagic Shock and Resuscitation in a Rat Model. Shock :
Silliman, Christopher C; Kelher, Marguerite R; Khan, Samina Y et al. (2017) Supernatants and lipids from stored red blood cells activate pulmonary microvascular endothelium through the BLT2 receptor and protein kinase C activation. Transfusion 57:2690-2700
Reisz, Julie A; Slaughter, Anne L; Culp-Hill, Rachel et al. (2017) Red blood cells in hemorrhagic shock: a critical role for glutaminolysis in fueling alanine transamination in rats. Blood Adv 1:1296-1305
D?Alessandro, Angelo; Moore, Hunter B; Moore, Ernest E et al. (2017) Plasma succinate is a predictor of mortality in critically injured patients. J Trauma Acute Care Surg 83:491-495

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