Imaging Core, directed by Zandrea Ambrose and Simon Watkins with collaborative effort from Peijun Zhang. i. Service mission: The Imaging Core will visualize the intracellular dynamics of HIV-i components during the processes of HIV-l CA core uncoating, RTxn, and PIC nuclear entry, by live-cell and high-resolution fixed cell microscopy, using existing and novel technologies. The Core will use labeled virions and host cell proteins of PCHPI-selected interest for conducting imaging studies and will develop novel techniques to track postentry events, visualizing viral protein and nucleic acids that interact with host proteins in vivo, using a complete array of super-resolution, optical sectioning, and wide field approaches in close collaboration with the technology development program.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Specialized Center (P50)
Project #
5P50GM082251-08
Application #
8727038
Study Section
Special Emphasis Panel (ZRG1-AARR-K)
Project Start
Project End
Budget Start
2014-08-01
Budget End
2015-07-31
Support Year
8
Fiscal Year
2014
Total Cost
$369,780
Indirect Cost
$125,701
Name
University of Pittsburgh
Department
Type
DUNS #
004514360
City
Pittsburgh
State
PA
Country
United States
Zip Code
15213
Zhao, Gongpu; Zhang, Peijun (2014) CryoEM analysis of capsid assembly and structural changes upon interactions with a host restriction factor, TRIM5?. Methods Mol Biol 1087:13-28
Jung, Jinwon; Byeon, In-Ja L; DeLucia, Maria et al. (2014) Binding of HIV-1 Vpr protein to the human homolog of the yeast DNA repair protein RAD23 (hHR23A) requires its xeroderma pigmentosum complementation group C binding (XPCB) domain as well as the ubiquitin-associated 2 (UBA2) domain. J Biol Chem 289:2577-88
Henning, Matthew S; Dubose, Brittany N; Burse, Mallori J et al. (2014) In vivo functions of CPSF6 for HIV-1 as revealed by HIV-1 capsid evolution in HLA-B27-positive subjects. PLoS Pathog 10:e1003868
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Sharaf, Naima G; Poliner, Eric; Slack, Ryan L et al. (2014) The p66 immature precursor of HIV-1 reverse transcriptase. Proteins 82:2343-52
Maertens, Goedele N; Cook, Nicola J; Wang, Weifeng et al. (2014) Structural basis for nuclear import of splicing factors by human Transportin 3. Proc Natl Acad Sci U S A 111:2728-33
Hou, Guangjin; Gupta, Rupal; Polenova, Tatyana et al. (2014) A Magic-Angle Spinning NMR Method for the Site-Specific Measurement of Proton Chemical-Shift Anisotropy in Biological and Organic Solids. Isr J Chem 54:171-183
Suiter, Christopher L; Paramasivam, Sivakumar; Hou, Guangjin et al. (2014) Sensitivity gains, linearity, and spectral reproducibility in nonuniformly sampled multidimensional MAS NMR spectra of high dynamic range. J Biomol NMR 59:57-73
Mitra, Mithun; Hercík, Kamil; Byeon, In-Ja L et al. (2014) Structural determinants of human APOBEC3A enzymatic and nucleic acid binding properties. Nucleic Acids Res 42:1095-110
Ishima, Rieko (2014) A probe to monitor performance of ¹?N longitudinal relaxation experiments for proteins in solution. J Biomol NMR 58:113-22

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