This core will coordinate and provide: 1. human cell lines ((volunteer neutrophils, monocytes, epithelial cells, (pulmonary microvascular endoithelial cells, hMVEC);2. imaging and optical experiment facility (FRET, 3D reconstruction, multichannel imaging and cytometry);3. Mass spectroscopic protein analysis and 4. efficient use of a perishable inventory of labeled primary and secondary antibodies. At present the Core is equipped for full tissue culture capabilities, two digital deconvoluting microscopes equipped to perform live or fixed cell 3D imaging in upto 4 color channels;Beckman Coulter flow cytometer for uptpo 5 fluorophores, 3 mass spectrometers with supporting 1 and 2 gel elctrophoresis equipment, and robotic spot pickers. The Core is staffed by personnel who are highly experienced in all aspects of primary human cell culture, various imaging methodologies and proteomics. The core will actively move into the future (2-4 years), 1. by linking data to national databases (primarily the GEO project) and 2. expanding its own repertoire of analytical methods before the need arises, 3. Linking to other core analytical efforts (NMR, MS, microarrays) within the region.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Specialized Center (P50)
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Special Emphasis Panel (ZGM1-PPBC-5)
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University of Colorado Denver
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Brown, Joshua B; Cohen, Mitchell J; Minei, Joseph P et al. (2015) Pretrauma center red blood cell transfusion is associated with reduced mortality and coagulopathy in severely injured patients with blunt trauma. Ann Surg 261:997-1005
Stahel, Philip F; Moore, Ernest E (2014) Peer review for biomedical publications: we can improve the system. BMC Med 12:179
Silliman, C C; Bercovitz, R S; Khan, S Y et al. (2014) Antibodies to the HLA-A2 antigen prime neutrophils and serve as the second event in an in vitro model of transfusion-related acute lung injury. Vox Sang 107:76-82
Chin, Theresa L; Moore, Ernest E; Moore, Hunter B et al. (2014) A principal component analysis of postinjury viscoelastic assays: clotting factor depletion versus fibrinolysis. Surgery 156:570-7
Wohlauer, M; Kobeiter, H; Desgranges, P et al. (2014) Inferior Mesenteric Artery Stenting as a Novel Treatment for Chronic Mesenteric Ischemia in Patients with an Occluded Superior Mesenteric Artery and Celiac Trunk. Eur J Vasc Endovasc Surg 27:e21-e23
Stringham, John R; Moore, Ernest E; Gamboni, Fabia et al. (2014) Mesenteric lymph diversion abrogates 5-lipoxygenase activation in the kidney following trauma and hemorrhagic shock. J Trauma Acute Care Surg 76:1214-21
Jones, Edward L; Stovall, Robert T; Jones, Teresa S et al. (2014) Intra-abdominal injury following blunt trauma becomes clinically apparent within 9 hours. J Trauma Acute Care Surg 76:1020-3
Dzieciatkowska, Monika; D'Alessandro, Angelo; Moore, Ernest E et al. (2014) Lymph is not a plasma ultrafiltrate: a proteomic analysis of injured patients. Shock 42:485-98
Gonzalez, E; Moore, E E; Moore, H B et al. (2014) Trauma-Induced Coagulopathy: An Institution's 35 Year Perspective on Practice and Research. Scand J Surg 103:89-103
Moore, Ernest E; Chin, Theresa L; Chapman, Michael C et al. (2014) Plasma first in the field for postinjury hemorrhagic shock. Shock 41 Suppl 1:35-8

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