directed by Peijun Zhang, with collaborative effort from Hanspeter Winkler (Florida State U). Service Mission: The CryoEM Core will: 1) characterize structural features of HIV and host protein complexes by negative staining to determine their suitability for further structural study;2) determine the structures of complexes/assemblies deemed suitable;3) develop methods for single particle cryoEM and cryoET to analyze structural heterogeneity.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Specialized Center (P50)
Project #
5P50GM082251-07
Application #
8546403
Study Section
Special Emphasis Panel (ZRG1-AARR-K)
Project Start
Project End
Budget Start
2013-09-01
Budget End
2014-08-31
Support Year
7
Fiscal Year
2013
Total Cost
$283,685
Indirect Cost
$96,434
Name
University of Pittsburgh
Department
Type
DUNS #
004514360
City
Pittsburgh
State
PA
Country
United States
Zip Code
15213
Zhao, Gongpu; Zhang, Peijun (2014) CryoEM analysis of capsid assembly and structural changes upon interactions with a host restriction factor, TRIM5?. Methods Mol Biol 1087:13-28
Jung, Jinwon; Byeon, In-Ja L; DeLucia, Maria et al. (2014) Binding of HIV-1 Vpr protein to the human homolog of the yeast DNA repair protein RAD23 (hHR23A) requires its xeroderma pigmentosum complementation group C binding (XPCB) domain as well as the ubiquitin-associated 2 (UBA2) domain. J Biol Chem 289:2577-88
Henning, Matthew S; Dubose, Brittany N; Burse, Mallori J et al. (2014) In vivo functions of CPSF6 for HIV-1 as revealed by HIV-1 capsid evolution in HLA-B27-positive subjects. PLoS Pathog 10:e1003868
Vorontsov, Ivan I; Wu, Ying; DeLucia, Maria et al. (2014) Mechanisms of allosteric activation and inhibition of the deoxyribonucleoside triphosphate triphosphohydrolase from Enterococcus faecalis. J Biol Chem 289:2815-24
Sharaf, Naima G; Poliner, Eric; Slack, Ryan L et al. (2014) The p66 immature precursor of HIV-1 reverse transcriptase. Proteins 82:2343-52
Maertens, Goedele N; Cook, Nicola J; Wang, Weifeng et al. (2014) Structural basis for nuclear import of splicing factors by human Transportin 3. Proc Natl Acad Sci U S A 111:2728-33
Hou, Guangjin; Gupta, Rupal; Polenova, Tatyana et al. (2014) A Magic-Angle Spinning NMR Method for the Site-Specific Measurement of Proton Chemical-Shift Anisotropy in Biological and Organic Solids. Isr J Chem 54:171-183
Suiter, Christopher L; Paramasivam, Sivakumar; Hou, Guangjin et al. (2014) Sensitivity gains, linearity, and spectral reproducibility in nonuniformly sampled multidimensional MAS NMR spectra of high dynamic range. J Biomol NMR 59:57-73
Mitra, Mithun; Hercík, Kamil; Byeon, In-Ja L et al. (2014) Structural determinants of human APOBEC3A enzymatic and nucleic acid binding properties. Nucleic Acids Res 42:1095-110
Ishima, Rieko (2014) A probe to monitor performance of ¹?N longitudinal relaxation experiments for proteins in solution. J Biomol NMR 58:113-22

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