CryoEM Core,directed by Peijun Zhang, with collaborative effort from Hanspeter Winkler (Florida State U). Service Mission: The CryoEM Core will: 1) characterize structural features of HIV and host protein complexes by negative staining to determine their suitability for further structural study;2) determine the structures of complexes/assemblies deemed suitable;3) develop methods for single particle cryoEM and cryoET to analyze structural heterogeneity.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Specialized Center (P50)
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Special Emphasis Panel (ZRG1-AARR-K)
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University of Pittsburgh
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Zhao, Gongpu; Zhang, Peijun (2014) CryoEM analysis of capsid assembly and structural changes upon interactions with a host restriction factor, TRIM5?. Methods Mol Biol 1087:13-28
Jung, Jinwon; Byeon, In-Ja L; DeLucia, Maria et al. (2014) Binding of HIV-1 Vpr protein to the human homolog of the yeast DNA repair protein RAD23 (hHR23A) requires its xeroderma pigmentosum complementation group C binding (XPCB) domain as well as the ubiquitin-associated 2 (UBA2) domain. J Biol Chem 289:2577-88
Henning, Matthew S; Dubose, Brittany N; Burse, Mallori J et al. (2014) In vivo functions of CPSF6 for HIV-1 as revealed by HIV-1 capsid evolution in HLA-B27-positive subjects. PLoS Pathog 10:e1003868
Vorontsov, Ivan I; Wu, Ying; DeLucia, Maria et al. (2014) Mechanisms of allosteric activation and inhibition of the deoxyribonucleoside triphosphate triphosphohydrolase from Enterococcus faecalis. J Biol Chem 289:2815-24
Sharaf, Naima G; Poliner, Eric; Slack, Ryan L et al. (2014) The p66 immature precursor of HIV-1 reverse transcriptase. Proteins 82:2343-52
Maertens, Goedele N; Cook, Nicola J; Wang, Weifeng et al. (2014) Structural basis for nuclear import of splicing factors by human Transportin 3. Proc Natl Acad Sci U S A 111:2728-33
Hou, Guangjin; Gupta, Rupal; Polenova, Tatyana et al. (2014) A Magic-Angle Spinning NMR Method for the Site-Specific Measurement of Proton Chemical-Shift Anisotropy in Biological and Organic Solids. Isr J Chem 54:171-183
Suiter, Christopher L; Paramasivam, Sivakumar; Hou, Guangjin et al. (2014) Sensitivity gains, linearity, and spectral reproducibility in nonuniformly sampled multidimensional MAS NMR spectra of high dynamic range. J Biomol NMR 59:57-73
Mitra, Mithun; Hercík, Kamil; Byeon, In-Ja L et al. (2014) Structural determinants of human APOBEC3A enzymatic and nucleic acid binding properties. Nucleic Acids Res 42:1095-110
Ishima, Rieko (2014) A probe to monitor performance of ¹?N longitudinal relaxation experiments for proteins in solution. J Biomol NMR 58:113-22

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